Temperature-Sensitive Mutations in Drosophila melanogaster

Suzuki, David T

doi:10.1007/978-1-4615-7145-2_23

Cited by 15 publications

(20 citation statements)

References 82 publications

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“…The data are shown in Fig. 3 which signals the end of the critical period (Suzuki, 1970). Taken together, these data define the existence of a critical period in larval development which begins no later than 60 h after oviposition and which ends no earlier than 120 h after oviposition, during which the developing larva must be exposed to HD conditions if an ELP is to be expressed.…”

Section: Statistical Analysis Of Longevitymentioning

confidence: 93%

Larval regulation of adult longevity in a genetically-selected long-lived strain of Drosophila

et al. 1993

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Our previous work has shown that the major genes involved in the expression of the extendedlongevity phenotype are located on the third chromosome. Furthermore, their expression is negatively and positively influenced by chromosomes 2 and 1, respectively. In this report we show that the expression of the extended-longevity phenotype is dependent on the larval environment. A controlled chromosome substitution experiment was carried out using a strain selected for long life (L) and its parent (R) strain. Twenty different combinations of the three major chromosomes were conducted and their longevities were determined under both high (HD) and low (LD) larval density conditions. The extended-longevity phenotype was only expressed under RD conditions. The chromosome interactions were not apparent under LD conditions. Density-shift experiments delineate a critical period for expression of the extended-longevity phenotype, extending from 60 h after egg laying (AEL) to 96 h AEL, during which the developing animal must be exposed to HD conditions if the extended-longevity phenotype is to be expressed. The change from HD to LD conditions is accompanied by statistically significant increases in body weight. The possible role of a dietary restriction phenomenon is examined and the implications of these findings discussed. It is now apparent, however, that the extended-longevity phenotype in Drosophila is a developmental genetic process.

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Section: Statistical Analysis Of Longevitymentioning

confidence: 93%

Larval regulation of adult longevity in a genetically-selected long-lived strain of Drosophila

et al. 1993

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“…With the aim of saturation, approximately five times as many test lines had to be established and screened. To avoid tedious sorting of flies, we eliminated unwanted progeny by using dominant temperature-sensitive mutations (Suzuki 1970) and by growing the adults to be used in the test generation at high temperature. After two generations of inbreeding, females and males heterozygous for the mutagenized chromosome emerged (Figure 5).…”

Section: Mutagenesis and Scoringmentioning

confidence: 99%

The Heidelberg Screen for Pattern Mutants of Drosophila: A Personal Account

Wieschaus

Nüsslein‐Volhard

2016

Annu. Rev. Cell Dev. Biol.

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In large-scale mutagenesis screens performed in 1979-1980 at the EMBL in Heidelberg, we isolated mutations affecting the pattern or structure of the larval cuticle in Drosophila. The 600 mutants we characterized could be assigned to 120 genes and represent the majority of such genes in the genome. These mutants subsequently provided a rich resource for understanding many fundamental developmental processes, such as the transcriptional hierarchies controlling segmentation, the establishment of cell states by signaling pathways, and the differentiation of epithelial cells. Most of the Heidelberg genes are now molecularly known, and many of them are conserved in other animals, including humans. Although the screens were initially driven entirely by curiosity, the mutants now serve as models for many human diseases. In this review, we describe the rationale of the screening procedures and provide a classification of the genes on the basis of their initial phenotypes and the subsequent molecular analyses.

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“…Environmentally conditioned mutations covering the whole genome can easily be obtained; in particular, the fruitfly (Drosophila melanogaster) has been used extensively as a model organism in this context (Suzuki, 1970;Tasaka and Suzuki, 1973;Arking, 1975;Mitchell and Petersen, 1982;Homyk et al, 1986). Detailed genetic and molecular studies of relatively simple conditionally expressed genetic defects can contribute to an improved understanding of the causations of inbreeding depression, and identify the cascades of processes affected by expression of a single or few deleterious mutations with major fitness effects.…”

Section: Introductionmentioning

confidence: 99%

Proteomic characterization of a temperature-sensitive conditional lethal in Drosophila melanogaster

et al. 2009

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Genetic variation that is expressed only under specific environmental conditions can contribute to additional adverse effects of inbreeding if environmental conditions change. We present a proteomic characterization of a conditional lethal found in an inbred line of Drosophila melanogaster. The lethal effect is apparent as a large increase in early mortality at the restrictive temperature (29 1C) as opposed to normal survival at the permissive temperature (20 1C). The increased mortality in response to the restrictive temperature is probably caused by a single recessive major locus. A quantitative trait locus (QTL) region segregating variation affecting the lethal effect has been identified, allowing for a separation of primary/causal effects and secondary consequences in the proteome expression patterns observed. In this study, the proteomic response to the restrictive temperature in the lethal-line (L-line) was compared with the response in an inbred-control-line (IC-line) and an outbred-control-line (OC-line). Quantitative protein changes were detected using isobaric tags for relative and absolute quantitation (iTRAQ) two-dimensional liquid chromatography-tandem mass spectrometry. In all, 45 proteins were found to be significantly differently regulated in response to the restrictive temperature in the L-line as compared with the IC-line. No proteins were significantly differently regulated between the IC-line and the OC-line, verifying that differential protein regulation was specific to a genetic defect in the L-line. Proteins associated with oxidative phosphorylation and mitochondria were significantly overrepresented within the list of differentially expressed proteins. Proteins related to muscle contraction were also found to be differentially expressed in the L-line in response to the restrictive temperature, supporting phenotypic observations of moribund muscle hypercontraction.

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Temperature-Sensitive Mutations in Drosophila melanogaster

Cited by 15 publications

References 82 publications

Larval regulation of adult longevity in a genetically-selected long-lived strain of Drosophila

Larval regulation of adult longevity in a genetically-selected long-lived strain of Drosophila

The Heidelberg Screen for Pattern Mutants of Drosophila: A Personal Account

Proteomic characterization of a temperature-sensitive conditional lethal in Drosophila melanogaster

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