Temperature-sensitive microcapsules with variable optical signatures based on incorporation of quantum dots into a highly biocompatible hydrogel

Hardison, Daniel; Halambage, Upul D.; Senevirathna, Wasana; Pathirathne, Thusitha; Wells, Mona

doi:10.1039/b811905h

Cited by 18 publications

(23 citation statements)

References 70 publications

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“…Recently, molecular-level thermometry has been demonstrated using fluorescent nanohydrogels (8,9). While nanogels are effective for observing temperature changes at an intracellular level, their effective range and sensitivity are limited, and mid-to long-term biocompatibility studies have yet to be conducted (8,9). We report here an alternative approach to intracellular thermometry, with a 20°C range of detection and at least 0.7°C sensitivity between 35 and 45°C.…”

Section: Discussionmentioning

confidence: 99%

“…As contextual challenges to this practice evolve (7) and therapies relying on accurate application of local heating have developed (11) need for molecular-level thermometry in biological systems is an emerging and important frontier (15). Recently, molecular-level thermometry has been demonstrated using fluorescent nanohydrogels (8,9). While nanogels are effective for observing temperature changes at an intracellular level, their effective range and sensitivity are limited, and mid-to long-term biocompatibility studies have yet to be conducted (8,9).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

LacI(Ts)-Regulated Expression as an In Situ Intracellular Biomolecular Thermometer

McCabe

Lacherndo

Albino-Flores

et al. 2011

Appl Environ Microbiol

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In response to needs for in situ thermometry, a temperature-sensitive vector was adapted to report changes in the intracellular heat content of Escherichia coli in near-real time. This model system utilized vectors expressing increasing quantities of ␤-galactosidase in response to stepwise temperature increases through a biologically relevant range (22 to 45°C). As judged by calibrated fluorometric and colorimetric reporters, both whole E. coli cells and lysates expressed significant repeatable changes in ␤-galactosidase activity that were sensitive to temperature changes of less than 1°C (35 to 45°C). This model system suggests that changes in cellular heat content can be detected independently of the medium in which cells are maintained, a feature of particular importance where the medium is heterogeneous or nonaqueous, or otherwise has a low heat transfer capacity. We report here that the intracellular temperature can be reliably obtained in near-real time using reliable fluorescent reporting systems from cellular scales, with a 20°C range of detection and at least 0.7°C sensitivity between 35 and 45°C.Temperature is a master environmental variable, whose accurate measurement is critical for understanding biological processes from the molecular to the biome level (13,15). At a molecular level, temperature in biological systems is typically measured by observing the surrounding growth medium, and while the rapid thermal relaxation of the cellular milieu and aqueous medium makes this approximation valid under many circumstances, there are notable exceptions. Rapid cellular and subcellular heat content changes have been theorized to result from alternating electromagnetic fields (EMF) (7), and the continued development of hyperthermia-based therapies combining nanomaterials and EMF (11) requires the development of in situ and in vivo temperature measurement for subcellular and tissue levels.Cellular heat content affects biomolecular assembly, structure, and function. It has long been recognized that cellular systems sense temperature through various means, including transcription factor binding and mRNA secondary structure (3,5,13,15,16,21). In vivo, these systems maintain homeostasis by responding to environmental temperature changes (5,6,16,(20)(21)(22). Subtle changes in biomolecular structure alter the temperature response, as evidenced by temperature-sensitive mutations (2). While these systems have been engineered or are otherwise exploited for functional studies and expression control, none of these heat-sensing systems have been developed for intracellular thermometry (15). We report here the first demonstration of a biomolecular intracellular thermometer. This system utilizes temperature-sensitive mutants of lacI controlling LacZ expression (3, 4), which accurately reports cellular temperature in an environmentally relevant range.Constitutive expression of LacI(Ts) by a T7 promoter (derived from pET24; Novagen/EMD, Darmstadt, Germany) results in the availability of LacI(Ts) to bind lacO sites. At permis...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

LacI(Ts)-Regulated Expression as an In Situ Intracellular Biomolecular Thermometer

McCabe

Lacherndo

Albino-Flores

et al. 2011

Appl Environ Microbiol

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“…[3][4][5] Depending upon the functionalities of the NPs, these capsules have applications in diverse fi elds such as in drug delivery, optical biosensing, catalysis, among others. [6][7][8][9][10] At present, a diversity of methods has been reported for the fabrication of capsules using either soft or hard templates. These include poly(styrene) beads, [ 11 ] SiO 2 colloids, [ 12 ] vesicles Part.…”

Section: Introductionmentioning

confidence: 99%

Multiple Emulsion Templating of Hybrid Ag/SiO₂ Capsules for Antibacterial Applications

Sousa

Almeida

Girão

et al. 2014

Part & Part Syst Charact

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Silver nanoparticles (NPs) encapsulated in amorphous silica shells are synthesized and evaluated for their antibacterial action using the Gram‐negative Escherichia coli bacterium. These inorganic capsules are synthesized using a new approach that comprises the use of oil‐in‐water‐in‐oil (O/W/O) multiple emulsions to fabricate SiO2 capsules incorporating organically capped Ag NPs. This strategy is explored as a mean to promote the bioadhesion of the microorganisms to the silica rough surfaces while still keeping the system with a high surface area for the active metal. The results have shown that the hybrid capsules enable a slow release of cationic silver from the interior of the silica microsphere to the external medium probably through the pore channels in the shell. The antibacterial activity against E. coli is mainly determined by the Ag+ ion release rate, suggesting that these particulates can be employed as a robust system for prolonged used as an antimicrobial material.

