Temperature-Tolerated Mainstream Nitrogen Removal by Anammox and Nitrite/Nitrate-Dependent Anaerobic Methane Oxidation in a Membrane Biofilm Reactor

Liu, Tao; Lim, Zhuan Khai; Chen, Hui; Hu, Shihu; Yuan, Zhiguo; Guo, Jianhua

doi:10.1021/acs.est.9b05650

Cited by 60 publications

(20 citation statements)

References 44 publications

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“…Numerous studies have elucidated the effects of operational factors (e.g., temperature and dissolved oxygen (DO)) on anammox performance and the microbial community based on 16S rRNA gene or metagenomic analysis [ 19 – 22 ]. Although the microbial composition and metabolic potential can be demonstrated by metagenomic data, the changes in metabolic activities revealed by metatranscriptomic data are also of critical importance to explore how microbes respond to short-term disturbance when the community structure does not change significantly [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Response and resilience of anammox consortia to nutrient starvation

Wang

Liu

et al. 2022

Microbiome

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Background It is of critical importance to understand how anammox consortia respond to disturbance events and fluctuations in the wastewater treatment reactors. Although the responses of anammox consortia to operational parameters (e.g., temperature, dissolved oxygen, nutrient concentrations) have frequently been reported in previous studies, less is known about their responses and resilience when they suffer from nutrient interruption. Results Here, we investigated the anammox community states and transcriptional patterns before and after a short-term nutrient starvation (3 days) to determine how anammox consortia respond to and recover from such stress. The results demonstrated that the remarkable changes in transcriptional patterns, rather than the community compositions were associated with the nutritional stress. The divergent expression of genes involved in anammox reactions, especially the hydrazine synthase complex (HZS), and nutrient transportation might function as part of a starvation response mechanism in anammox bacteria. In addition, effective energy conservation and substrate supply strategies (ATP accumulation, upregulated amino acid biosynthesis, and enhanced protein degradation) and synergistic interactions between anammox bacteria and heterotrophs might benefit their survival during starvation and the ensuing recovery of the anammox process. Compared with abundant heterotrophs in the anammox system, the overall transcription pattern of the core autotrophic producers (i.e., anammox bacteria) was highly resilient and quickly returned to its pre-starvation state, further contributing to the prompt recovery when the feeding was resumed. Conclusions These findings provide important insights into nutritional stress-induced changes in transcriptional activities in the anammox consortia and would be beneficial for the understanding of the capacity of anammox consortia in response to stress and process stability in the engineered ecosystems.

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Section: Introductionmentioning

confidence: 99%

Response and resilience of anammox consortia to nutrient starvation

Wang

Liu

et al. 2022

Microbiome

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“…An effluent with 3 mg TN/L was achieved simultaneously by integrating anammox and DAMO reactions in mainstream wastewater treatment (Xie et al, 2018). A stable NRR of 0.13 kg/m 3 /d, together with a high-level effluent quality (TN < 5.0 mg /L) was also achieved in a laboratory-scale upflow MBfR by the DAMO/A process at a temperature as low as 10°C (Liu et al, 2020). The third process, named the onestage PN-DAMO/A, integrates PN, anammox and DAMO in a single MBfR, as shown in Fig.…”

Section: Damo/a Processmentioning

confidence: 99%

“…Based on the core mechanism and actual feasibility, three comprehensive and practical anammox-based processes have garnered most of the research attention: the PN/A process (Chen et al, 2019), the PD/A process (Ma et al, 2017;Zhou et al, 2020), and the DAMO/A process (Shi et al, 2013;Liu et al, 2020). The nitrogen removal pathway of these processes are shown in Fig.…”

Section: The Main Anammox-based Processesmentioning

confidence: 99%

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The main anammox-based processes, the involved microbes and the novel process concept from the application perspective

Guo

Luo

Shen

et al. 2021

Front. Environ. Sci. Eng.

