Temporal and Spatial Distribution of Auxin Response Factor Genes During Tomato Flower Abscission

Guan, Xiaoxi; Xu, Tongyu; Gao, Song; Qi, Mingfang; Wang, Yanling; Liu, Xin; Li, Tianlai

doi:10.1007/s00344-013-9377-x

Cited by 26 publications

(37 citation statements)

References 36 publications

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“…By expression analysis and genetic mutation, several researches demonstrated that ARF genes could regulate plant organ abscission (Ellis et al 2005;Kuang et al 2012;Guan et al 2014). Abebie et al (2008) reported that the floret abscission in cestrum cut flower was associated with the expression level of AUX/IAA genes, which was consistent with the results reported by Meir et al (2010).…”

Section: Introductionsupporting

confidence: 85%

“…Therefore, based on the orthologous relationship, we could roughly predict biological functions of some CitARF proteins. For instance, AtARF2 were induced by senescence, and could regulate senescence and abscission of floral organs in Arabidopsis (Ellis et al 2005;Guan et al 2014), thereby indicating its ortholog, CitARF10, might be related to tissue senescence and abscission. However, in different tissues and during fruitlet abscission, CitARF10 exhibited constitutive expression, which suggested that its function may be more diverse than that of AtARF2.…”

Section: Discussionmentioning

confidence: 98%

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The ARF, AUX/IAA and GH3 gene families in citrus: genome-wide identification and expression analysis during fruitlet drop from abscission zone A

Xie

Pang

et al. 2015

Mol Genet Genomics

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Completion of the whole genome sequencing of citrus enabled us to perform genome-wide identification and functional analysis of the gene families involved in agronomic traits and morphological diversity of citrus. In this study, 22 CitARF, 11 CitGH3 and 26 CitAUX/IAA genes were identified in citrus, respectively. Phylogenetic analysis revealed that all the genes of each gene family could be subdivided into three groups and showed strong evolutionary conservation. The GH3 and AUX/IAA gene families shrank and ARF gene family was highly conserved in the citrus genome after speciation from Arabidopsis thaliana. Tissue-specific expression profiles revealed that 54 genes were expressed in at least one tissue while just 5 genes including CitARF07, CitARF20, CitGH3.04, CitAUX/IAA25 and CitAUX/IAA26 with very low expression level in all tissues tested, suggesting that the CitARF, CitGH3 and CitAUX/IAA gene families played important roles in the development of citrus organs. In addition, our data found that the expression of 2 CitARF, 4 CitGH3 and 4 AUX/IAA genes was affected by IAA treatment, and 7 genes including, CitGH3.04, CitGH3.07, CitAUX/IAA03, CitAUX/IAA04, CitAUX/IAA18, CitAUX/IAA19 and CitAUX/IAA23 were related to fruitlet abscission. This study provides a foundation for future studies on elucidating the precise role of citrus ARF, GH3 and AUX/IAA genes in early steps of auxin signal transduction and open up a new opportunity to uncover the molecular mechanism underlying citrus fruitlet abscission.

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Section: Introductionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 98%

The ARF, AUX/IAA and GH3 gene families in citrus: genome-wide identification and expression analysis during fruitlet drop from abscission zone A

Xie

Pang

et al. 2015

Mol Genet Genomics

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“…In tomato abscission, ARF 8-1, ARF14 and ARF17 play an important role in the early stage; auxin may mediate the expression of ARF 8-1 and ARF14, while ethylene may mediate ARF13-1. Previous studies have shown that miR160 targets ARF10, ARF16 and ARF17, while miR167 targets ARF8, ARF8-1 and ARF6, indicating that miRNAs are involved in mediating ARF gene expression (Guan et al 2014). In tomato abscission, expression of miR393 remained stable for the first 4 h, while ethylene up-regulated its expression.…”

Section: Expression Profiles Of Mirnas and Targets During Pedicel Absmentioning

confidence: 95%

Small RNA and degradome sequencing reveals microRNAs and their targets involved in tomato pedicel abscission