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“…Moreover, Wells et al prepared the temperature responsive microcapsules with variable optical signatures based on the incorporation of quantum dots into a highly biocompatible hydrogel [66]. The resulting microcapsules displayed temperature responsive behavior, as the optical signature displayed a linear dependence on temperature over the range of 20-45°C.…”

Section: Application Of Temperature Responsive Hydrogelsmentioning

confidence: 99%

“…These nanoparticles were stabilized in the hydrogel network and the chlorination reactivity in water was enhanced and controllable in the temperature range of 30-34°C. Additionally, the methylcellulose based thermally reversible hydrogel system for tissue engineering applications was developed by Devireddy and coworkers [65].Moreover, Wells et al prepared the temperature responsive microcapsules with variable optical signatures based on the incorporation of quantum dots into a highly biocompatible hydrogel [66]. The resulting microcapsules displayed temperature responsive behavior, as the optical signature displayed a linear dependence on temperature over the range of 20-45°C.…”

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confidence: 99%

Temperature Responsive Hydrogels:Construction and Applications

Yin¹

2016

Polym Sci

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IntroductionAs a kind of smart materials, responsive hydrogels exhibit the rapidly responsive ability and sharp volume phase transitions according to the external stimuli from environment. Hydrogels demonstrate large-scale volumetric changes in response to small levels of stimuli. To the best of our knowledge, the most typical external stimuli factors related to responsive behaviors of smart hydrogels are pH [1][2][3][4][5], temperature [6][7][8], light [9][10][11] and multi-factors [12][13][14][15][16]. Additionally, the responsive hydrogels based on enzyme responsive [17,18], calcium responsive [19,20], glucose responsive [21,22], redox responsive [23,24], ionicstrength responsive [25,26], and chemical responsive [27] are also exhibited amazing responsive abilities. Among these insights, temperature responsive hydrogels are particularly important.This article gives an overview of the fundamentals of temperature responsive hydrogels. Some researches related to the construction methods, responsive mechanisms and applications of responsive hydrogels in the past few years are reviewed. Construction Method of Temperature Responsive HydrogelsThe temperature responsive hydrogels introduced in this section are mainly prepared based on the scaffolds of supramolecular assembled structure [28][29][30], hydrogel films [31] and hydrogel sphere [32,33]. Additionally, the temperature responsive hydrogels constructed employing the novel technics are also summarized [34][35][36]. Ritter and coworkers pioneered in construction of temperature responsive hydrogel using N-isopropylacrylamide as monomer [37]. As illustrated in Figure 1 based on the hostguest interaction between adamantyl group and cyclodextrin, the thermosensitive hydrogel 1 was prepared. Similarly, based on the host-guest interaction between D3-symmetric tris(spiroborate) cyclophane and [Ir(tpy) 2 ](PF 6 ) 3 complex, the assembled chain structure was obtained by Yamaguchi et al.[38]. Moreover, employing the host-guest interaction between cucurbit[8]uril and viologen/naphthoxy, a thermo-sensitive supramolecular polymer hydrogel 2 was prepared by Scherman and coworkers [39]. Typically, as the dynamic cross-link of CB[8]/viologen/naphthoxy, this hydrogel exhibited thermal reversibility. Additionally, the temperature responsive hydrogels based on hydrophobic interaction of dipeptides [40] and cyclic macromonomer crosslinking interaction [41] were also reported. Using a photo-crosslinkable benzophenone unit, Knoll and coworkers prepared the responsive hydrogel films by photo-cross-link method based on poly(N-isopropylacrylamide)(PNIPAM) copolymer [42]. To fabricate hydrogel nanoparticles with ultrafine nanosized hair, Yang's group developed a simple self-developed approach AbstractResponsive hydrogels have attracted researcher's special attentions. Up to now, a wide variety of responsive hydrogels are continuously constructed and the responsive mechanisms are deeply investigated. Typically, versatile responsive hydrogels with pH responsive, temperature responsive, ligh...

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Temperature-sensitive microcapsules with variable optical signatures based on incorporation of quantum dots into a highly biocompatible hydrogel

Cited by 18 publications

References 70 publications

LacI(Ts)-Regulated Expression as an In Situ Intracellular Biomolecular Thermometer

LacI(Ts)-Regulated Expression as an In Situ Intracellular Biomolecular Thermometer

Multiple Emulsion Templating of Hybrid Ag/SiO₂ Capsules for Antibacterial Applications

Temperature Responsive Hydrogels:Construction and Applications

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Temperature-sensitive microcapsules with variable optical signatures based on incorporation of quantum dots into a highly biocompatible hydrogel

Cited by 18 publications

References 70 publications

LacI(Ts)-Regulated Expression as an In Situ Intracellular Biomolecular Thermometer

LacI(Ts)-Regulated Expression as an In Situ Intracellular Biomolecular Thermometer

Multiple Emulsion Templating of Hybrid Ag/SiO2 Capsules for Antibacterial Applications

Temperature Responsive Hydrogels:Construction and Applications

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Product

Resources

About

Multiple Emulsion Templating of Hybrid Ag/SiO₂ Capsules for Antibacterial Applications