Temporal and spatial control of gene expression in horticultural crops

Dutt, Manjul; Dhekney, Sadanand A.; Soriano, Leonardo; Kandel, R.; Grosser, Jude W.

doi:10.1038/hortres.2014.47

Cited by 97 publications

(72 citation statements)

References 361 publications

(285 reference statements)

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“…This result is probably due to the large pool size of PA polymers in seed coats; thus, the effect of the reduction caused by KFB CHS is less pronounced. Alternatively, the observed weak effect of KFB CHS expression on PA accumulation may be just due to the less efficient expression of the 35S promoter in the seed coat, as previously reported (Chen et al, 2014;Dutt et al, 2014), where PA is synthesized locally.…”

Section: Manipulating Kfb Chs Expression In Arabidopsis Disturbs Flavmentioning

confidence: 88%

A Proteolytic Regulator Controlling Chalcone Synthase Stability and Flavonoid Biosynthesis in Arabidopsis

et al. 2017

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Flavonoids represent a large family of specialized metabolites involved in plant growth, development, and adaptation. Chalcone synthase (CHS) catalyzes the first step of flavonoid biosynthesis by directing carbon flux from general phenylpropanoid metabolism to flavonoid pathway. Despite extensive characterization of its function and transcriptional regulation, the molecular basis governing its posttranslational modification is enigmatic. Here, we report the discovery of a proteolytic regulator of CHS, namely, KFB CHS , a Kelch domain-containing F-box protein in Arabidopsis thaliana. KFB CHS physically interacts with CHS and specifically mediates its ubiquitination and degradation. KFB CHS exhibits developmental expression patterns in Arabidopsis leaves, stems, and siliques and strongly responds to the dark-to-light (or the light-to-dark) switch, the blue, red, and far-red light signals, and UV-B irradiation. Alteration of KFB CHS expression negatively correlates to the cellular concentration of CHS and the production of flavonoids. Our study suggests that KFB CHS serves as a crucial negative regulator, via mediating CHS degradation, coordinately controlling flavonoid biosynthesis in response to the developmental cues and environmental stimuli.

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Section: Manipulating Kfb Chs Expression In Arabidopsis Disturbs Flavmentioning

confidence: 88%

A Proteolytic Regulator Controlling Chalcone Synthase Stability and Flavonoid Biosynthesis in Arabidopsis

et al. 2017

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“…The plastid vector for this transformation contains a Cry1Ac gene driven by a 35S promoter that enables the production of the Cry1Ac protein in almost all parts of the plant (Perlak et al, 2001). The 35S promoter is by far the most widely used promoter in plant transformation, especially for dicotyledonous plant species, including cotton (Dutt et al, 2014;Porto et al, 2014). When transferred to plants, this promoter is capable of driving high levels of gene expression in most plant tissues/organs.…”

Section: Discussionmentioning

confidence: 99%

Transgenic Bt cotton driven by the green tissue-specific promoter shows strong toxicity to lepidopteran pests and lower Bt toxin accumulation in seeds

Wang

Zhu

Liang

et al. 2016

Sci. China Life Sci.

