Temporal changes in the genotypes of methicillin-resistant Staphylococcus aureus strains isolated from a tertiary Malaysian hospital based on MLST, spa, and mec-associated dru typing

Lim, King Ting; Hanifah, Yasmin Abu; Yusof, Mohd Yasim Mohd; Goering, Richard V.; Thong, Kwai Lin

doi:10.1016/j.diagmicrobio.2012.05.033

Cited by 16 publications

(15 citation statements)

References 26 publications

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“…The remaining 7 dru types (dt13d, dt13g, dt14d, dt14i, dt10h, dt11a, and dt11c) were found in SCCmec type III strains. Among the 7 dru types, 3 (dt13g, dt13d, and dt14d) were also detected in MRSA strains isolated from 2 tertiary hospitals in Kuala Lumpur (18,38). Three dru types (dt14i, dt10h, and dt11c), including one novel dru type (dt11c), have never been reported in Malaysia.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains Isolated from a Tertiary Hospital in Terengganu, Malaysia

et al. 2012

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SUMMARY: Staphylococcus aureus is a persistent human pathogen responsible for a variety of infections ranging from soft-tissue infections to bacteremia. The objective of this study was to determine genetic relatedness between methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) strains. We isolated 35 MRSA and 21 MSSA strains from sporadic cases at the main tertiary hospital in Terengganu, Malaysia, screening them for the presence of virulence genes. Their genetic relatedness was determined by accessory gene regulator (agr) types, PCR-restriction fragment length polymorphism (RFLP) of the coa gene, pulsed-field gel electrophoresis (PFGE), S. aureus protein A (spa), and multilocus-sequence typing (MLST). We found that 57z of MRSA and 43z of MSSA strains harbored enterotoxin genes. The majority (87.5z) of the strains were agr type I. PCR-RFLP and PFGE genotyping of the coa gene revealed that MRSA strains were genetically related, whereas MSSA strains had higher heterogeneity. The combined genotype, MLST-spa type ST239-t037, was shared among MRSA and MSSA strains, indicating that MRSA strains could have evolved from MSSA strains. Two combined MLST-spa types were present in MRSA strains, whereas 7 different MLST-spa types were detected in MSSA strains, including 2 combined types (ST779-t878 and ST1179-t267) that have not been reported in Malaysia. In conclusion, enterotoxin genes were more prevalent in MRSA than in MSSA strains in the Terengganu hospital. The MSSA strains were genetically more diverse than the MRSA strains.

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Section: Discussionmentioning

confidence: 99%

“…We carried out purity and confirmation testing of the strains using a cefoxitin disk diffusion test and mannitol-salt agar as described by Lim et al (18). All strains were cultured in tryptone soy broth and stored in CryoVials containing 25z v/v glycerol (Invitrogen, Carlsbad, Calif., USA) at -859 C.…”

Section: Methodsmentioning

confidence: 99%

Comparison of Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains Isolated from a Tertiary Hospital in Terengganu, Malaysia

et al. 2012

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“…A well-characterized strain of S. aureus (MRSA0807-7) with genotype MLST type ST239, SCC mec type III, and spa type t421 or also known as ST239-III-t421 [16] was used for the optimization of LAMP assay. The sensitivity and specificity of the assay were evaluated with 124 clinical bacterial strains, which included 79 methicillin-resistant S. aureus (MRSA), 20 methicillin-sensitive S. aureus (MSSA), 5 Staphylococcus epidermidis , 5 Salmonella Typhimurium, 5 Shigella sonnei , 5 Listeria monocytogenes , and 5 Escherichia coli .…”

Section: Methodsmentioning

confidence: 99%

“…The MSSA strains were previously confirmed by BBL Staphyloslide-Latex Test (BD, USA) [17] while MRSA strains were confirmed by standard biochemical tests [16] with minor modifications. In brief, all MRSA strains were streaked on blood and mannitol salt agars and incubated overnight at 35°C.…”

Section: Methodsmentioning

confidence: 99%

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Loop-Mediated Isothermal Amplification Assay for the Rapid Detection ofStaphylococcus aureus

Lim

Teh

Thong

2013

BioMed Research International

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Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA), is an important human pathogen that produces a variety of toxins and causes a wide range of infections, including soft-tissue infections, bacteremia, and staphylococcal food poisoning. A loop-mediated isothermal amplification (LAMP) assay targeting the arcC gene of S. aureus was developed and evaluated with 119 S. aureus and 25 non-S. aureus strains. The usefulness of the assay was compared with the PCR method that targets spa and arcC genes. The optimal temperature for the LAMP assay was 58.5°C with a detection limit of 2.5 ng/μL and 102 CFU/mL when compared to 12.5 ng/μL and 103 CFU/mL for PCR (spa and arcC). Both LAMP and PCR assays were 100% specific, 100% sensitive, 100% positive predictive value (PPV), and 100% negative predictive value (NPV). When tested on 30 spiked blood specimens (21 MRSA, eight non-S. aureus and one negative control), the performance of LAMP and PCR was comparable: 100% specific, 100% sensitive, 100% PPV, and 100% NPV. In conclusion, the LAMP assay was equally specific with a shorter detection time when compared to PCR in the identification of S. aureus. The LAMP assay is a promising alternative method for the rapid identification of S. aureus and could be used in resource-limited laboratories and fields.

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Genomic analysis revealed a novel genotype of methicillin-susceptible Staphylococcus aureus isolated from a fatal sepsis case in dengue patient

Ngoi

Niek

Lee

et al. 2021

Sci Rep

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Staphylococcus aureus (S. aureus) is an opportunistic pathogen capable of causing serious health implications in susceptible individuals once it invades the host’s protective barriers. Methicillin-susceptible S. aureus (MSSA) often receives lesser attention although it has been frequently associated with serious infections in human. We aim to investigate the genomic features of a highly virulent yet pan susceptible MSSA strain (coded as HS-MSSA) which caused concurrent bacteraemia in a dengue patient, ultimately resulted in sepsis death of the patient. Whole genome sequence analysis was performed. The draft genome of HS-MSSA is approximately 2.78 Mb (GC content = 32.7%) comprising of 2637 predicted coding sequences. In silico genotyping of the HS-MSSA strain revealed a novel combined genotype (t091/ST2990). The HS-MSSA carries a SaPIn1-like pathogenicity island that harbours the staphylococcal enterotoxin and enterotoxin-like genes (sec3 and selL). The strain-specific β-lactamase (blaZ)-bearing plasmid region was identified in HS-MSSA. Core genome phylogeny showed that the HS-MSSA strain shared a common ancestry with the European MRSA clone. We report herein the genomic features of an MSSA lineage with novel genotype previously not reported elsewhere.

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Temporal changes in the genotypes of methicillin-resistant Staphylococcus aureus strains isolated from a tertiary Malaysian hospital based on MLST, spa, and mec-associated dru typing

Cited by 16 publications

References 26 publications

Comparison of Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains Isolated from a Tertiary Hospital in Terengganu, Malaysia

Comparison of Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains Isolated from a Tertiary Hospital in Terengganu, Malaysia

Loop-Mediated Isothermal Amplification Assay for the Rapid Detection ofStaphylococcus aureus

Genomic analysis revealed a novel genotype of methicillin-susceptible Staphylococcus aureus isolated from a fatal sepsis case in dengue patient

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