Temporal and spatial patterns of transforming growth factor-β1 expression in developing rat molars

D’Souza, Rena N.; Happonen, Risto‐Pekka; Ritter, Nadine; Butler, William T.

doi:10.1016/0003-9969(90)90015-3

Cited by 74 publications

(33 citation statements)

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“…At these stages uPA could be involved in cell invasion and extracellular proteolysis either directly or through activation of plasminogen into plasmin. In addition, uPA could be available for the direct or plasmin-dependent activation of metalloprotease proenzymes (Randall & Hall, 2002) and growth factors known to be involved in tooth development, such as HGF (Naldini et al, 1992;Vigna et al, 1994;Tabata et al, 1996;Thesleff & Sharpe, 1997;Matsumura et al, 1998;Schmitt et al, 2000) TGF-b1 (D'Souza et al, 1990;Vaahtokari et al, 1991;Taipale et al, 1992;Rifkin et al, 1999) or IGF-I (Campbell et al, 1992;Joseph et al, 1994). It is of interest that uPA mRNA was undetectable in ameloblasts yet it was continuously detected in the stratum intermedium, albeit more prominently in the molar teeth.…”

Section: Discussionmentioning

confidence: 97%

Urokinase-type Plasminogen Activator mRNA is Expressed in Normal Developing Teeth and Leads to Abnormal Incisor Enamel in αMUPA Transgenic Mice

et al. 2006

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The urokinase-type plasminogen activator (uPA) is a secreted, inducible serine protease implicated in extracellular proteolysis and tissue remodeling. Here we detected uPA mRNA through in situ hybridization in developing molar and incisor teeth of normal mice at multiple sites of the cap and bell developmental stages. The mRNA was confined to epithelial cells, however, was undetectable in ameloblasts or their progenitor preameloblasts and the inner enamel epithelium. Furthermore, mice of five lines of previously described alphaMUPA transgenic mice, carrying a transgene consisting of the uPA cDNA linked downstream from the alphaA-crystallin promoter, overexpressed uPA mRNA in the same epithelial sites. In addition, alphaMUPA mice showed remarkably high levels of uPA mRNA in ameloblasts, however, exclusively in two specific sites late in incisor development. First, at the late secretory stage, but only on sides of the ameloblast layer. Second, in a limited zone of ameloblasts near the incisal end, coinciding with a striking morphological change of the ameloblast layer and the enamel matrix. In adult alphaMUPA mice, the incisor teeth displayed discoloration and tip fragility, and reduction of the outer enamel as determined by scanning electron microscopy. These results suggest that balanced uPA activity could play a role in normal tooth development. The alphaMUPA tooth phenotype demonstrates a remarkable sensitivity to excessive extracellular proteolysis at the incisor maturation stage of amelogenesis.

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Section: Discussionmentioning

confidence: 97%

Urokinase-type Plasminogen Activator mRNA is Expressed in Normal Developing Teeth and Leads to Abnormal Incisor Enamel in αMUPA Transgenic Mice

et al. 2006

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“…The rest of the dental papilla is not expressing TGF32 (Heikinheimo et al, 1993a). TGFjI-3 are thus all involved in the regulation of tooth development, exhibiting characteristic, partly overlapping expression patterns which suggest unique roles during epithelial-mesenchymal interactions in late tooth development (Lehnert and Akhurst, 1988;Cam et al, 1990;D'Souza et al, 1990;Pelton etal., 1990Pelton etal., , 1991Milanetal., 1991;Wiseand Fan, 1991). Other growth factors, such as FGF1 (aFGF), as well as NGF and IGF and their receptors, are also expressed during stages of odontoblast differentiation (Byers et al, 1990;Cam et al, 1992;Mitsiadis et al, 1992Mitsiadis et al, , 1993Joseph etal., 1993 these cells differentiate into ameloblasts, they no longer express Msx2.…”

Section: Balling Personal Communication) Since Activin Amentioning

confidence: 99%

The Genetic Control of Early Tooth Development

Maas

Bei

1997

Critical Reviews in Oral Biology & Medicine

209

153

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Most vertebrate organs begin their initial formation by a common, developmentally conserved pattern of inductive tissue interactions between two tissues. The developing tooth germ is a prototype for such inductive tissue interactions and provides a powerful experimental system for elucidation of the genetic pathways involved in organogenesis. Members Msxl is required for the transmission of Bmp4 expression from dental epithelium to mesenchyme and also for Lefi expression.In addition, we consider the role of other signaling molecules in the epithelial-mesenchymal interactions leading to tooth formation, the role that transcription factors such as Msx play in the propagation of inductive signals, and the role of extracellular matrix. Last, as a unifying mechanism to explain the disparate tooth phenotypes in Msxl-and Msx2-deficient mice, we propose that later steps in tooth morphogenesis molecularly resemble those in early tooth development. )from a series of instructive and permissive cell-cell interactions (Saxen, 1977a;Wessells, 1977). The determination of the mesoderm and neural plate in vertebrate embryos is referred to as primary induction (Spemann and Mangold, 1924), and is followed by a series of secondary inductive events which further regulate embryonic organ development. Over the last decade, the application of molecular biology to problems in vertebrate development has revealed a considerable amount of information about the nature of the inductive signals which are exchanged between tissue layers during inductive tissue interactions (reviewed in Jessell and Melton, 1992;Kessler and Melton, 1994). This review focuses specifically on the genetic hierarchies which appear to operate during early tooth development, on the nature of the inductive signals which are exchanged between the dental epithelium and mesenchyme, and on the role that one particular class of transcription factors, those belonging to the Msx family of homeobox genes, plays in controlling these inductive tissue interactions. Several excellent recent reviews on tooth development are also available (Ruch, 1995;Sharpe, 1995;Thesleff et al., 1995a Thesleff et al., ,b, 1996 Thesleff and Sahlberg, 1996), and a "tooth gene expression database" is also available on the Internet (http://honeybee.helsinki.fi/toothexp).The dentition not only constitutes a major component of the mammalian craniofacial system, but also provides a powerful and potentially general model for the study of organ development. The possibility of in vitro cultivation, access to cytological and molecular studies, the clear delineation of epithelial and mesenchymal components, the easily distinguished characteristics of ameloblasts and odontoblasts, and the anteroposterior, position-dependent pattern of dentition make the devel-4

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“…Transforming growth factor-␤1 (TGF-␤1) expression has been identified in developing teeth (5), in mature odontoblasts (6), and in human dentin (6,7), indicating a possible role of this factor in the induction of primary dentin formation. TGF-␤1 released from dentin degraded by bacterial or iatrogenic agents might be involved in the immune response of dental pulp to tooth injury (8,9).…”

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confidence: 99%

Behavior of Human Dental Pulp Cells Exposed to Transforming Growth Factor-Beta1 and Acidic Fibroblast Growth Factor in Culture

Luisi¹,

Barbachan²,

Filho³

2007

Journal of Endodontics

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Temporal and spatial patterns of transforming growth factor-β1 expression in developing rat molars

Cited by 74 publications

References 33 publications

Urokinase-type Plasminogen Activator mRNA is Expressed in Normal Developing Teeth and Leads to Abnormal Incisor Enamel in αMUPA Transgenic Mice

Urokinase-type Plasminogen Activator mRNA is Expressed in Normal Developing Teeth and Leads to Abnormal Incisor Enamel in αMUPA Transgenic Mice

The Genetic Control of Early Tooth Development

Behavior of Human Dental Pulp Cells Exposed to Transforming Growth Factor-Beta1 and Acidic Fibroblast Growth Factor in Culture

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