Temporal and tissue specific gene expression patterns of the zebrafish kinesin-1 heavy chain family, kif5s, during development

Campbell, Philip D.; Marlow, Florence

doi:10.1016/j.gep.2013.05.002

Cited by 26 publications

(28 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1D-F). Together with our previous studies showing that kif5Ba is the main maternally expressed kif5 (Campbell and Marlow, 2013), these results indicate that Kif5Ba binds Buc and might mediate Buc localization in embryos.…”

Section: Resultssupporting

confidence: 87%

Kinesin-1 interacts with Bucky ball to form germ cells and is required to pattern the zebrafish body axis

et al. 2015

Self Cite

View full text Add to dashboard Cite

In animals, specification of the primordial germ cells (PGCs), the stem cells of the germline, is required to transmit genetic information from one generation to the next. Bucky ball (Buc) is essential for germ plasm (GP) assembly in oocytes and its overexpression results in excess PGCs in zebrafish embryos. However, the mechanistic basis for the excess PGCs in response to Buc overexpression, and whether endogenous Buc functions during embryogenesis are unknown. Here we show that endogenous Buc, like GP and overexpressed Buc-GFP, accumulates at embryonic cleavage furrows. Furthermore, we show that the maternally expressed zebrafish Kinesin-1 Kif5Ba is a binding partner of Buc and that maternal kif5Ba (Mkif5Ba) plays an essential role in germline specification in vivo. Specifically, Mkif5Ba is required to recruit GP to cleavage furrows and thereby specifies PGCs. Moreover, Mkif5Ba is required to enrich Buc at cleavage furrows and for Buc’s ability to promote excess PGCs, providing mechanistic insight into how Buc functions to assemble embryonic GP. In addition, we show that Mkif5Ba is also essential for dorsoventral (DV) patterning. Specifically, Mkif5Ba promotes formation of the parallel vegetal microtubule array required to asymmetrically position dorsal determinants (DDs) towards the prospective dorsal side. Interestingly, while Syntabulin and wnt8a translocation depend on kif5Ba, grip2a translocation does not, providing evidence for two distinct mechanisms by which DDs may be asymmetrically distributed. These studies identify essential roles for maternal Kif5Ba in PGC specification and DV patterning and provide mechanistic insight into Buc functions during early embryogenesis.

show abstract

Section: Resultssupporting

confidence: 87%

Kinesin-1 interacts with Bucky ball to form germ cells and is required to pattern the zebrafish body axis

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…Using in situ hybridization, we detected mRNA expression of this gene from 24 h postfertilization onward in the spinal cord (Fig. 3A), consistent with a recently published expression pattern (Campbell and Marlow 2013). At 3 dpf, kif5aa is broadly expressed in the brain, while BAC transgenesis using the medaka (Oryzias latipes) ortholog showed additional kif5aa transcription in the spinal cord and motoneurons at later stages of development (Kawasaki et al 2012).…”

Section: Targeted Knock-in At the Zebrafish Kif5aa Locussupporting

confidence: 87%

Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair

et al. 2013

View full text Add to dashboard Cite

Sequence-specific nucleases like TALENs and the CRISPR/Cas9 system have greatly expanded the genome editing possibilities in model organisms such as zebrafish. Both systems have recently been used to create knock-out alleles with great efficiency, and TALENs have also been successfully employed in knock-in of DNA cassettes at defined loci via homologous recombination (HR). Here we report CRISPR/Cas9-mediated knock-in of DNA cassettes into the zebrafish genome at a very high rate by homology-independent double-strand break (DSB) repair pathways. After co-injection of a donor plasmid with a short guide RNA (sgRNA) and Cas9 nuclease mRNA, concurrent cleavage of donor plasmid DNA and the selected chromosomal integration site resulted in efficient targeted integration of donor DNA. We successfully employed this approach to convert eGFP into Gal4 transgenic lines, and the same plasmids and sgRNAs can be applied in any species where eGFP lines were generated as part of enhancer and gene trap screens. In addition, we show the possibility of easily targeting DNA integration at endogenous loci, thus greatly facilitating the creation of reporter and loss-of-function alleles. Due to its simplicity, flexibility, and very high efficiency, our method greatly expands the repertoire for genome editing in zebrafish and can be readily adapted to many other organisms.

show abstract

“…This multicolor labeling strategy can be easily applied to neurobiology studies to induce targeted mutations in single neurons and directly compare loss-of-function and wild-type phenotypes in the same animal. To test this potential application, we targeted the genomic locus coding for the motor protein Kinesin family member 5A, a (kif5aa) (Campbell and Marlow 2013;Auer et al 2015), whose inactivation triggers the reduction of RGC axon arbor complexity via a cell-autonomous mechanism (Auer et al 2015). To target the kif5aa gene with the 2C-Cas9 system in single RGC, we used the Tg(isl2b:gal4) line.…”

Section: Crispr/cas9 and Gal4/uas Combination For Cell-specific Gene mentioning

confidence: 99%

CRISPR/Cas9-Mediated Knockin and Knockout in Zebrafish

Albadri

Santis

Donato

et al. 2017

Research and Perspectives in Neurosciences

View full text Add to dashboard Cite

The zebrafish (Danio rerio) has emerged in recent years as a powerful vertebrate model to study neuronal circuit development and function, thanks to its relatively small size, rapid external development and translucency. These features allow the easy application of in vivo microscopy analysis and optical perturbation of neuronal function. So far, genetic manipulation in zebrafish has been limited to the generation of constitutive loss-of-function alleles and transgenic models.

show abstract

Temporal and tissue specific gene expression patterns of the zebrafish kinesin-1 heavy chain family, kif5s, during development

Cited by 26 publications

References 37 publications

Kinesin-1 interacts with Bucky ball to form germ cells and is required to pattern the zebrafish body axis

Kinesin-1 interacts with Bucky ball to form germ cells and is required to pattern the zebrafish body axis

Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair

CRISPR/Cas9-Mediated Knockin and Knockout in Zebrafish

Contact Info

Product

Resources

About