Temporal and spatial distribution of insecticide‐resistance mutations in the green peach aphid <scp><i>Myzus persicae</i></scp> (Hemiptera: Aphididae) on primary and secondary host plants in central Chile

Rubio-Meléndez, María E; Sepúlveda, Daniela A.; Ramı́rez, Claudio C.

doi:10.1002/ps.4708

Cited by 9 publications

(11 citation statements)

References 43 publications

(81 reference statements)

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“…Similar patterns have been previously described in Greece (Margaritopoulos et al, 2000(Margaritopoulos et al, , 2002Zitoudi et al, 2001;Poupoulidou et al, 2006;Blackman et al, 2007), Italy (Margaritopoulos et al, 2003), Japan Takada, 2003, 2004;Margaritopoulos et al, 2007aMargaritopoulos et al, , 2007b and the USA (Harlow and Lampert, 1990;Clements et al, 2000aClements et al, , 2000bSrigiriraju et al, 2009), where higher genotype diversity of tobacco aphids has been observed (Zepeda-Paulo et al, 2010). The colour polymorphisms can be explained by the presence of new M. persicae nicotianae genotypes resulting from sexual reproduction events, as reported for M. persicae in Chile on peaches (Rubiano-Rodríguez et al, 2014, 2019Rubio-Meléndez et al, 2018) or by new introduction events from neighbouring countries. These putative new genotypes could possess insecticide resistance mutations that affect tobacco aphid control in tobacco fields in Chile.…”

Section: Introductionsupporting

confidence: 83%

Changes in the genetic composition of Myzus persicae nicotianae populations in Chile and frequency of insecticide resistance mutations

Cabrera-Brandt

Kati

Rubio-Meléndez

et al. 2021

Bull. Entomol. Res.

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Myzus persicae is a cosmopolitan aphid that is highly polyphagous and an important agricultural pest. The subspecies M. persicae nicotianae has been described for highly specialized phenotypes adapted to tobacco (Nicotiana tabacum). In Chile, the population of M. persicae nicotianae was originally composed of a single red genotype that did not possess insecticide resistance mutations. However, in the last decade, variation in the colour of tobacco aphids has been observed in the field. To determine whether this variation stems from the presence of new genotypes, sampling was carried out across the entire distribution of tobacco cultivation regions in Chile. The aphids collected were genotyped, and the frequency of kdr (L1014F), super-kdr (M918T), modification of acetylcholinesterase (MACE) and nicotinic acetylcholine receptor β subunit (nAChRβ) mutations associated with insecticide resistance was determined. A total of 16 new genotypes of M. persicae nicotianae were detected in Chile: four of them possessed the MACE mutation, and none of them possessed the kdr, super-kdr or nAChRβ mutation. The previously described red genotype was not detected in any of the sampled fields over two seasons. These results raise questions about the mechanisms underlying changes in the genetic structure of M. persicae nicotianae populations in Chile. Future research aimed at addressing these questions could provide new insight into aphid evolution and agricultural practices.

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Section: Introductionsupporting

confidence: 83%

Changes in the genetic composition of Myzus persicae nicotianae populations in Chile and frequency of insecticide resistance mutations

Cabrera-Brandt

Kati

Rubio-Meléndez

et al. 2021

Bull. Entomol. Res.

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“…Such a strong linkage between MACE and s‐kdr (M918L) mutations has also been demonstrated in France 21 . Linkage disequilibrium between MACE and kdr and/or s‐kdr (M918T) mutations was also shown in UK, 13 Greece 43 and Chile 50 on some samples from secondary hosts (tobacco, weeds, sweet pepper) but not on samples from peach orchards 43,50 . As aphids from peach consist mostly of sexual genotypes, recombination might break such linkages.…”

Section: Discussionmentioning

confidence: 90%

“…21 Linkage disequilibrium between MACE and kdr and/or s-kdr (M918T) mutations was also shown in UK, 13 Greece 43 and Chile 50 on some samples from secondary hosts (tobacco, weeds, sweet pepper) but not on samples from peach orchards. 43,50 As aphids from peach consist mostly of sexual genotypes, recombination might break such linkages. However, the possibility that the MACE-kdr linkage comes from an interaction between these mutations on fitness cost cannot be ruled out.…”

Section: Discussionmentioning

confidence: 99%

Structuration of multilocus genotypes associated with insecticide resistance of the peach potato aphid,Myzus persicae(Sulzer), in potato fields in southern Belgium

