Temporal Control of Gene Silencing by in ovo Electroporation

Baeriswyl, Thomas; Mauti, Olivier; Stoeckli, Esther T.

doi:10.1007/978-1-59745-191-8_16

Cited by 15 publications

(8 citation statements)

References 31 publications

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“…To start with, we tested three cationic lipids, DOTAP (Bollérot et al, 2006), DOTMA, and DODAC. (Inoue et al, 2001;Scaal et al, 2004;Baeriswyl et al, 2008;Sauka-Spengler and Barembaum, 2008). A variety of plasmids and nucleic acids can be delivered.…”

Section: Resultsmentioning

confidence: 99%

Development of high-concentration lipoplexes for in vivo gene function studies in vertebrate embryos

et al. 2011

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Here we report that highly concentrated cationic lipid/helper lipid‐nucleic acid complexes (lipoplexes) can facilitate reproducible delivery of a variety of oligonucleotides and plasmids to chicken embryos or to mouse embryonic mesenchyme. Specifically, liposomes composed of N,N‐dioleyl‐N,N‐dimethylammonium chloride (DODAC)/1,2 dioleoyl glycero‐3‐phosphorylethanolamine (DOPE) prepared at 18‐mM concentrations produced high levels of transfection of exogenous genes in vivo and in vitro. Furthermore, we report sufficient uptake of plasmids expressing interference RNA to decrease expression of both exogenous and endogenous genes. The simplicity of preparation, implementation, and relatively low toxicity of this transfection reagent make it an attractive alternative for developmental studies in post‐gastrulation vertebrate embryos. Developmental Dynamics 240:2108–2119, 2011. © 2011 Wiley‐Liss, Inc.

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Section: Resultsmentioning

confidence: 99%

Development of high-concentration lipoplexes for in vivo gene function studies in vertebrate embryos

et al. 2011

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“…There is no detailed analysis of avian Dicer specificity and activity, which have to be inferred indirectly from other results. Chicken Dicer can process both, long dsRNA and miRNA precursors, as evidenced by induction of RNAi with long dsRNA (Pekarik et al, 2003;Mauti et al, 2008) and hundreds of avian miRNAs in the miRBase.…”

Section: Dicermentioning

confidence: 99%

“…What can be inferred from those studies is that birds have the complete molecular mechanisms for canonical RNAi and can efficiently execute it. This is evidenced by efficient knock-downs with long dsRNA (Pekarik et al, 2003;Mauti et al, 2008).…”

Section: Rnaimentioning

confidence: 99%

“…Exogenous RNAi in vivo required non-physiological manipulations such as 1) plasmid or siRNA electroporation (Pekarik et al, 2003;Sato et al, 2004;Baeriswyl et al, 2008;Mauti et al, 2008;Stoeckli, 2011, 2012;Andermatt et al, 2014), 2) transfection (Dai et al, 2005;Lin et al, 2006a;Lin et al, 2013a;Wei et al, 2015), 3) recombinant virus (Lambeth et al, 2009b), or 4) recombinant lentivirus delivery (Haesler et al, 2007;Chen et al, 2009b). Altogether, these data suggest that exogenous RNAi would not be achieved by just exposing birds to small RNAs or their precursors in the environment or food.…”

Section: Rnaimentioning

confidence: 99%

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Literature review of baseline information to support the risk assessment of RNAi‐based GM plants

Pačes

Nič²,

Novotný³

et al. 2017

EFSA Supporting Publications

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This report is the outcome of an EFSA procurement aiming at investigating and summarising the state of knowledge on (I) the mode-of-action of dsRNA and miRNA pathways, (II) the potential for nontarget gene regulation by dsRNA-derived siRNAs or miRNAs, (III) the determination of siRNA pools in plant tissues and the importance of individual siRNAs for silencing. The report is based on a comprehensive and systematic literature search, starting with the identification and retrieval of~190,000 publications related to the research area and further filtered down with keywords to produce focused collections used for subsequent screening of titles and abstracts. The report is comprised of an (I) Introduction to the field of small RNAs, (II) a Data and Methodologies section containing strategies used for literature search and study selection, and (III) the Results of the literature review organized according to the three main procurement tasks. The outcome of the first task reviews dsRNA and miRNA pathways in mammals (including humans), birds, fish, arthropods, annelids, molluscs, nematodes, and plants. Eight taxon-dedicated chapters are based on~1,400 cumulative references chosen from~10,000 inspected titles and abstracts. We review conserved and divergent aspects of small RNA pathways and dsRNA responses in animals and plants including structure and function of key proteins as well as four basic mechanisms: genome-encoded posttranscriptional regulations (miRNA), degradation of RNAs by short interfering RNA pools generated from long dsRNA (RNAi), transcriptional silencing, and sequence-independent responses to dsRNA. The outcome of the second task focuses on base pairing between small RNAs and their target RNAs and predictability of biological effects of small RNAs in animals and plants. The outcome of the last task reviews methodology, siRNA pools, and movement of small RNAs in plants. Potential transfer of small RNAs between species and circulating miRNAs in mammals is described in the final chapter.

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“…Inject the dsRNA injection solution into the central canal of the neural tube using the glass needle (step 9). See Baeriswyl et al (2008), Wilson and Stoeckli (2012), and Andermatt and Stoeckli (2014) for a video (first reference) and a detailed description of the injection procedure.…”

Section: Injection Of Dsrna and Electroporation In Ovomentioning

confidence: 99%

Analysis of Cell‐Cell Contact Mediated by Ig Superfamily Cell Adhesion Molecules

et al. 2013

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Cell‐cell adhesion is a fundamental requirement for all multicellular organisms. The calcium‐independent cell adhesion molecules of the immunoglobulin superfamily (IgSF‐CAMs) represent a major subgroup. They consist of immunoglobulin folds alone or in combination with other protein modules, often fibronectin type‐III folds. More than 100 IgSF‐CAMs have been identified in vertebrates and invertebrates. Most of the IgSF‐CAMs are cell surface molecules that are membrane‐anchored either by a single transmembrane segment or by a glycosylphosphatidylinositol (GPI) anchor. Some of the IgSF‐CAMs also occur in soluble form, e.g., in the cerebrospinal fluid or in the vitreous fluid of the eye, due to naturally occurring cleavage of the GPI anchor or the membrane‐proximal peptide segment. Some IgSF‐CAMs, such as NCAM, occur in various forms that are generated by alternative splicing. This unit contains a series of protocols that have been used to study the function of IgSF‐CAMs in vitro and in vivo. Curr. Protoc. Cell Biol. 61:9.5.1‐9.5.85. © 2013 by John Wiley & Sons, Inc.

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Temporal Control of Gene Silencing by in ovo Electroporation

Cited by 15 publications

References 31 publications

Development of high-concentration lipoplexes for in vivo gene function studies in vertebrate embryos

Development of high-concentration lipoplexes for in vivo gene function studies in vertebrate embryos

Literature review of baseline information to support the risk assessment of RNAi‐based GM plants

Analysis of Cell‐Cell Contact Mediated by Ig Superfamily Cell Adhesion Molecules

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