Temporal changes in circulating levels of plasma interleukin-8 during peripartum period in Murrah buffaloes (Bubalus bubalis) under subtropical climate

Abstract

The objective of this study was to elucidate the changes in circulating levels of plasma interleukin-8 (IL-8) during peripartum period in Murrah buffaloes (Bubalus bubalis). IL-8 was estimated in blood plasma of healthy peripartum Murrah buffaloes (n=6) on days ± 30, ± 15, ± 5, ± 3, ± 1 and 0 pre- and postpartum with respect to the day of parturition (day 0) in each of the two different seasons (hot-humid and spring). The mean microclimate Temperature-Humidity Index (THI) during spring season was significantly lower (p<0.01) than the corresponding THI in hot-humid season. In both the seasons, plasma IL-8 remained lower in prepartum period (≤ 46.56 ± 14.08 pg/ml during spring and ≤ 73.18 ± 18.56 pg/ml during hot-humid season) than in the postpartum period (≥ 51.41 ± 13.82 pg/ml during spring and ≥ 84.13 ± 16.97 pg/ml in hot humid season). During spring, the IL-8 levels were significantly higher (P<0.05) on days+5 and +15 postpartum in comparison to the IL-8 levels on days -30, -5, and -3 prepartum. During hot-humid season, IL-8 level was significantly higher (P<0.05) on day +30 as compared to the IL-8 levels on days -30 and -5 prepartum. The correlation between IL-8 and mean microclimate THI was significant (r=0.25, P<0.01). From the results, it is concluded that peripartum period in buffaloes is associated with an inflammatory response leading to significantly higher plasma IL-8 during parturition and postpartum period than in the pre-partum period.

Concentrations of serum amyloid A, haptoglobin, tumour necrosis factor and interleukin-1 and -6 in Anatolian buffaloes naturally infected with dermatophytosis-

“…The author concluded that the increased serum IL-6 and IL-8 levels in infected buffaloes suggest that these cytokines may play a role in the immune reaction during liver fluke infection in some species. Kumar et al (2011) examined IL-8 levels in Murrah buffaloes during the peripartum period and reported that IL-8 was high in the postpartum period. They explained that the rise in IL-8 levels in buffaloes is a cumulative immune response not only to the physiological stress of parturition and uterine involution during the postpartum period, but also to the high thermal stress from the environment.…”