Temperature sensitive radiosensitive mutants of the yeast Saccharomyces paradoxus

Bandas, E.L.; Bekker, M L; Luchkina, L A; Tkatchenko, V. P.; Захаров, И. А.

doi:10.1007/bf00330990

Cited by 4 publications

(1 citation statement)

References 9 publications

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“…Conditional lethal mutants, in which growth and other metabolic features can be controlled simply by altering conditions such as temperature, have proved to be very powerful tools in genetic and biochemical studies (1,6,8,15). In Schizosaccharomyces pombe, a large number of temperature-sensitive mutants have been isolated and characterized (3).…”

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Temperature-sensitive mutant of Schizosaccharomyces pombe exhibiting enhanced radiation sensitivity

Duck¹,

Nasim²,

James³

1976

J Bacteriol

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A conditional lethal and radiation-sensitive mutant of Schizosaccharomyces pombe is described in which both characteristics result from a single gene mutation. Confirmation of the pleiotropic nature of this mutant was obtained by tetrad analysis and by testing the radiation sensitivity of a large number of revertants that grew normally at the restrictive temperature. The colony-forming ability of the mutant after ultraviolet radiation, gamma radiation, and ethyl methane sulfonate treatment is considerably altered by the post-treatment incubation temperature, showing higher survival at 25 than at 30degreesC. The radiosensitivity of the mutant is also influenced by the stage of growth. The difference in radiation sensitivity between the wild type and mutant is greater when log-phase cultures are compared. The characteristics of this mutant suggest that it is defective in a step common to both deoxyribonucleic acid replication and repair.

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confidence: 99%

Temperature-sensitive mutant of Schizosaccharomyces pombe exhibiting enhanced radiation sensitivity

Duck¹,

Nasim²,

James³

1976

J Bacteriol

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Isolation and characterization of yeast mutants with thermoconditional sensitivity to the bifunctional alkylating agent nitrogen mustard

Siede

Brendel

1981

Current Genetics

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Selection of mutants of Saccharomyces cerevisiae sensitive to the DNA cross-linking agent nitrogen mustard (HN2) at two temperatures (23 °C and 36 °C) yielded two isolates with thermoconditionally enhanced (ts) sensitivity to the mutagen. Both were due to single recessive nuclear genes. Mutant allele snm1-2 (ts) showed mainly ts-sensitivity to HN2, whereas mutant allele snm2-1 (ts) conferred ts-sensitivity to HN2, half mustard (HN1) and UV. In temperature-shift experiments it was determined that the functions of SNM1 and SNM2 are needed for recovery within 6 to 7 h. after mutagen exposure during incubation at 23 °C on YEPD when HN2 and UV are applied. After HN1 treatment the SNM2 coded function is required for recovery for about 14 hrs. This possibly indicates a handling of UV- and HN2-induced lesions different from that of HN1-induced lesions.

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Chapter 12 Induction, Selection, and Experimental Uses of Temperature-Sensitive and Other Conditional Mutants of Yeast

Pringle

1975

Methods in Cell Biology

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Temperature sensitive radiosensitive mutants of the yeast Saccharomyces paradoxus

Cited by 4 publications

References 9 publications

Temperature-sensitive mutant of Schizosaccharomyces pombe exhibiting enhanced radiation sensitivity

Temperature-sensitive mutant of Schizosaccharomyces pombe exhibiting enhanced radiation sensitivity

Isolation and characterization of yeast mutants with thermoconditional sensitivity to the bifunctional alkylating agent nitrogen mustard

Chapter 12 Induction, Selection, and Experimental Uses of Temperature-Sensitive and Other Conditional Mutants of Yeast

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