Temporal changes in gene expression in the liver of male plaice (Pleuronectes platessa) in response to exposure to ethynyl oestradiol analysed by macroarray and Real-Time PCR

Brown, Margaret; Robinson, Craig D.; Davies, Ian; Moffat, Colin F.; Redshaw, John; Craft, John A.

doi:10.1016/j.mrfmmm.2004.06.002

Cited by 36 publications

(12 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This concurs with the observations that E 2 and several other estrogenic compounds disrupt cytoskel-etal compounds in vitro [34][35][36]. In contrast, bactin expression has been shown to be increased in liver of plaice (Pleuronectes platessa) and zebrafish (Danio rerio) exposed to EE 2 [29,37], and in pituitary of Atlantic salmon (Salmo salar) exposed to E 2 and NP [38]. Expression of gapdh in livers of plaice and rainbow trout (Oncorhynchus mykiss) has also been shown to be repressed by various environmental estrogens, including EE 2 [28,29,39].…”

Section: Discussionsupporting

confidence: 89%

Untitled

2007

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 89%

Untitled

2007

View full text Add to dashboard Cite

show abstract

“…It is well known that increases in ROS production leads to apoptosis and activation of signaling pathways that elicit changes in gene expression, particularly transcription factors (Apel and Hirt 2004; Klaunig et al 2001). EE2-induced oxidative stress has been widely reported in several organisms, and can be associated with adverse effects in reproduction (Aris et al 2014; Brown et al 2004; Nadzialek and Kestemont 2010). …”

Section: Discussionmentioning

confidence: 99%

Dietary exposure of 17-alpha ethinylestradiol modulates physiological endpoints and gene signaling pathways in female largemouth bass (Micropterus salmoides)

et al. 2014

View full text Add to dashboard Cite

17alpha-ethinylestradiol (EE2), used for birth control in humans, is a potent estrogen that is found in wastewater at low concentrations (ng/L). EE2 has the ability to interfere with the endocrine system of fish, affecting reproduction which can result in population level effects. The objective of this study was to determine if dietary exposure to EE2 would alter gene expression patterns and key pathways in the liver and ovary and whether these could be associated with reproductive endpoints in female largemouth bass during egg development. Female LMB received 70 ng EE2/g feed (feed administered at 1% of body weight) for 60 days. EE2 dietary exposure significantly reduced plasma vitellogenin concentrations by 70%. Hepatosomatic and gonadosomatic indices were also decreased with EE2 feeding by 38.5% and 40%, respectively. Transcriptomic profiling revealed that there were more changes in steady state mRNA levels in the liver compared to the ovary. Genes associated with reproduction were differentially expressed such as vitellogenin in the liver and aromatase in the gonad. In addition, a set of genes related with oxidative stress (e.g. glutathione reductase and glutathione peroxidase) were identified as altered in the liver and genes associated with the immune system (e.g. complement component 1, and macrophage-inducible C-type lectin) were altered in the gonad. In a follow-up study with 0.2 ng EE2/g feed for 60 days, similar phenotypic and gene expression changes were observed that support these findings with the higher concentrations. This study provides new insights into how dietary exposure to EE2 interferes with endocrine signaling pathways in female LMB during a critical period of reproductive oogenesis.

show abstract

“…For the present gene profile study, rather than use a DNA chip (DNA microarray) (4-14), we chose a DNA macroarray for its superior ease of use and ability to make quantitative measurements (15)(16)(17)(18)(19). The former holds from several hundred to several tens of thousand dot-shaped mRNA measuring probes arranged on a 1 to 2-cm square plate, whereas the latter has a similar number of dot-shaped mRNA measuring probes, which, as the name suggests, are visible to the naked eye, arranged on a filter ~10x15 cm in size.…”

Section: -------------------------------------------------Gene Class mentioning

confidence: 99%

Gene profile analysis of colorectal cancer cell lines by cDNA macroarray

Sakai

Kameoka²,

Furukawa³

2007

Oncol Rep

View full text Add to dashboard Cite

Abstract. In order to identify high-risk groups of patients with cancer, understanding the mechanisms of invasion and metastasis from the viewpoint of gene expression is required; in particular, the changes in gene expression as tumours gain the ability to metastasise. Using a rat model of metastatic colorectal cancer (CRC), we determined the expression profiles of CRC cells that metastasised to the liver and the lung. In the hepatopulmonary metastasis model, metastasising ability increased with successive transplanted cell line generations. No significant differences in cellular proliferation ability were seen between the original cell line and succeeding metastatic cell lines. Analysis using cDNA macroarray showed that metastasising ability was associated with increased expression of integrin ß4, γ-catenin, Smad 7, Bax, Bcl-2, c-fos and TGF-·, and a particularly marked increased expression of TGF-ß, PDGFb, Cdk4 and Rho B. Expression of both Rho GDIß and Gelsolin was reduced. These results suggest that high metastasising ability does not derive from a single gene, but rather through accumulated changes in the expression of several different genes.

show abstract

Temporal changes in gene expression in the liver of male plaice (Pleuronectes platessa) in response to exposure to ethynyl oestradiol analysed by macroarray and Real-Time PCR

Cited by 36 publications

References 51 publications

Untitled

Untitled

Dietary exposure of 17-alpha ethinylestradiol modulates physiological endpoints and gene signaling pathways in female largemouth bass (Micropterus salmoides)

Gene profile analysis of colorectal cancer cell lines by cDNA macroarray

Contact Info

Product

Resources

About