Temperature-tuned DNA condensation and gene transfection by PEI-g-(PMEO2MA-b-PHEMA) copolymer-based nonviral vectors

Yang, Jianhai; Zhang, Peng; Tang, Lei; Sun, Peng; Liu, Wenguang; Sun, Pei; Zuo, Aijun; Liang, Dongchun

doi:10.1016/j.biomaterials.2009.09.027

Cited by 64 publications

(43 citation statements)

References 32 publications

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“…In gene delivery studies where thermoresponsive polymers were used the temperature at which one or two of the aforementioned steps were performed at was changed. In particular, in studies where polyethyleneimmine (PEI) with grafted PNIPAAm [43], chitosan grafted with PNIPAAm [44], linear and branched NIPAAm, DMAEMA and PEI polymers [2] and PEG polymers with grafted PEI chains [45] were used, the complexation and transfection temperature were changed to enhance the transfection efficiency. In other studies only the incubation or complexation temperature were varied using random terpolymers of P(NIPAAm-co-DMAEMA-co-BuMA) [18,46] or PNIPAAm copolymers [47], while both complexation and incubation temperature were varied using a polyarginine polymer conjugated with PNIPAAm [48].…”

Section: Gene Deliverymentioning

confidence: 99%

Thermoresponsive Polymers for Biomedical Applications

2011

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Thermoresponsive polymers are a class of "smart" materials that have the ability to respond to a change in temperature; a property that makes them useful materials in a wide range of applications and consequently attracts much scientific interest. This review focuses mainly on the studies published over the last 10 years on the synthesis and use of thermoresponsive polymers for biomedical applications including drug delivery, tissue engineering and gene delivery. A summary of the main applications is given following the different studies on thermoresponsive polymers which are categorized based on their 3-dimensional structure; hydrogels, interpenetrating networks, micelles, crosslinked micelles, polymersomes, films and particles.

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Section: Gene Deliverymentioning

confidence: 99%

Thermoresponsive Polymers for Biomedical Applications

2011

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“…When the mass ratio of mPEG-b-P(MMD-co-LA) to P(MMD-co-LA)-g-PEI was 3:1, the increasing size of MPs/pEGFP-ZNF580 at N/P molar ratio of 5 was mainly due to the introduction of plasmid, while the reducing trend of size at higher N/P ratios may result from better plasmidcondensing effect and more compact structure of the complexes compared with that at N/P =5. 40 The complexes with other mass ratios (2:2 and 1:3) of mPEG-b-P(MMD-co-LA) to P(MMD-co-LA)-g-PEI also showed the same tendency. More importantly, all of the MPs and the complexes were less than 120 nm and beneficial for endocytosis.…”

Section: Abbreviationsmentioning

confidence: 78%

Co-self-assembly of cationic microparticles to deliver pEGFP-ZNF580 for promoting the transfection and migration of endothelial cells

Feng¹,

Guo²,

Li³

et al. 2016

IJN

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“…In addition, the bromine in the HMB could initiate the ATRP, this new thiolation route could also be extended to produce thiol end-functioned ATRP polymer. The biocompatible polymer PMEO 2 MA, which had been used in a wide spectrum of biological applications 37-39 was produced through this strategy (Supplementary Material: Scheme S1) and selected as hydrophilic brush.…”

Section: Resultsmentioning

confidence: 99%

Theranostic Self-Assembly Structure of Gold Nanoparticles for NIR Photothermal Therapy and X-Ray Computed Tomography Imaging

et al. 2014

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The controllable self-assembly of amphiphilic mixed polymers grafted gold nanoparitcles (AuNPs) leads to strong interparticle plasmonic coupling, which can be tuned to the near-infrared (NIR) region for enhanced photothermal therapy (PTT). In this study, an improved thiolation method was adopted for ATRP and ROP polymer to obtain amphiphilic brushes of PMEO2MA-SH and PCL-SH. By anchoring PCL-SH and PMEO2MA-SH onto the 14 nm AuNPs, a smart hybrid building block for self-assembly was obtained. Increasing the PCL/PMEO2MA chain ratio from 0.8:1, 2:1 and 3:1 to 7:1, the structure of gold assemblies (GAs) was observed to transfer from vesicle to large compound micelle (LCM). Contributed to the special dense packed structure of gold nanoparticles in LCM, the absorption spectrometry of gold nanoparticles drastically red-shifted from 520 nm to 830 nm, which endowed the GAs remarkable NIR photothermal conversion ability. In addition, gold has high X-ray absorption coefficient which qualifies gold nanomaterial a potential CT contrast agent Herein, we obtain a novel gold assembly structure which can be utilized as potential photothermal therapeutic and CT contrast agents. In vitro and In vivo studies testified the excellent treatment efficacy of optimum GAs as a PTT and CT contrast agent. In vitro degradation test, MTT assay and histology study indicated that GAs was a safe, low toxic reagent with good biodegradability. Therefore, the optimum GAs with strong NIR absorption and high X-ray absorption coefficient could be used as a theranostic agent and the formation of novel gold large compound micelle might offers a new theory foundation for engineering design and synthesis of polymer grafted AuNPs for biomedical applications.

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Temperature-tuned DNA condensation and gene transfection by PEI-g-(PMEO2MA-b-PHEMA) copolymer-based nonviral vectors

Cited by 64 publications

References 32 publications

Thermoresponsive Polymers for Biomedical Applications

Thermoresponsive Polymers for Biomedical Applications

Co-self-assembly of cationic microparticles to deliver pEGFP-ZNF580 for promoting the transfection and migration of endothelial cells

Theranostic Self-Assembly Structure of Gold Nanoparticles for NIR Photothermal Therapy and X-Ray Computed Tomography Imaging

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