Temporal and spatial variation in MSP1 clonal composition of Plasmodium falciparum in districts of Assam, Northeast India

Baruah, Satyabrat Malla Bujar; Lourembam, Sonia D.; Sawian, Clara E.; Baruah, Indra; Goswami, Dibakar

doi:10.1016/j.meegid.2009.05.006

Cited by 16 publications

(14 citation statements)

References 33 publications

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“…25 A positive association between MOI and endemicity of P. falciparum has been reported in several studies, 8,9,11,12,17,23 although some exceptions where high MOI was observed in hypoendemic areas are known. 14,19 In the present study, MOI was 1.51, 1.25, and 1.08 for msp1, msp2, and glurp, respectively. In southeast Asia, MOI of P. falciparum for hypoendemic regions ranges from 1.00 to 2.70.…”

Section: Discussionsupporting

confidence: 47%

“…[7][8][9][10][11][12][13][14][15][16][17] Since treatment with drugs of malaria patients is an integral part of malaria control and prevention strategy and the selected markers can be used as drug efficacy markers, knowing the baseline population structure of circulating parasites in an endemic country will aid in monitoring change in transmission pattern as well as efficacy of drugs that are in use. In this study, pretreatment patients were enrolled and genotyped to know parasite population structure and provide a baseline data for future reference.…”

Section: Discussionmentioning

confidence: 99%

“…High polymorphism in merozoite surface protein 1 (msp1), merozoite surface protein 2 (msp2), and glutamaterich protein (glurp) genes have been shown in genetic diversity studies in different malaria-endemic countries from Asia to Africa. [7][8][9][10][11][12][13][14][15][16][17] Large allelic polymorphism has been reported in the block 2 of the msp1, the central repetitive domain of the msp2, and region II of the glurp. In msp1, three distinct allelic families have been described: K1, MAD20, and RO33 while in msp2, two allelic families, FC27 and 3D7/IC, have been described.…”

Section: Introductionmentioning

confidence: 99%

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Plasmodium falciparum Genetic Diversity in Bangladesh Does Not Suggest a Hypoendemic Population Structure

Alam¹,

Elahi²,

Mohon³

et al. 2016

The American Society of Tropical Medicine and Hygiene

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Abstract. Despite the recommendation for the use of merozoite surface protein 1 (msp1), merozoite surface protein 2 (msp2), and glutamate-rich protein (glurp) genes as markers in drug efficacy studies by World Health Organization and their limited use in Bangladesh, the circulating Plasmodium falciparum population genetic structure has not yet been assessed in Bangladesh. This study presents a comprehensive report on the circulating P. falciparum population structure based on msp1, msp2, and glurp polymorphic gene markers in Bangladesh. Among the 130 pretreatment (day 0) P. falciparum samples from seven malaria-endemic districts, 14 distinct genotypes were observed for msp1, 20 for msp2, and 13 for glurp. Polyclonal infection was reported in 94.6% (N = 123) of the samples. Multiplicity of infection (MOI) for msp1 was the highest (1.5) among the MOIs of the markers. The heterozygosity for msp1, msp2, and glurp was 0.89, 0.93, and 0.83, respectively. Data according to different malaria-endemic areas are also presented and discussed. Bangladesh is considered as a malaria-hypoendemic country. However, the prevalence of polyclonal infection and the genetic diversity of P. falciparum do not represent hypoendemicity.

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Section: Discussionsupporting

confidence: 47%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Plasmodium falciparum Genetic Diversity in Bangladesh Does Not Suggest a Hypoendemic Population Structure

Alam¹,

Elahi²,

Mohon³

et al. 2016

The American Society of Tropical Medicine and Hygiene

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“…Moreover, the fact that msp2 is under immune selection might partially mask the true genetic diversity of the populations investigated here. It would also be important to evaluate the temporal evolution of the genetic structures, as time has been identified as a diversity factor almost as important than geographical location in various transmission settings [15,48]. …”

Section: Discussionmentioning

confidence: 99%

“…intercontinental or spread within one or several countries, see [7-11]), or a small number of epidemiologically very distinct locations [12-15] and reported observations on the complex interplay between transmission intensity, the multiplicity of infection (MOI) with Plasmodium and the genetic structure of local parasite populations. High transmission settings have been frequently, but not always, associated with greater genetic diversity and MOI [16,17].…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Plasmodium falciparum among school-aged children from the Man region, western Côte d’Ivoire

Mara

Silué

Raso

et al. 2013

Malar J

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BackgroundThe genetic diversity of Plasmodium falciparum allows the molecular discrimination of otherwise microscopically identical parasites and the identification of individual clones in multiple infections. The study reported here investigated the P. falciparum multiplicity of infection (MOI) and genetic diversity among school-aged children in the Man region, western Côte d’Ivoire.MethodsBlood samples from 292 children aged seven to 15 years were collected in four nearby villages located at altitudes ranging from 340 to 883 m above sea level. Giemsa-stained thick and thin blood films were prepared and examined under a microscope for P. falciparum prevalence and parasitaemia. MOI and genetic diversity of the parasite populations were investigated using msp2 typing by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).ResultsPlasmodium falciparum prevalence and parasitaemia were both found to be significantly lower in the highest altitude village. Genotyping of the isolates revealed 25 potentially new msp2 alleles. MOI varied significantly across villages but did not correlate with altitude nor children’s age, and only to a limited extent with parasitaemia. An analysis of molecular variance (AMOVA) indicated that a small, but close to statistical significance (p = 0.07), fraction of variance occurs specifically between villages of low and high altitudes.ConclusionsHigher altitude was associated with lower prevalence of P. falciparum but not with reduced MOI, suggesting that, in this setting, MOI is not a good proxy for transmission. The evidence for partially parted parasite populations suggests the existence of local geographical barriers that should be taken into account when deploying anti-malarial interventions.

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Differential association of KIR gene loci to risk of malaria in ethnic groups of Assam, Northeast India

Lourembam¹,

Sawian²,

Baruah³

2011

Infection, Genetics and Evolution

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Temporal and spatial variation in MSP1 clonal composition of Plasmodium falciparum in districts of Assam, Northeast India

Cited by 16 publications

References 33 publications

Plasmodium falciparum Genetic Diversity in Bangladesh Does Not Suggest a Hypoendemic Population Structure

Plasmodium falciparum Genetic Diversity in Bangladesh Does Not Suggest a Hypoendemic Population Structure

Genetic diversity of Plasmodium falciparum among school-aged children from the Man region, western Côte d’Ivoire

Differential association of KIR gene loci to risk of malaria in ethnic groups of Assam, Northeast India

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