Mingeot

Hautier

Jansen

2020

Pest Management Science

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BACKGROUND: The peach potato aphid, Myzus persicae, has developed resistance to many insecticides. In Belgium, M. persicae is one of the most common aphids in potato fields and one of the most effective virus vectors. We monitored resistance mutations to pyrethroids, carbamates and neonicotinoids and related these results to microsatellite genotyping to provide information to support the choice of management tactics. RESULTS: Most of the 254 aphids tested (97.6%) displayed at least one mutation conferring resistance to pyrethroids (L1014F, M918L and M918T) and 36.2% additionally carried the modified acetylcholinesterase (MACE) carbamates resistance making them resistant to two insecticide action modes. Ten mutation combinations were detected, two of which were frequent and a strong linkage was found between MACE and M918L mutations. The R81T mutation conferring resistance to neonicotinoids was not detected. Microsatellites highlighted a moderate genetic diversity [69 multilocus genotypes (MLG) detected], severe deviations from Hardy-Weinberg expectations, a highly significant excess of heterozygotes and linkage disequilibrium between all pairs of loci. A structuration of MLGs in association with the mutation combinations was observed. Genetic differentiation was mainly not significant between sampling locations and most MLGs were geographically widespread. These results suggest the likely coexistence of parthenogenesis (obligatory or facultative) and sexual reproduction, and the existence of 'old' parthenogenetic overwintering asexual lineages. CONCLUSION: The results of this monitoring at a regional scale provide useful information on insecticide resistance, genetic diversity and reproductive modes, and highlight the need to reduce the insecticide selection pressure and to implement mitigating techniques.

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“…Therefore, numerous insecticides are used to control this insect and in turn, the resistance to multiple insecticides are developed 13 . The most common resistant mechanisms include target-site mutations and over-expression of detoxification enzymes 14 , 15 . In M. persicae , metabolic enzymes reported to confer resistance include esterase E4 (or the mediterranean variant FE4), giving broad-spectrum resistance to organophosphates, carbamates and pyrethroids, and cytochrome P450 CYP6CY3, conferring resistance to neonicotinoids 16 .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Insecticides induced hormesis on the demographic parameters of Myzus persicae and expression changes of metabolic resistance detoxification genes

Sial

Zhao

Zhang

et al. 2018

Sci Rep

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Insecticide induced-hormesis is a bi-phasic phenomenon generally characterized by low-dose induction and high-dose inhibition. It has been linked to insect pest outbreaks and insecticide resistance, which have importance in the integrated pest management (IPM). In this paper, hormesis effects of four insecticides on demographic parameters and expression of genes associated with metabolic resistance were evaluated in a field collected population of the green peach aphid, Myzus persicae Sulzer. The bioassay results showed that imidacloprid was more toxic than acetamiprid, deltamethrin and lambda-cyhalothrin. After exposure to sublethal doses of acetamiprid and imidacloprid for four generations, significant prolonged nymphal duration and increased fecundity were observed. Subsequently, mean generation time (T) and gross reproductive rate (GRR) was significantly increased. Moreover, expression of CYP6CY3 gene associated with resistance to neonicotinoids was increased significantly compared to the control. For pyrethriods, across generation exposure to sublethal doses of lambda cyhalothrin and deltamethrin prolonged the immature development duration. However, the expression of E4 gene in M. persicase was decreased by deltamethrin exposure but increased by lambda cyhalothrin. Based on results, demographic fitness parameters were effected by hormetic dose and accompanied with detoxifying genes alteration, hence, which would be evaluated in developing optimized insect pest management strategies.

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Temporal and spatial distribution of insecticide‐resistance mutations in the green peach aphid Myzus persicae (Hemiptera: Aphididae) on primary and secondary host plants in central Chile

Abstract: This study provides farm-scale evidence that weeds are not an important reservoir of the R allele, but they are for the S allele. Hence, the presence of heterozygous genotypes on peach trees is most likely attributable to weeds. © 2017 Society of Chemical Industry.

Cited by 9 publications

References 43 publications

Changes in the genetic composition of Myzus persicae nicotianae populations in Chile and frequency of insecticide resistance mutations

Changes in the genetic composition of Myzus persicae nicotianae populations in Chile and frequency of insecticide resistance mutations

Structuration of multilocus genotypes associated with insecticide resistance of the peach potato aphid,Myzus persicae(Sulzer), in potato fields in southern Belgium

Evaluation of Insecticides induced hormesis on the demographic parameters of Myzus persicae and expression changes of metabolic resistance detoxification genes

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