Temporal analysis of the interference caused by paratuberculosis vaccination on the tuberculosis diagnostic tests in goats

Roy, Álvaro; Infantes-Lorenzo, José Antonio; Blázquez, José; Venteo, Ángel; Mayoral, Francisco José; Domínguez, Mercedes; Moreno, Inmaculada; Romero, Beatriz; Juan, Lucía de; Grau, Anna; Domı́nguez, Lucas; Bezos, Javier

doi:10.1016/j.prevetmed.2018.05.010

Cited by 16 publications

(18 citation statements)

References 32 publications

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“…Similar diagnostic interferences have been recorded regarding MAP diagnosis in goats [30, 31]. Our results support the view that vaccination against MAP strongly impacts on ELISA Sp since significant lower Sp was observed in vaccinated goats [29, 32, 33].…”

Section: Discussionsupporting

confidence: 90%

Specificity of serological test for detection of tuberculosis in cattle, goats, sheep and pigs under different epidemiological situations

Infantes-Lorenzo

Moreno

Roy³

et al. 2019

BMC Vet Res

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Background Serum antibody detection has potential as a complementary diagnostic tool in animal tuberculosis (TB) control, particularly in multi-host systems. The objective of the present study was to assess the specificity (Sp) of an enzyme-linked immunosorbent assay (ELISA) based on the new multiprotein complex P22 for the detection of specific antibodies against the Mycobacterium tuberculosis complex (MTC) in the four most relevant domestic animals acting as MTC hosts: cattle, goat, sheep and pig. We used sera from an officially TB-free (OTF) country, Norway, and from a non-OTF one, Spain. The samples included sera from goats that had been vaccinated against M. avium subsp. paratuberculosis (MAP) and sheep from a herd in which Corynebacterium pseudotuberculosis had been isolated. Results In cattle, the Sp ranged from 92.5 (IC95% 90.7–94) to 99.4% (IC95% 98.3–99.8) depending on the cut-off used and the origin of the samples (Spain or Norway). Sp in cattle (cut-off point 100) was significantly higher ( P < 0.05) for Norwegian samples. By contrast, Sp in goats was consistently low at the 100 cut-off [30.9 (CI95%23.4–39.5)-78% (CI95% 68.9–85)]. A higher cut-off of 150 improved Sp in Norwegian goats [97% (CI95% 91.6–99)], but still yielded a poor Sp of 56.1% (CI95% 47.3–64.6) in Spanish goats. In Norway at the 100 cut-off the Sp was 58.3 (CI95% 42.2–72.9) and 90.6% (CI95% 81–95.6) in MAP vaccinated and non-vaccinated goats, respectively, indicating interference due to MAP vaccination. Sp in sheep was between 94.4 (CI95% 91.7–96.3) and 100% (CI95% 96.3–100) depending on the cut-off and country, and no diagnostic interference due to infection with C. pseudotuberculosis was recorded. Sp in pigs was 100%, regardless the cut-off point applied, and no significant differences were observed between pigs from Norway and from Spain. Conclusions Due to its excellent Sp in pigs and acceptable Sp in cattle and sheep, this ELISA may constitute a suitable option for TB screening at herd level, particularly in OTF-countries.

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Section: Discussionsupporting

confidence: 90%

Specificity of serological test for detection of tuberculosis in cattle, goats, sheep and pigs under different epidemiological situations

Infantes-Lorenzo

Moreno

Roy³

et al. 2019

BMC Vet Res

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“…In conjunction with the vaccine effects, decreasing shedding and incidence of clinical cases such management systems will enable better control of caprine paratuberculosis. Similar to Roy et al [33], no false positive bovine PPD reactors in the Bovigam test in the TB-free adult goats a year or longer after MAP vaccination were observed, which is important when considering the monitoring of TB infections.…”

Section: Discussionsupporting

confidence: 78%

Effects of Age and Environment on Adaptive Immune Responses to Mycobacterium avium subsp. paratuberculosis (MAP) Vaccination in Dairy Goats in Relation to Paratuberculosis Control Strategies

Koets

Ravesloot

Ruuls

et al. 2019

Veterinary Sciences

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Paratuberculosis infection is caused by Mycobacterium avium subsp. paratuberculosis (MAP). In the Netherlands, 75% herd level prevalence of caprine paratuberculosis has been estimated, and vaccination is the principal control strategy applied. Most goat dairy farms with endemic paratuberculosis systematically vaccinate goat kids in the first months of life with a commercially available whole cell MAP vaccine. We hypothesized that the development of adaptive immune responses in goats vaccinated at young age depends on the environment they are raised in, and this has implications for the application of immune diagnostic tests in vaccinated dairy goats. We evaluated the early immune response to vaccination in young goat kids sourced from a MAP unsuspected non-vaccinated herd and raised in a MAP-free environment. Subsequently we compared these with responses observed in birth year and vaccination matched adult goats raised on farms with endemic paratuberculosis. Results indicated that initial adaptive immune responses to vaccination are limited in a MAP-free environment. In addition, adult antibody positive vaccinated goats raised in a MAP endemic environment are less likely to be IS900 PCR-positive as compared to antibody negative herd mates. We conclude that test-and-cull strategies in a vaccinated herd are currently not feasible using available immune diagnostic tests.

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“…However, a slight loss of Sp in the P22-IGRAs was detected compared to STAND-IGRA. Even so, the Sp was within ranges (95–100%) described for STAND-IGRA in previous studies ( 11 , 38 , 45 ). This mild reduction in Sp could be explained by the high concentration of P22 used for stimulation of whole blood (20 μg/ml) and by the fact that P22 complex contains 21 proteins also present in M. avium ( 23 ), which can cause cross-reactivity with MAP vaccination and/or infection.…”

Section: Discussionsupporting

confidence: 77%

“…These findings indicate that some degree of cross-reactivity due to MAP vaccination was still maintained. Similarly, interferences of MAP vaccination on TB diagnosis, mainly in CMI-based diagnostic tests, were previously observed in MAP-vaccinated goats ( 14 , 45 ).…”

Section: Discussionsupporting

confidence: 64%

“…This mild reduction in Sp could be explained by the high concentration of P22 used for stimulation of whole blood (20 μg/ml) and by the fact that P22 complex contains 21 proteins also present in M. avium ( 23 ), which can cause cross-reactivity with MAP vaccination and/or infection. Indeed, the interference of MAP vaccination on STAND-IGRA has been previously observed in adult MAP-vaccinated goats ( 13 , 14 , 45 ). The Sp of P22-IGRAs considerably decreased in BCG-vaccinated herds compared to that previously described for the STAND-IGRA ( 16 ).…”

Section: Discussionmentioning

confidence: 73%

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Evaluation of P22 Antigenic Complex for the Immuno-Diagnosis of Tuberculosis in BCG Vaccinated and Unvaccinated Goats

et al. 2020

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Temporal analysis of the interference caused by paratuberculosis vaccination on the tuberculosis diagnostic tests in goats

Cited by 16 publications

References 32 publications

Specificity of serological test for detection of tuberculosis in cattle, goats, sheep and pigs under different epidemiological situations

Specificity of serological test for detection of tuberculosis in cattle, goats, sheep and pigs under different epidemiological situations

Effects of Age and Environment on Adaptive Immune Responses to Mycobacterium avium subsp. paratuberculosis (MAP) Vaccination in Dairy Goats in Relation to Paratuberculosis Control Strategies

Evaluation of P22 Antigenic Complex for the Immuno-Diagnosis of Tuberculosis in BCG Vaccinated and Unvaccinated Goats

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