Temporal and Spatial Spread of Soybean mosaic virus (SMV) in Soybeans Transformed with the Coat Protein Gene of SMV

Abstract

Soybean lines transformed with the coat protein (CP) gene of Soybean mosaic virus (SMV) were evaluated for SMV resistance by quantifying the temporal and spatial spread of SMV strain AL-5 released from a point source in the field. The temporal spread of SMV within field plots during 1999 and 2000 was quantified by enzyme-linked immunosorbent assay. The Gompertz model most appropriately described temporal spread. Two SMV CP transformed lines (genotypes) had significantly lower infection rates and significantly lower final SMV incidence values (P </= 0.05) compared with controls that did not contain the CP gene. Ordinary runs analysis revealed that the spatial pattern of SMV-infected quadrats was more clustered in plots with higher SMV infection rates. Soybean lines with the lowest infection rates had significantly higher yields in 2000 and significantly less seed coat mottling compared with the controls. To our knowledge, this is the first field study demonstrating the effectiveness of pathogen-derived resistance on the temporal and spatial dynamics of pathogen spread in soybean.

Bean pod mottle virus: A Threat to U.S. Soybean Production

“…However, presence of seed coat mottling, as with SMV (9), is unreliable for predicting seedborne BPMV. One report, using a single soybean cultivar, suggests a positive linear relationship between percent mottling and amount of seedborne SMV (84). Recent work with other soybean cultivars demonstrates that either a positive or negative linear relationship between percent seed coat mottling and relative amount of seedborne BPMV can be demonstrated and that the relationship appears to be cultivar dependent in seed lots that were tested and found not to have SMV (J. H. Hill, unpublished).…”

Soybean natural cross-pollination rates under field conditions

“…and Bonpl.] gene (Streit et al, 2001;Townsend and Thomas, 1994;1996) to lines transformed to express virus coat proteins (Reddy et al, 2001;Steinlage et al, 2002;Wang et al, 2001), as well as soybean lines incorporating a Bacillus thuringiensis cry1Ac transgene (Walker et al, 2002). While many of these transformants may have limited commercial potential, it is likely that future cultivars with various transgenes and transgene combinations will be commercially available.…”

Detection, Understanding and Controlof Soybean Mosaic Virus

“…Two transgenic lines showed high resistance to all SMV strains or isolates tested including G2, G6, G7 and an isolate named A15 obtained from South Carolina . None of the transgenic lines showed immunity to SMV infection (Steinlage et al, 2002). Since the transgenes that produce mRNA containing an intron-spanned hairpin structure usually induce high level of PTGS (Smith et al, 2000;Waterhouse et al, 2001), it is expected that soybean transformed with such constructs may obtain stronger resistance or immunity to SMV.…”