Temporal, cell-specific, and tissue-preferential expression of myrosinase genes during embryo and seedling development in Sinapis alba

Xue, Jiaping; Pihlgren, Ulla; Rask, Lars

doi:10.1007/bf00240900

Cited by 23 publications

(36 citation statements)

References 16 publications

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“…Double-staining with histochemical techniques employing reagents such as 'Millon's reagent' and antibodies against myrosinase showed that myrosin cells contain myrosinase [55,127]. It was later shown by in situ hybridization experiments that myrosinase transcripts are present in these cells and that they presumably synthesize the enzyme [35,68,144]. In mesophyll tissue from cotyledons, the shape of the myrosin cells may vary from isodiametric cells of normal size to elongated cells with an area 7-8 times larger than the surrounding cells.…”

Section: The Myrosin Cellmentioning

confidence: 98%

“…The expression of the three myrosinase gene subfamilies has been studied in B. napus by northern blot and in situ hybridization analyses using subfamilyspecific probes [34,35,68]. In parallel, the localization of MA and MB transcripts to different organs of S. alba was documented [144]. The MA myrosinase genes in both species were exclusively expressed in the myrosin cells of the seed embryo, preferentially in the axis.…”

Section: Distribution Of Myrosinase Isoenzymesmentioning

confidence: 98%

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Myrosinase: gene family evolution and herbivore defense in Brassicaceae

Rask

Andréasson

Ekbom

et al. 2000

Plant Molecular Evolution

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319

358

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Glucosinolates are a category of secondary products present primarily in species of the order Capparales. When tissue is damaged, for example by herbivory, glucosinolates are degraded in a reaction catalyzed by thioglucosidases, denoted myrosinases, also present in these species. Thereby, toxic compounds such as nitriles, isothiocyanates, epithionitriles and thiocyanates are released. The glucosinolate-myrosinase system is generally believed to be part of the plant's defense against insects, and possibly also against pathogens. In this review, the evolution of the system and its impact on the interaction between plants and insects are discussed. Further, data suggesting additional functions in the defense against pathogens and in sulfur metabolism are reviewed.

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Section: The Myrosin Cellmentioning

confidence: 98%

Section: Distribution Of Myrosinase Isoenzymesmentioning

confidence: 98%

Myrosinase: gene family evolution and herbivore defense in Brassicaceae

Rask

Andréasson

Ekbom

et al. 2000

Plant Molecular Evolution

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319

358

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“…Studies on the expression of MA and MB myrosinases in S. alba during embryo and seedling development by in situ and northern analyses clearly showed that MA and MB genes followed similar temporal profiles during embryogenesis, but MB mRNA was present in considerably higher amounts than MA mRNA [37]. In the embryo, both MA and MB genes are transcribed in cotyledons and axis.…”

Section: Introductionmentioning

confidence: 99%

The myrosinase gene family in Arabidopsis thaliana: gene organization, expression and evolution

et al. 1995

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Myrosinase (thioglucoside glucohydrolase, EC 3.2.3.1.) is in Brassicaceae species such as Brassica napus and Sinapis alba encoded by two differentially expressed gene families, MA and MB, consisting of about 4 and 10 genes, respectively. Southern blot analysis showed that Arabidopsis thaliana contains three myrosinase genes. These genes were isolated from a genomic library and two of them, TGG1 and TGG2, were sequenced. They were found to be located in an inverted mode with their 3' ends 4.4 kb apart. Their organization was highly conserved with 12 exons and 11 short introns. Comparison of nucleotide sequences of TGG1 and TGG2 exons revealed an overall 75% similarity. In contrast, the overall nucleotide sequence similarity in introns was only 42%. In intron 1 the unusual 5' splice border GC was used. Phylogenetic analyses using both distance matrix and parsimony programs suggested that the Arabidopsis genes could not be grouped with either MA or MB genes. Consequently, these two gene families arose only after Arabidopsis had diverged from the other Brassicaceae species. In situ hybridization experiments showed that TGG1 and TGG2 expressing cells are present in leaf, sepal, petal, and gynoecium. In developing seeds, a few cells reacting with the TGG1 probe, but not with the TGG2 probe, were found indicating a partly different expression of these genes.

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“…This analysis can at present only be carried out with the genes from Arabidopsis, since this is the only species in which all myrosinase genes have been isolated and at least partly characterized [20]. The analysis was carried out using RNA [22] isolated from both vegetative tissue, seeds and siliques of different developmental stages harvested from A. thaliana ecotype Columbia. The following primers were used: Forward primer 1: 5'-CCA TGA AGC TTC TTA TGC-3', TGG1 gene-specific primer (1783-1800) [20]; forward primer 2: 5'-CAT GAA GCT TCT T G G GT-3', TGG2 gene-specific primer corresponding to 1467-1483; reversed primer 3: 5'-GAG TCA CAA GTA G T G TCT-3', consensus primer derived from exon 3 (2265-2282 in the TGG1 sequence; 1994-2011 in the TGG2 sequence); forward primer 4: 5'-CTT CTG CTT ACC A G G CAT-3', consensus primer derived from exon 1 (1942)(1943)(1944)(1945)(1946)(1947)(1948)(1949)(1950)(1951)(1952)(1953)(1954) in the TGG1 sequence; 1628-1645 in the TGG2 sequence); reversed primer 5: 5'CTG G G T ATC G G T GAG TG-3', consensus primer derived from exon 2 of T G G 1 (2104-2120) and TGG2 (1783-1799) to amplify TGG3; forward primer 6: 5'-CTT CAT CTT CGG TGT TGC-3', consensus primer derived from exon 1 of TGG1 (1922)(1923)(1924)(1925)(1926)(1927)(1928)(1929)(1930)(1931)(1932)(1933)(1934)(1935)(1936)(1937)(1938)(1939) and TGG2 (1608-1625) to amplify TGG3 [20].…”

mentioning

confidence: 99%

The unusual 5? splicing border GC is used in myrosinase genes of the Brassicaceae

Xue

Rask

1995

Plant Mol Biol

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Myrosinase (thioglucosidase glucohydrolase; EC 3.2.3.1) is a group of isoenzymes in the Brassicaceae, which hydrolyze glucosinolates. Genes encoding myrosinase contain 12 exons and 11 introns. Sequence comparison of two myrosinase genes from Arabidopsis thaliana, TGG1 and TGG2, with the corresponding cDNA from leaves, showed preferential use of a GC dinucleotide as the 5' splicing border in intron 1 instead of an adjacent GT dinucleotide four bp further 3'. This 5' GC splice site is conserved in all seven myrosinase genes characterized from three different species. Likewise, in the 3' region of intron 1 two AG dinucleotides are located seven bp apart. Only the most 5' of these dinucleotides was found to be used in splicing. Sequence analyses of TGG1 cDNA isolated from seeds, siliques and vegetative tissue using reverse transcription PCR showed that the splicing pattern of this intron is identical in these tissues for TGG1. The GT and the most 3' AG dinucleotides mentioned above have been assumed to be the intron borders of intron 1 in several myrosinase genes. The present investigation shows that this assumption is not correct.

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Temporal, cell-specific, and tissue-preferential expression of myrosinase genes during embryo and seedling development in Sinapis alba

Cited by 23 publications

References 16 publications

Myrosinase: gene family evolution and herbivore defense in Brassicaceae

Myrosinase: gene family evolution and herbivore defense in Brassicaceae

The myrosinase gene family in Arabidopsis thaliana: gene organization, expression and evolution

The unusual 5? splicing border GC is used in myrosinase genes of the Brassicaceae

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