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A promoter of the PNZIP (Pharbitis nil leucine zipper) gene (1.459 kb) was cloned from Pharbitis nil and fused to the GUS(-glucuronidase) and Bacillus thuringiensis endotoxin (Cry9C) genes. Several transgenic PNZIP::GUS and PNZIP::Cry9C cotton lines were developed by Agrobacterium-mediated transformation. Strong GUS staining was detected in the green tissues of the transgenic PNZIP::GUS cotton plants. In contrast, GUS staining in the reproductive structures such as petals, anther, and immature seeds of PNZIP::GUS cotton was very faint. Two transgenic PNZIP::Cry9C lines and one transgenic cauliflower mosaic virus (CaMV) 35S::Cry9C line were selected for enzyme-linked immunosorbent assay (ELISA) and insect bioassays. Expression of the Cry9C protein in the 35S::Cry9C line maintained a high level in most tissues ranging from 24.6 to 45.5 μg g 1 fresh weight. In green tissues such as the leaves, boll rinds, and bracts of the PNZIP::Cry9C line, the Cry9C protein accumulated up to 50.2, 39.7, and 48.3 µg g 1 fresh weight respectively. In contrast, seeds of the PNZIP::Cry9C line (PZ1.3) accumulated only 0.26 µg g 1 fresh weight of the Cry9C protein, which was 100 times lower than that recorded for the seeds of the CaMV 35S::Cry9C line. The insect bioassay showed that the transgenic PNZIP::Cry9C cotton plant exhibited strong resistance to both the cotton bollworm and the pink bollworm. The PNZIP promoter could effectively drive Bt toxin expression in green tissues of cotton and lower accumulated levels of the Bt protein in seeds. These features should allay public concerns about the safety of transgenic foods. We propose the future utility of PNZIP as an economical, environmentally friendly promoter in cotton biotechnology. tissue-specific promoter, GUS expression, Bt toxin, transgenic cotton plants Citation:Wang, Q., Zhu, Y., Sun, L., Li, L., Jin, S., and Zhang, X. (2016). Transgenic Bt cotton driven by the green tissue-specific promoter shows strong toxicity to lepidopteran pests and lower Bt toxin accumulation in seeds. Sci China Life Sci 59, 172 -182.

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“…Together with a large number of available promoters (Dutt et al 2014), it could provide an additional tool for genetic engineering especially of wood properties. In the past, the application of a xylem specific promoter of cinnamate 4-hydroxylase (C4H) promoter to control the expression of an Arabidopsis ferulate 5-hydroxylase (F5H) gene led to significantly increase lignin content in transgenic hybrid poplar trees (Populus tremula x P. alba) (Stewart et al 2009).…”

Section: Molecular Cloning and Nucleotide Sequence Analysis Of The Eumentioning

confidence: 99%

Isolation and characterization of the 4-coumarate:coenzyme A ligase (4CL1) promoter from Eucalyptus camaldulensis

Hue

Hải

et al. 2016

Physiol Mol Biol Plants

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The most important enzyme of the phenylpropanoid pathway, 4-coumarate:coenzyme A ligase (4CL), is encoded by several homologous genes including 4CL1. The 4CL1 promoter is a tissue-specific gene expression element, particularly active in the secondary xylem or older stems. In this study, the 1127 bp 5 0 -upstream region of the 4CL1 coding sequence from Eucalyptus camaldulensis, Euc4CL1, was isolated and characterized. Essential putative cis-elements in the Euc4CL1 promoter included: a TATA-box at -22/-28 position, two CCAAT-boxes at -256/-260 and -277/-281 positions, respectively, an AC-element at -328/-336 and A-boxes at -115/-120 and -990/-995 positions. To investigate the effect of the Euc4CL1 promoter on gene expression, a plant transformation vector, pEuc4CL1p, containing the reporter gene for b-glucuronidase (GUS) under the control of Euc4CL1 promoter was constructed based on the pBI101 backbone and introduced in tobacco plants. Stable expression of the GUS gene in transgenic lines was analysed by a histochemical GUS assay. The results indicated the specific expression of the GUS gene in the stem xylem cells of transgenic tobacco lines was controlled by the Euc4CL1 promoter. The observations suggest the isolated Euc4CL1 promoter is a potential candidate for driving the expression of a foreign gene in plant xylem tissues.

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Temporal and spatial control of gene expression in horticultural crops

Cited by 97 publications

References 361 publications

A Proteolytic Regulator Controlling Chalcone Synthase Stability and Flavonoid Biosynthesis in Arabidopsis

A Proteolytic Regulator Controlling Chalcone Synthase Stability and Flavonoid Biosynthesis in Arabidopsis

Transgenic Bt cotton driven by the green tissue-specific promoter shows strong toxicity to lepidopteran pests and lower Bt toxin accumulation in seeds

Isolation and characterization of the 4-coumarate:coenzyme A ligase (4CL1) promoter from Eucalyptus camaldulensis

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