Temporal control of xyloglucan self-assembly into layered structures by radiation-induced degradation

Todaro, Simona; Sabatino, Maria Antonietta; Mangione, Maria Rosalia; Picone, Pasquale; Giacinto, Maria Laura Di; Bulone, Donatella; Dispenza, Clelia

doi:10.1016/j.carbpol.2016.07.005

Cited by 15 publications

(4 citation statements)

References 30 publications

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“…4e ) and CXG_PA-H3 5:1 ( Fig. 4f ), as observed also for non-carboxylated XGs [ 37 , 38 , 57 , 58 ], to isotropic and interconnected for CXG_PA-H3 1:1 ( Fig. 4c ) and CXG_PA-H3 2:1 ( Fig.…”

Section: Resultssupporting

confidence: 72%

Carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing

Ajovalasit

Redondo-Gómez

Sabatino

et al. 2021

Regenerative Biomaterials

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Hydrogel wound dressings can play critical roles in wound healing protecting the wound from trauma or contamination and providing an ideal environment to support the growth of endogenous cells and promote wound closure. This work presents a self-assembling hydrogel dressing that can assist the wound repair process mimicking the hierarchical structure of skin extracellular matrix. To this aim, the co-assembly behaviour of a carboxylated variant of xyloglucan (CXG) with a peptide amphiphile (PA-H3) has been investigated to generate hierarchical constructs with tuneable molecular composition, structure, and properties. Transmission electron microscopy and circular dichroism at a low concentration shows that CXG and PA-H3 co-assemble into nanofibres by hydrophobic and electrostatic interactions and further aggregate into nanofibre bundles and networks. At a higher concentration, CXG and PA-H3 yield hydrogels that have been characterized for their morphology by scanning electron microscopy and for the mechanical properties by small-amplitude oscillatory shear rheological measurements and compression tests at different CXG/PA-H3 ratios. A preliminary biological evaluation has been carried out both in vitro with HaCat cells and in vivo in a mouse model.

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“…4e ) and CXG_PA-H3 5:1 ( Fig. 4f ), as observed also for non-carboxylated XGs [ 37 , 38 , 57 , 58 ], to isotropic and interconnected for CXG_PA-H3 1:1 ( Fig. 4c ) and CXG_PA-H3 2:1 ( Fig.…”

Section: Resultssupporting

confidence: 72%

Carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing

Ajovalasit

Redondo-Gómez

Sabatino

et al. 2021

Regenerative Biomaterials

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“…Because of their relatively small space steric hindrance and higher spin degree of freedom, linear α- or β-(1-3)-glucans show the random thread chain conformation. Moreover, β-(1-3)-glucans tend to form helix conformations because of the numerous hydrogen bonds on their side chains; for example, for lentinan composed of β-(1-6)-branched β-(1-3)- d -glucans, the Atomic Force Microscope (AFM) image ( Figure 2 ) shows that lentinan exists as a helix in aqueous solution [ 2 ], curdlan is primarily composed of glucose units with linear -(1,3)-glycosidic linkages [ 3 ], and xyloglucan (XG) is a hemicellulose with a backbone formed by -(1,4)- d -glucan [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

β-Glucans: Relationships between Modification, Conformation and Functional Activities

et al. 2017

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β-glucan is a type of polysaccharide which widely exists in bacteria, fungi, algae, and plants, and has been well known for its biological activities such as enhancing immunity, antitumor, antibacterial, antiviral, and wound healing activities. The conformation of β-glucan plays a crucial role on its biological activities. Therefore, β-glucans obtained from different sources, while sharing the same basic structures, often show different bioactivities. The basic structure and inter-molecular forces of polysaccharides can be changed by modification, which leads to the conformational transformation in solution that can directly affect bioactivity. In this review, we will first determine different ways to modify β-glucan molecules including physical methods, chemical methods, and biological methods, and then reveal the relationship of the flexible helix form of the molecule chain and the helix conformation to their bioactivities. Last, we summarize the scientific challenges to modifying β-glucan’s conformation and functional activity, and discuss its potential future development.

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“…The present study analyzes the impact of absorbed dose and irradiation conditions (aqueous solution, humid and dry powders) on the molecular weight distribution of xyloglucan. The interest in xyloglucan stems from its use as soluble dietary bers, a food class with bene cial health effects such as blood cholesterol lowering and obesity prevention (Zhu et al, 2023) and its promising potential applications in the biomedical eld, as a hydrogel precursor for tissue engineering (Dispenza et al, 2017;Muscolino et al, 2021;Nisbet et al, 2006;Todaro et al, 2016), component of wound dressing formulations (Ajovalasit et al, 2021(Ajovalasit et al, , 2018Picone et al, 2019), and as a source of fragments with interesting antioxidant properties (Limsangouan et al, 2019). As reference compounds, carboxylated xyloglucan and hydroxypropyl cellulose were used.…”

Section: Introductionmentioning

confidence: 99%

The role of water in radiation-induced fragmentation of cellulosic backbone polysaccharides

Muscolino,

Sabatino,

Jonsson

et al. 2023

Cellulose

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Xyloglucan (XG) is a cellulosic backbone polysaccharide commercially used for food applications, but also widely investigated in biomedical applications, for its gelling properties and speci c biological activity. In this study, the possibility of using gamma radiation to cleave XG and generate lower molecular weight variants was explored. The impact of absorbed dose and irradiation conditions on the XG molecular weight distribution was investigated. Two other cellulosic polysaccharides, hydroxypropyl cellulose (HPC) and an oxidized variant of XG (CXG), were also studied for comparison. Before irradiation, the polymers were characterized with thermal gravimetric analysis (TGA) and, after irradiation, with gel ltration chromatography (GFC). The results showed that for XG irradiated in dilute aqueous solution, a dose of 10 Gy is su cient to signi cantly reduce the polymer molecular weight, while HPC is less affected by irradiation under identical conditions. When the polymers were irradiated in the solid form, either dry or humid, the reduction in average molecular weight is much less pronounced. Interestingly, for HPC the cleavage of the chains is more pronounced for the dry than for the humid powder. A similar behavior, but less pronounced, was observed for XG and CXG. Arguably, when water was present in the system as bound water it had a protective effect. This is probably due to energy transfer from the polymer to the bound water preventing chain scission. Indeed, humid HPC has more bound water than XG and CXG. Conversely, when water was present as solvent, water radiolysis products were able to e ciently induce depolymerization.

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Temporal control of xyloglucan self-assembly into layered structures by radiation-induced degradation

Cited by 15 publications

References 30 publications

Carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing

Carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing

β-Glucans: Relationships between Modification, Conformation and Functional Activities

The role of water in radiation-induced fragmentation of cellulosic backbone polysaccharides

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