Temporal and morphologic characteristics of pinopod expression across the secretory phase of the endometrial cycle in normally cycling women with proven fertility

Usadi, Rebecca; Murray, Michael J.; Bagnell, Robert; Fritz, Marc A.; Kowalik, Ania; Meyer, William R.; Lessey, Bruce A.

doi:10.1016/s0015-0282(02)04929-4

Cited by 71 publications

(49 citation statements)

References 30 publications

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“…The primate uterus is unique in that complete membrane transformation takes place in every cycle, regardless of conception. Membrane transformation in the cycling human uterus is both regionally and temporally restricted (Nikas, 1999;Usadi et al, 2003). The same regional and temporal restrictions do not exist in Old World monkeys.…”

Section: Discussionmentioning

confidence: 95%

Compartmental distinctions in uterine Muc-1 expression during early pregnancy in cynomolgous macaque (Macaca fascicularis) and baboon (Papio anubis)

Julian¹,

Enders²,

Fazleabas³

et al. 2005

Human Reproduction

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BACKGROUND: Loss of the transmembrane mucin, Muc-1, is a molecular correlate of the acquisition of uterine receptivity to embryo adhesion in most species examined. In macaques, two distinct adhesion events occur at opposite sides of the uterus. Attachment to the secondary site is delayed relative to the primary site. The aim was to determine if Muc-1 is removed at secondary sites prior to trophoblast attachment. METHODS: We examined Muc-1 expression in the uteri of cynomolgus macaque and baboon during early implantation by immunocytochemistry. RESULTS: Luminal epithelia were devoid of Muc-1 at all stages examined at both primary and secondary adhesion sites. Loss of Muc-1 in luminal epithelia was found to be maternally determined, accompanied membrane transformation in both macaque and baboon, and at secondary implantation sites, preceded trophoblast attachment. In contrast, glandular epithelia in pregnant macaques exhibited a temporal and compartmentalized gradient of Muc-1 loss confined to the implantation sites. Glandular epithelia in the pregnant baboon uterus were uniformly negative for Muc-1. CONCLUSIONS: Restriction of the Muc-1 loss in glandular epithelia to conceptual cycles may reflect the fundamental distinctions among epithelia of the various uterine compartments and the differential modulation of Muc-1 that occurs within these compartments in conceptual and non-conceptual cycles.

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Section: Discussionmentioning

confidence: 95%

Compartmental distinctions in uterine Muc-1 expression during early pregnancy in cynomolgous macaque (Macaca fascicularis) and baboon (Papio anubis)

Julian¹,

Enders²,

Fazleabas³

et al. 2005

Human Reproduction

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“…NCSs may be a hallmark of receptive endometrium because they define a luteal window that closely mirrors serum progesterone levels. By contrast, pinopodes -apical membrane protrusions thought to be critical for and present at the site of blastocyst attachment -persist through early menses and pregnancy (Acosta et al, 2000;Bentin-Ley et al, 1999;Nikas et al, 1995;Usadi et al, 2003). Additionally, the value of pinopodes as implantation markers has recently been questioned (Petersen et al, 2005;Quinn et al, 2007).…”

Section: Fig 4 the Ncs Marks The Implantation Windowmentioning

confidence: 99%

Nuclear pore complex proteins mark the implantation window in human endometrium

Guffanti

Kittur

Brodt

et al. 2008

Journal of Cell Science

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Nucleolar channel systems (NCSs) are membranous organelles appearing transiently in the epithelial cell nuclei of postovulatory human endometrium. Their characterization and use as markers for a healthy receptive endometrium have been limited because they are only identifiable by electron microscopy. Here we describe the light microscopic detection of NCSs using immunofluorescence. Specifically, the monoclonal nuclear pore complex antibody 414 shows that NCSs are present in about half of all human endometrial epithelial cells but not in any other cell type, tissue or species. Most nuclei contain only a single NCS of uniform 1 μm diameter indicating a tightly controlled organelle. The composition of NCSs is as unique as their structure; they contain only a subset each of the proteins of nuclear pore complexes, inner nuclear membrane, nuclear lamina and endoplasmic reticulum. Validation of our robust NCS detection method on 95 endometrial biopsies defines a 6-day window, days 19-24 (±1) of an idealized 28 day cycle, wherein NCSs occur. Therefore, NCSs precede and overlap with the implantation window and serve as potential markers of uterine receptivity. The immunodetection assay, combined with the hitherto underappreciated prevalence of NCSs, now enables simple screening and further molecular and functional dissection.

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“…However, the projections found late in the cycle are known to differ in shape from pinopods, due to probably apoptotic/necrotic processes that precede menstruation. 111 In contrast to the above studies, Usadi et al 112 postulated that pinopods are observed first on luteal day 5, corresponding with the onset of mid-luteal phase increase in serum progesterone levels and persist for the entire duration of the secretory phase. Interestingly, they observed that the morphology of pinopods changes as the secretory phase advances and have suggested that the morphology of pinopods, rather than their mere presence or absence, appears useful as a marker of endometrial receptivity.…”

Section: Microarchitecturementioning

confidence: 93%

Endometrial receptivity: Clinical assessment in relation to fertility, infertility, and antifertility

Makker

Singh

2006

Med. Res. Rev.

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Fertility in humans and other mammalian species depends absolutely on synchronous events that render the developing blastocyst and the receiving uterus competent for implantation. Endometrial receptivity is defined as the period during which the endometrial epithelium acquires functional, but transient, ovarian steroid-dependent status supportive to blastocyst acceptance and implantation. Once inside the uterus, the blastocyst is surrounded by an intact luminal epithelium, which is considered to act as barrier to its attachment, except for this short period of high endometrial receptivity to blastocyst signal(s). Its transport and permeability properties, in conjunction with cellular action of the endometrium and the embryo, have been suggested to influence creation and maintenance of informational and nutritional status of uterine luminal milieu. This period, also termed as the 'window of implantation,' is limited to days 20-24 of menstrual cycle in humans. However, establishment of endometrial receptivity is still a biological mystery that remains unsolved despite marked advances in our understanding of endometrial physiology following extensive research associated with its development and function. This review deals with various structural, biochemical, and molecular events in the endometrium coordinated within the implantation window that constitute essential elements in the repertoire that signifies endometrial receptivity and is aimed to achieve a better understanding of its relationship to fertility, infertility, and for the development of targeted antifertility agents for human use and welfare.

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Temporal and morphologic characteristics of pinopod expression across the secretory phase of the endometrial cycle in normally cycling women with proven fertility

Cited by 71 publications

References 30 publications

Compartmental distinctions in uterine Muc-1 expression during early pregnancy in cynomolgous macaque (Macaca fascicularis) and baboon (Papio anubis)

Compartmental distinctions in uterine Muc-1 expression during early pregnancy in cynomolgous macaque (Macaca fascicularis) and baboon (Papio anubis)

Nuclear pore complex proteins mark the implantation window in human endometrium

Endometrial receptivity: Clinical assessment in relation to fertility, infertility, and antifertility

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