Wang

Liu

et al. 2015

Planta

Self Cite

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We constructed small RNA and degradome sequencing libraries to identify miRNAs and targets involved in tomato pedicel abscission, and confirmed their roles via quantitative real-time PCR. MicroRNAs (miRNAs) are endogenous small RNAs which play crucial negatively regulatory roles at both the transcriptional and post-transcriptional levels in plants; however, limited knowledge is available on the expression profiles of miRNAs and their target genes during tomato pedicel abscission. Taking advantage of small RNA (sRNA) and degradome sequencing technology, a total of 56 known and 11 novel candidate miRNAs targeting 223 mRNA genes were confirmed during pedicel abscission. Gene ontology annotation and KEGG pathway analysis showed that these target genes were significantly enriched in intracellular, membrane-bounded organelle-related biological processes as well as in metabolic, plant-pathogen interaction and hormone signaling pathways. We screened 17 miRNA/target pairs for further analysis and performed quantitative real-time PCR to identify the roles. Cluster analysis of selected miRNAs revealed that the expression profiles of miRNAs varied in different stages of abscission and could be impacted by ethylene treatment. In the present study, the correlations between miRNAs and targets suggested a complex regulatory network of miRNA-mediated target interaction during pedicel abscission. Additionally, the expression profiles of miRNAs and their targets changed by ethylene might be a considerable reason why ethylene promotes pedicel abscission. Our study provides new insights into the expression and regulatory profiles of miRNAs during tomato pedicel abscission.

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“…On the other hand, IAA application immediately after flower removal inhibited all the cascade of abscission events and the changes in the expression of auxin-induced or auxin-repressed genes [74**]. Two later studies showed a rapid decrease of Aux/IAA [191] and ARF [70] genes after tomato flower removal. Additionally, quantitative and DR5::GUS data demonstrated a fast IAA depletion after flower removal [70].…”

Section: Elucidation Of the Sequence Of Events Leading To Organ Abscimentioning

confidence: 99%

Role of auxin depletion in abscission control

2015

Stewart Postharvest Review

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Purpose of review:Abscission is a programmed developmental process initiated by auxin depletion. This review summarizes the mechanisms leading to auxin depletion in the abscission zone (AZ), evaluates the methods for estimation of the spatio-temporal auxin levels, demonstrates how auxin depletion occurs during natural, stressinduced, and artificially-induced organ abscission, and presents new evidence for early and late events resulting from auxin depletion which lead to organ abscission. Findings: Auxin depletion occurs during natural developmental processes which end in organ abscission (leaf and flower senescence, fruit ripening, and self-pruning) and stress-induced abscission, and following artificial organ removal in the tomato model system. Stress-induced auxin depletion is mediated by increased ethylene and reactive oxygen species (ROS) production and carbohydrate starvation. Similar changes in auxin-related genes occurred in both flower AZ (FAZ) and leaf AZ (LAZ) following flower or leaf removal, respectively, suggesting a similar regulation of the abscission process of these organs. Auxin depletion resulted from decreased indole-3acetic acid (IAA) biosynthesis and transport, as well as from enhanced IAA transport autoinhibition (ATA), conjugation and oxidative IAA catabolism. Functional analyses of several target genes delaying abscission, such as Knotted-Like Homeobox Protein1 (KD1), Tomato Proline Rich Protein (TPRP), Ethylene Responsive Factor52 (ERF52), and Ribonuclease LX (LX), shed light on various events operating in response to auxin depletion in tomato FAZ and/or LAZ. The information gained allows a better understanding of the abscission process driven by auxin depletion, and might lead to development of improved methods for abscission control in horticultural crops. Direction for future research: A better understanding of abscission regulation as it pertains to auxin depletion will require advanced molecular tools such as microarrays, new generation sequencing (NGS), transcriptomic, functional, and proteomic analyses of target genes and proteins found to operate in the abscission process.

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Temporal and Spatial Distribution of Auxin Response Factor Genes During Tomato Flower Abscission

Cited by 26 publications

References 36 publications

The ARF, AUX/IAA and GH3 gene families in citrus: genome-wide identification and expression analysis during fruitlet drop from abscission zone A

The ARF, AUX/IAA and GH3 gene families in citrus: genome-wide identification and expression analysis during fruitlet drop from abscission zone A

Small RNA and degradome sequencing reveals microRNAs and their targets involved in tomato pedicel abscission

Role of auxin depletion in abscission control

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