Temporal Changes in Gene Expression after Injury in the Rat Retina

Vázquez-Chona, Félix; Song, Bong Keun; Geisert, Eldon E.

doi:10.1167/iovs.03-1047

Cited by 101 publications

(117 citation statements)

References 38 publications

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“…Recent studies have shown that levels of alpha-crystallins are considerably elevated in models of light toxicity and retinal tearing and suggested they play a crucial role in the protection of retinal tissue [27,28]. Alpha-crystallin may also play a significant role in the pathophysiology of neurodegenerative disorders.…”

Section: Expression Of Heat Shock Proteins In the Eyementioning

confidence: 99%

Impact of diabetes on alpha-crystallins and other heat shock proteins in the eye

Heise

Fort

2011

j ocul biol dis inform

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Diabetes and its related complications represent a major growing health concern and economic burden worldwide. Ocular manifestations of diabetes include cataractogenesis and retinopathy, the latter being the leading cause of blindness in the working-age population. Despite numerous studies and recent progress, the exact pathophysiology of the disease remains to be fully elucidated and development of new and improved therapeutic strategies for this chronic condition are greatly needed. Heat shock proteins (Hsps) are highly conserved families of proteins, which are generally regarded as protective molecules that play a wide variety of roles and can be expressed in response to different types of cellular stresses. In recent years, numerous studies have reported their implication in various ocular diseases including diabetic retinopathy. The present review focuses on the potential implication of Hsps in ocular diabetic complications and discusses their specific mechanisms of regulation with respect to their expression, functions and alteration during diabetes. The review will conclude by examining the potential of Hsps as therapeutic agents or targets for the treatment of diabetic retinopathy.

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Section: Expression Of Heat Shock Proteins In the Eyementioning

confidence: 99%

Impact of diabetes on alpha-crystallins and other heat shock proteins in the eye

Heise

Fort

2011

j ocul biol dis inform

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show abstract

“…Similarly, of the 27 reported genes between E18 and P14 in corticospinal motor neurons that were found in the RGC GeneChip dataset, only two were developmentally regulated in RGCs (Purkinje cell protein 4 and neurofilament-H). We also compared P21 RGC gene expression with adult retinal gene expression published previously (Vazquez-Chona et al, 2004) using triplicate biological samples and data from the Affymetrix U34A GeneChips (ϳ8800 probes). Of the 3566 genes present in at least two of three P21 RGC samples, most were also observed in retina (Fig.…”

Section: Retinal Ganglion Cell Gene Expressionmentioning

confidence: 99%

Disease Gene Candidates Revealed by Expression Profiling of Retinal Ganglion Cell Development

Wang

Kunzevitzky²,

Dugas³

et al. 2007

J. Neurosci.

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To what extent do postmitotic neurons regulate gene expression during development or after injury? We took advantage of our ability to highly purify retinal ganglion cells (RGCs) to profile their pattern of gene expression at 13 ages from embryonic day 17 through postnatal day 21. We found that a large proportion of RGC genes are regulated dramatically throughout their postmitotic development, although the genes regulated through development in vivo generally are not regulated similarly by RGCs allowed to age in vitro. Interestingly, we found that genes regulated by developing RGCs are not generally correlated with genes regulated in RGCs stimulated to regenerate their axons. We unexpectedly found three genes associated with glaucoma, optineurin, cochlin, and CYP1B1 (cytochrome P450, family 1, subfamily B, polypeptide 1), previously thought to be primarily expressed in the trabecular meshwork, which are highly expressed by RGCs and regulated through their development. We also identified several other RGC genes that are encoded by loci linked to glaucoma. The expression of glaucoma-linked genes by RGCs suggests that, at least in some cases, RGCs may be directly involved in glaucoma pathogenesis rather than indirectly involved in response to increased intraocular pressure. Consistent with this hypothesis, we found that CYP1B1 overexpression potentiates RGC survival.

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“…They are evolutionarily related to certain microorganism stress proteins and to vertebrate proteins associated with processes of cellular differentiation and morphological change (Wistow, 1995). Various β,γ-crystallins are present in low abundance in tissues other than the lens, most prominently in the retina (Andley, 2007) where their expression is reported to be upregulated following various injuries or stresses (Sakaguchi et al, 2003;Vazquez-Chona et al, 2004;Crabb et al, 2002). βB2-crystallin has recently been reported to be secreted by the retina in culture where it promotes the regrowth of retinal ganglion cell axons (Liedtke et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

βA3/A1-crystallin in astroglial cells regulates retinal vascular remodeling during development

Sinha

Klise

Sergeev

et al. 2008

Molecular and Cellular Neuroscience

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Vascular remodeling is a complex process critical to development of the mature vascular system. Astrocytes are known to be indispensable for initial formation of the retinal vasculature; our studies with the Nuc1 rat provide novel evidence that these cells are also essential in the retinal vascular remodeling process. Nuc1 is a spontaneous mutation in the Sprague-Dawley rat originally characterized by nuclear cataracts in the heterozygote and microphthalmia in the homozygote. We report here that the Nuc1 allele results from mutation of the βA3/A1-crystallin gene, which in the neural retina is expressed only in astrocytes. We demonstrate striking structural abnormalities in Nuc1 astrocytes with profound effects on the organization of intermediate filaments. While vessels form in the Nuc1 retina, the subsequent remodeling process required to provide a mature vascular network is deficient. Our data implicate βA3/A1-crystallin as an important regulatory factor mediating vascular patterning and remodeling in the retina.

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Temporal Changes in Gene Expression after Injury in the Rat Retina

Cited by 101 publications

References 38 publications

Impact of diabetes on alpha-crystallins and other heat shock proteins in the eye

Impact of diabetes on alpha-crystallins and other heat shock proteins in the eye

Disease Gene Candidates Revealed by Expression Profiling of Retinal Ganglion Cell Development

βA3/A1-crystallin in astroglial cells regulates retinal vascular remodeling during development

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