Template-assisted nano-patterning of solid surfaces

Boeckl, Maximiliane S.; Baas, Tracey; Fujita, Akio; Hwang, Ki Oh; Bramblett, Ariana L.; Ratner, Buddy D.; Rogers, James W.; Sasaki, Tokio

doi:10.1002/(sici)1097-0282(1998)47:2<185::aid-bip8>3.0.co;2-q

Cited by 19 publications

(14 citation statements)

References 14 publications

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“…The adsorbed protein layer can affect device performance and longevity by a variety of means such as bacterial infection, − the formation of calcium-containing deposits, device toxicity, tissue integration, and animal cell adhesion. − For example, plasma proteins such as collagen, fibrinogen, fibronectin, and vitronectin have been related to bacterial adhesion and growth in long-term biomaterial implants. Characterization of the adsorbed protein film provides important information for the rational design of biomaterial surfaces that resist protein adsorption − or specifically adsorb proteins of interest. − …”

Section: Introductionmentioning

confidence: 99%

Characterization of Adsorbed Protein Films by Time-of-Flight Secondary Ion Mass Spectrometry with Principal Component Analysis

2001

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Characterization of the adsorbed protein film that forms upon implantation of a biomedical device is a long-standing interest in biomaterials research. Time-of-flight secondary ion mass spectrometry (ToF−SIMS) is a powerful method for the characterization of adsorbed proteins on biomaterial surfaces due to its chemical specificity and surface sensitivity. However, the SIMS fragmentation patterns for proteins are quite complex due to the heterogeneity of the protein sequence. Therefore, the multivariate analysis technique principal components analysis (PCA) was used to obtain a more detailed interpretation of the protein SIMS spectra. This study utilizes single component adsorbed protein films on three model substrates and multivariate analysis of the ToF−SIMS data to determine the identity of protein films. Furthermore, ToF−SIMS and PCA were used to give insight into the composition of a 1% bovine plasma protein film. The single component spectra from 13 different proteins were readily distinguishable using PCA. The major component of the 1% bovine plasma film was found to shift from fibrinogen to γ-globulins over the course of 2 h, in agreement with the current literature. This study shows how combination of ToF−SIMS and PCA provides new insights into the composition of adsorbed protein films on biomaterial surfaces.

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Section: Introductionmentioning

confidence: 99%

Characterization of Adsorbed Protein Films by Time-of-Flight Secondary Ion Mass Spectrometry with Principal Component Analysis

2001

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“…For the purpose of improving the cell–biomaterial interface, peptides and ECM proteins have been immobilized onto solid substrates such as glass, silicon micromachined probes, and glassy carbon electrodes. Various methods have been used, such as electron spinning, covalent bonding, chemisorption, self‐assembling monolayers (SAM), microcontact printing, and electrochemical polymerization 4, 5, 21–25…”

Section: Introductionmentioning

confidence: 99%

“…Various methods have been used, such as electron spinning, covalent bonding, chemisorption, self-assembling monolayers (SAM), microcontact printing, and electrochemical polymerization. 4,5,[21][22][23][24][25] We believe that electrochemical polymerization has many advantages over other methods in the surface modification of bioelectronic devices. The method can be used on many different electrically conductive surfaces whereas chemical attachment techniques usually are limited to a few substrate choices.…”

Section: Introductionmentioning

confidence: 99%

Surface modification of neural recording electrodes with conducting polymer/biomolecule blends

Cui

Lee

Raphael

et al. 2001

J. Biomed. Mater. Res.

489

417

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The interface between micromachined neural microelectrodes and neural tissue plays an important role in chronic in vivo recording. Electrochemical polymerization was used to optimize the surface of the metal electrode sites. Electrically conductive polymers (polypyrrole) combined with biomolecules having cell adhesion functionality were deposited with great precision onto microelectrode sites of neural probes. The biomolecules used were a silk-like polymer having fibronectin fragments (SLPF) and nonapeptide CDPGYIGSR. The existence of protein polymers and peptides in the coatings was confirmed by reflective microfocusing Fourier transform infrared spectroscopy (FTIR). The morphology of the coating was rough and fuzzy, providing a high density of bioactive sites for interaction with neural cells. This high interfacial area also helped to lower the impedance of the electrode site and, consequently, to improve the signal transport. Impedance spectroscopy showed a lowered magnitude and phase of impedance around the biologically relevant frequency of 1 kHz. Cyclic voltammetry demonstrated the intrinsic redox reaction of the doped polypyrrole and the increased charge capacity of the coated electrodes. Rat glial cells and human neuroblastoma cells were seeded and cultured on neural probes with coated and uncoated electrodes. Glial cells appeared to attach better to polypyrrole/SLPF-coated electrodes than to uncoated gold electrodes. Neuroblastoma cells grew preferentially on and around the polypyrrole/CDPGYIGSR-coated electrode sites while the polypyrrole/CH(3)COO(-)-coated sites on the same probe did not show a preferential attraction to the cells. These results indicate that we can adjust the chemical composition, morphology, electronic transport, and bioactivity of polymer coatings on electrode surfaces on a multichannel micromachined neural probe by controlling electrochemical deposition conditions.

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“…Such solutions and dispersions can then be used to create patterned 2-dimensional surfaces using traditional methods such as ink-jet printing, or to create 3-dimensional (3D) structures using electrospinning techniques. The development of protocols that result in ordered structures at the nanodomain will also impact on the biomaterials field (Boeckl et al 1998), nanotopography having already been shown to have a dramatic effect on fibroblast response (Dalby et al 2002). Strategies to enable synthesis of nanocomponents of controlled dimensions, as well as the assembly of nanodimensional structures, are being developed at a rapid rate (Innis and Wallace 2002).…”

Section: Discussionmentioning

confidence: 99%

Bionic Ears: Their Development and Future Advances Using Neurotrophins and Inherently Conducting Polymers

Clark

Wallace

2004

Applied Bionics and Biomechanics

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Abstract:The development of the multiple-channel bionic ear for hearing and speech understanding in profoundly deaf people is the result of integrating biological and physical sciences with engineering. It is the first clinically successful restoration of sensory and brain function, and brings electronic technology into a direct functional relationship with human consciousness. It presently transmits essential place and coarse temporal information for the coding of frequency, but the fine temporal and place excitation of groups of nerve fibres is inadequate for high-fidelity sound. This is required for adequate musical appreciation and hearing in noise. Research has demonstrated that nerve growth factors preserve the peripheral processes of the auditory nerves so that an electrode array placed close to these fibres could produce this fine temporal and spatial coding. The nerve growth factors can be incorporated into inherently conducting polymers that are part of the array so the peripheral processes can be preserved at the same time as they are electrically stimulated.

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Template-assisted nano-patterning of solid surfaces

Cited by 19 publications

References 14 publications

Characterization of Adsorbed Protein Films by Time-of-Flight Secondary Ion Mass Spectrometry with Principal Component Analysis

Characterization of Adsorbed Protein Films by Time-of-Flight Secondary Ion Mass Spectrometry with Principal Component Analysis

Surface modification of neural recording electrodes with conducting polymer/biomolecule blends

Bionic Ears: Their Development and Future Advances Using Neurotrophins and Inherently Conducting Polymers

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