Temporal, compositional, and functional differences in the microbiome of Bangkok subway air environment

Siriarchawatana, Paopit; Pumkaeo, Panyapon; Harnpicharnchai, Piyanun; Likhitrattanapisal, Somsak; Mayteeworakoon, Sermsiri; Boonsin, Worawongsin; Zhou, Xin; Liang, Junmin; Cai, Lei; Ingsriswang, Supawadee

doi:10.1016/j.envres.2022.115065

Cited by 9 publications

(2 citation statements)

References 71 publications

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“…After washing, the filter solution was concentrated onto 0.2‐μm pore size PES filter membrane (Pall Corporation, USA) using vacuum pump. DNA was then extracted following the protocol described by Crump et al (2003) and our pervious study (Siriarchawatana et al, 2023). Final DNA solution was measured the concentration by Qubit 2.0 (Invitrogen, USA) using the High Sensitivity double stranded DNA (HS dsDNA) kit.…”

Section: Methodsmentioning

confidence: 99%

“…There are currently 54 stations along the MRT route (Bangkok Expressway and Metro, 2018), and the MRT Sukhumvit (MRT-SKV) station was the busiest station in Bangkok with an annual ridership of around 35 million (Bangkok Metro Network, 2023) due to it being in the heart of Bangkok's business and shopping district and being an interconnecting point to the sky train system. Even though there is a recent interest in the bacteria communities and their functions in the Bangkok MRT environment (Siriarchawatana et al, 2023), the fungal communities in the Bangkok MRT environment have not been extensively examined and the information on airborne fungi was sorely lacking. Thus, the exploration of fungal compositions and their dynamics over time will immensely aid in monitoring and management of the airborne fungi in the subway station, which potentially impact the health of millions of passengers.…”

Section: Introductionmentioning

confidence: 99%

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Temporal dynamics in diversity and composition of the air mycobiome and dominant allergenic fungi in the subway environment

et al. 2023

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Analyzing and monitoring air microbiomes in the subway environment has a great impact on public health, especially in urban cities. In this study, temporal distribution profile of air mycobiome at Bangkok's Sukhumvit subway station (MRT‐SKV) was explored over 12 samplings during December 2021–November 2022 by the environmental DNA‐metabarcoding approach. The top 5 most abundant fungal genera identified were Yarrowia, Cladosporium, Hortaea, Cutaneotrichosporon, and Leptobacillum. Among the 496 genera found, 24 genera could be considered “core mycobiome” of the MRT‐SKV air, as they were consistently found in ≥85% of the samples. Many members of the core mycobiome constituted human commensal genera, but several of them can elicit allergic reactions in humans and thus pose a potential health risk. Cladosporium was the most abundant allergenic fungi found in the MRT‐SKV, which is in contrast to Malassezia, Ustilago, or Aspergillus being the most abundant allergenic fungi in subway environments in other countries. The fungal compositions in the subway environment differed temporally, and the abundances of allergenic fungi were the highest in samples obtained during the first and seventh samplings (done in December 2021–June 2022). These compositional variations were most likely influenced by air quality variables, including particulate matter (PM), temperature, and relative humidity, which showed seasonal variations. Finally, certain fungal genera were shown to co‐occur together in co‐occurrence network. Many of the allergenic fungal genera formed networks more prominently in rush hour than in other traffic hours. The co‐occurrence network suggested associations among certain fungal genera, such as the association between Malassezia–Nigrospora, Alternaria–Aspergillus, and Aspergillus–Cutaneotrichosporon in the MRT‐SKV bioaerosols. The implied associations between these fungi are worth further investigation since they may point toward their impact on human health (as they are opportunistic fungi found in human). Our results thus facilitate understanding the ecological and health impacts of fungal components of the subway air environment.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%