Temperature shifts induce intraspecific variation in microalgal production and biochemical composition

Sayegh, Fotoon; Montagnes, David J. S.

doi:10.1016/j.biortech.2010.10.011

Cited by 36 publications

(13 citation statements)

References 30 publications

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“…At 25℃, this microalgae exhibited a very low and almost no grow in terms of cell density, this temperature led to an abrupt interruption of microalgal growth and later the cell dead on further period of cultivation. Previous studies by Sayegh and Montagnes (2010); James et al (1989) and Brown and Jeffrey (1992) found that Nannochloropsis sp. grown optimally at temperature around 20℃.…”

Section: Discussionmentioning

confidence: 90%

Effect of Temperature and Nutrient Limitation on the Growth and Lipid Content of Three Selected Microalgae (Dunaliella tertiolecta, Nannochloropsis sp. and Scenedesmus sp.) for Biodiesel Production

Rukminasari¹

2013

ijms

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Microalgae is one of potential source for biodiesel due to high efficiency of solar energy conversion to chemical energy.Several microalgae also have high lipid content per dry weight of biomass. The aims of the present work to study the effects of temperature and nutrient depletion on the growth and lipid content of three selected microalgae (Dunaliella tertiolecta, Nannochloropsis sp and Scenedesmus sp) in view of their possible utilization as raw materials for biodiesel production. In addition, various lipid analysis methods were applied, such as gravimetric, Nile Red staining and FTIR spectroscopy. Algal growth and lipid content was strongly influenced by the variation of tested parameters; indeed, an increase or decrease temperature from ambient temperature and nutrient depletion practically increase in lipid content. Nile Red staining and FTIR spectroscopy are effective tool to analyze rapidly of lipid content from selected microalgae.

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Section: Discussionmentioning

confidence: 90%

Effect of Temperature and Nutrient Limitation on the Growth and Lipid Content of Three Selected Microalgae (Dunaliella tertiolecta, Nannochloropsis sp. and Scenedesmus sp.) for Biodiesel Production

Rukminasari¹

2013

ijms

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“…This is because of photooxidation reaction in the cells, owed to excess light that cannot be absorbed by the photosynthetic apparatus. The changes in pigments are related to an adaptation mechanism, chlorophyll was reduced due to photooxidation, and carotenoids were increased to protect photooxidative damage of the cell [19], in addition to that, in higher light the algae synthesized smaller photosynthetic units, most probably to prevent photo damage, however, in low light larger photosynthetic units are found probably to aid light harvesting [22].In general, rapidly growing microalgal cells were exhibited by a higher protein and low carbohydrate content [4], so the rapidly growing cells of natural day light at 25-30°C showed higher amount of protein. Similar observations found in Ankistrodesmus fusiformis [23].…”

mentioning

confidence: 99%

“…These factors influence the nutritional value of phytoplankton such as protein, carbohydrate, lipid, amino acid and pigments composition [3]. These specific chemicals attributes will not follow similar trends with changing temperature and light conditions [4]. The effects of irradiance and photoperiod on the biomass and fatty acid composition of Chlorella vulgaris, were also examined [5].…”

Section: Introductionmentioning

confidence: 99%

Effects of Culture Conditions on Growth and Biochemical Profile of Chlorella Vulgaris

Sharma¹,

Singh²,

Sharma³

2012

J Plant Pathol Microb

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The effects of culture conditions at different temperature and light regimes on growth and the contents of chlorophyll-a, chlorophyll-b, total carotenoids, total protein and total free amino acids of Chlorella vulgaris were determined. The growth of C. vulgaris in terms of optical density (0.42 at 670 nm), cell count (440 x 104 cells/ml) and dry weight (30.2 mg/50 ml), and the amount of chlorophyll-a (2.16%), chlorophyll-b (0.59%) and total protein, was found higher at the temperature 25-30°C and natural day light receiving through the north facing window of the growth room. Although, the amount of total carotenoids (0.440%) and free amino acids (834 µg/gm fresh weight) were found maximum in continuous light at 30-35°C, not much differences in the amount of carotenoids (0.385%) and free amino acids (822 µg/gm fresh weight) were found at 25-30°C and natural day light. The natural day light at 25-30°C was also proved proficient, as distinct banding pattern with unique polypeptides such as 15KDa, 47KDa and 50KDa, on the other hand, 23KDa, 26KDa and 36KDa appeared in all samples, these bands were not affected by light and temperature. Our results indicate that among all five culture conditions tested, the cultures kept at north facing window receiving natural day light at temperature 25-30°C, show best growth and higher contents of biochemicals that will be beneficial to use Chlorella for high nutritive purpose. In order to find out the optimum culture condition, the cultures were subjected to five different conditions of temperature and light regimes. In the growth room, light was provided by fluorescent tube lights (40 W) having 2500 Lux intensity, and were fixed at a distance of 64 cm from the cultures in constant and alternate light conditions. One set of five different culture conditions were placed in the laboratory in window, which was facing north, for providing natural day and dark periods. The intensity of natural day light was as an average 2700 Lux. The following culture conditions set in the growth room were used in the present study: Effects of Culture Conditions on Growth and Biochemical Profile of Chlorella Vulgaris Rekha Growth measurement Estimation of pigmentsChlorophyll content of the samples were extracted in 90% (v/v) acetone, and chlorophyll-a, b estimated by Parson and Strickland method [14]. Carotenoid content of the samples were extracted in 80% (v/v) acetone, and estimated by Jensen method [15]. Estimation of proteinsProtein content of the samples was estimated quantitatively by Lowry [16] by dry biomass, using bovine serum albumin (BSA) as standard. Qualitatively, the proteins were estimated by the SDS-PAGE analysis, which was carried out according to Laemmli [17]. For extraction of proteins, the samples were homogenized with lysis buffer containing 0.5M Tris-HCl, 8M Urea, 5% (w/v) SDS, 20% (v/v) Glycerol and 10% (v/v) β-Mercaptoethanol; final pH 6.8 and centrifuged at 4°C for 20 min at 10,000 rpm. Protein extract was used for SDS-PAGE analysis, using a 12% polyacrylamide gel co...

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“…It is generally recognized that microalgal cell sizes are inversely proportional to their growth rate (Sayegh and Montagnes, 2011;Chen et al, 2012). However in our studies, according to flow cytometer results, the cell size of the grown algae were larger at 70% and 100% AAS effluent loadings with higher growth rates.…”

Section: Aerated Culturesmentioning

confidence: 99%

Cultivating and harvesting of marine alga Nannochloropsis oculata in local municipal wastewater for biodiesel

Şirin

Sillanpää

2015

Bioresource Technology

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Temperature shifts induce intraspecific variation in microalgal production and biochemical composition

Cited by 36 publications

References 30 publications

Effect of Temperature and Nutrient Limitation on the Growth and Lipid Content of Three Selected Microalgae (<i>Dunaliella tertiolecta</i>, <i>Nannochloropsis</i> sp. and <i>Scenedesmus</i> sp.) for Biodiesel Production

Effect of Temperature and Nutrient Limitation on the Growth and Lipid Content of Three Selected Microalgae (<i>Dunaliella tertiolecta</i>, <i>Nannochloropsis</i> sp. and <i>Scenedesmus</i> sp.) for Biodiesel Production

Effects of Culture Conditions on Growth and Biochemical Profile of Chlorella Vulgaris

Cultivating and harvesting of marine alga Nannochloropsis oculata in local municipal wastewater for biodiesel

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