Temporal and spatial expression of connective tissue growth factor (CCN2; CTGF) and transforming growth factor beta type 1 (TGF-beta1) at the utero-placental interface during early pregnancy in the pig

Moussad, E. E.-D. A.; Rageh, M A E; Wilson, Amy K.; Geisert, Rodney D.; Brigstock, David R.

doi:10.1136/mp.55.3.186

Cited by 40 publications

(23 citation statements)

References 49 publications

(34 reference statements)

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“…Both of these PAGs are known to possess proteolytic activity (Telugu & Green 2008, Telugu et al 2010. It seems possible that proteolytically active PAGs present at the interface could process latent growth factors that are known to be present at that location (Munger et al 1998, Rifkin et al 1999, Moussad et al 2002. Indeed, if one assumes that individual PAGs will exhibit distinct substrate specificities, each PAG could be targeting distinct growth factors.…”

Section: Possible Functions Of the Pagsmentioning

confidence: 99%

Placental PAGs: gene origins, expression patterns, and use as markers of pregnancy

et al. 2015

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Pregnancy-associated glycoproteins (PAGs) are abundantly expressed products of the placenta of species within the Cetartiodactyla order (even-toed ungulates). They are restricted to this order and they are particularly numerous in the Bovidae. The PAGs exhibit a range of temporal and spatial expression patterns by the placental trophoblasts and probably represent a group of related proteins that perform a range of distinct functions in the epitheliochorial and synepitheliochorial placental forms. This review presents an overview of the origins of the PAGs, a summary of PAG expression patterns, and their use as markers of pregnancy status. Speculations about their putative role(s) in pregnancy are also presented.Reproduction (2015) 149 R115-R126

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Section: Possible Functions Of the Pagsmentioning

confidence: 99%

Placental PAGs: gene origins, expression patterns, and use as markers of pregnancy

et al. 2015

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“…Ovarian CTGF appears to be involved in recruitment and mitosis of theca cells and maintenance of the corpus luteum (Wandji et al 2000, Slee et al 2001, Liu et al 2002 while uterine CTGF has been implicated in angiogenesis and extracellular matrix remodeling during placentation and decidualization. , Moussad & Brigstock 2000, Uzumcu et al 2000, Moussad et al 2002. Expression of CTGF in the ovary is regulated by TGF-and gonadotrophins , Liu et al 2002 while in the uterus it is regulated by TGF-, estrogen and progesterone (Rageh et al 2001).…”

Section: Introductionmentioning

confidence: 99%

The heparin-binding 10 kDa fragment of connective tissue growth factor (CTGF) containing module 4 alone stimulates cell adhesion

Ball

Rachfal²,

Kemper³

et al. 2003

Journal of Endocrinology

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Connective tissue growth factor (CTGF) is a 349-residue mosaic protein that contains four structural modules implicated in protein-protein interactions. To address the functionality of residues 247-349 (containing module 4 alone), this region of CTGF was produced as a maltose binding protein (MBP) fusion protein in E. coli. After removal of MBP, recombinant CTGF commenced at Glu 247 , was of M r 10 000, was immunoreactive with anti-CTGF [247][248][249][250][251][252][253][254][255][256][257][258][259][260], bound strongly to heparin, and promoted dose-dependent adhesion of fibroblasts, myofibroblasts, endothelial cells, and epithelial cells. An 8 kDa presumptive C-terminally truncated form of CTGF commencing at Glu 247 also promoted cell adhesion. CTGFmediated cell adhesion was abolished by heparin or EDTA. These data demonstrate the presence of heparinbinding and cell-adhesion motifs within the C-terminal 103 residues of CTGF and show that CTGF-mediated cell adhesion is heparin-and divalent cation-dependent. Thus, CTGF isoforms comprising essentially module 4 are intrinsically functional in the absence of the other constituent modules of CTGF.

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“…These fi ndings indicate that CTGF is involved in TGFβ1-enhanced ECM production in SCFs as in other reported cell types. 9,18,23,[26][27][28] To simulate fi broblast migration, we employed an in vitro defect closure produced in a fi broblast monolayer culture. Adding CTGF antisense reduced the percentage of in vitro wound closure.…”

Section: Discussionmentioning

confidence: 99%

“…1,4,7 However, the effects of TGFβ are in part dependent on the secondary induction of other growth factors such as CTGF or platelet-derived growth factor (PDGF). The former factor is involved in ECM accumulation, 17,18 and the latter, in cell proliferation and myofi broblastic conversion by α-smooth muscle actin expression. [19][20][21] Moreover, TGFβ may have a crucial role in the maintenance of homeostasis by activating various cell signaling cascades.…”

Section: Discussionmentioning

confidence: 99%

Connective tissue growth factor modulates extracellular matrix production in human subconjunctival fibroblasts and their proliferation and migration in vitro

et al. 2008

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Temporal and spatial expression of connective tissue growth factor (CCN2; CTGF) and transforming growth factor beta type 1 (TGF-beta1) at the utero-placental interface during early pregnancy in the pig

Cited by 40 publications

References 49 publications

Placental PAGs: gene origins, expression patterns, and use as markers of pregnancy

Placental PAGs: gene origins, expression patterns, and use as markers of pregnancy

The heparin-binding 10 kDa fragment of connective tissue growth factor (CTGF) containing module 4 alone stimulates cell adhesion

Connective tissue growth factor modulates extracellular matrix production in human subconjunctival fibroblasts and their proliferation and migration in vitro

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