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Anammox technology has been widely researched over the past 40-year from the laboratory-scale to full-scale. It is well-known that in actual applications, the solo application of anammox is not feasible. Since both ammonium and nitrite are prerequisites based on the reaction mechanism, the pre-treatment of wastewater is necessary. With the combination of anammox process and other pre-treatment processes to treat the actual wastewater, many types of anammox-based processes have been developed with distinct nitrogen removal performance. Thus, in order to heighten the awareness of researchers to the developments and accelerate the application of these processes to the treatment of actual wastewater, the main anammox-based processes are reviewed in this paper. It includes the partial nitritation/anammox process, the denitratation/anammox (PD/A) process, the denitrifying anaerobic methane oxidation/anammox (DAMO/A) process, and more complex deuterogenic processes. These processes have made the breakthroughs in the application of the anammox technology, such as the combination of nitrification and PD/A process can achieve stability and reliability of nitrogen removal in the treatment of mainstream wastewater, the PD/A process and the DAMO/A have brought about further improvements in the total nitrogen removal efficiency of wastewater. The diversity of functional microbe characteristics under the specific condition indicate the wide application potential of anammox-based processes, and further exploration is necessary. A whole waste treatment system concept is proposed through the effective allocation of above mentioned processes, with the maximum recovery of energy and resources, and minimal environmental impact.

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“…Anaerobic ammonium oxidation (anammox) as an autotrophic nitrogen removal process has been developed to remove nitrogen from the wastewater with low COD/TN ratio because of its no requirement for organic carbon, reducing oxygen consumption, and excess sludge production (Liu et al, 2020; Liu, Khai Lim, et al, 2020). However, the application of anammox process is often limited by the slow growth rate of anammox bacteria (Liu & Ni, 2015).…”

Section: Introductionmentioning

confidence: 99%

Nitrogen removal from domestic wastewater in a novel hybrid anoxic‐oxic biofilm reactor at different reflux ratios

Burov

Meng

2020

Water Environment Research

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A lab‐scale hybrid anoxic‐oxic biofilm reactor (HAOBR) with one anoxic compartment and two oxic compartments in sequence was constructed to treat domestic wastewater in this study. Performance of the HAOBR was evaluated at 25°C and hydraulic retention time 12 hr with reflux ratio increased from 100% to 300% by stages. The results showed that COD, NH4+‐N, and TN removal presented an increasing trend with the increase of reflux ratio by stages. At the optimal reflux ratio of 300%, removal of COD, NH4+‐N, and TN averaged 83.9%, 99.0%, and 80.8%, respectively. Analysis about pollutant concentration in each compartment of the HAOBR revealed that the excellent pollutant removal was mainly achieved by the cooperation of nitrification in 3rd oxic compartment and denitrification in 1st anoxic compartment. Denitrification in the anoxic zone of 2nd oxic compartment would also contribute to the nitrogen removal. The higher nitrogen removal of the HAOBR at the reflux ratio of 300% is attributed to the presence of the anammox in the 1st anoxic compartment, which is mainly due to the lower COD concentration in the compartment at the higher reflux ratio. Practitioner points A hybrid anoxic‐oxic baffled reactor was built to treat domestic wastewater. Effect of reflux ratio and mechanism of nitrogen removal were investigated. Reflux ratio 300% was favorable for COD, NH4+ and TN removal. The removal of COD, NH4+ and TN averaged 84.4%, 99.0% and 80.8%, respectively. Cooperation of nitrification, denitrification and anammox dominated the high nitrogen removal at the higher reflux ratio of 300%.

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Temperature-Tolerated Mainstream Nitrogen Removal by Anammox and Nitrite/Nitrate-Dependent Anaerobic Methane Oxidation in a Membrane Biofilm Reactor

Cited by 60 publications

References 44 publications

Response and resilience of anammox consortia to nutrient starvation

Response and resilience of anammox consortia to nutrient starvation

The main anammox-based processes, the involved microbes and the novel process concept from the application perspective

Nitrogen removal from domestic wastewater in a novel hybrid anoxic‐oxic biofilm reactor at different reflux ratios

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