Temperature responsive functional polymeric thin films obtained by matrix assisted pulsed laser evaporation for cells attachment–detachment study

Rusen, Laurenţiu; Dinca, Valentina; Mitu, B.; Mustăciosu, Cosmin Cătălin; Dinescu, M.

doi:10.1016/j.apsusc.2013.09.122

Cited by 26 publications

(27 citation statements)

References 24 publications

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“…In contrast, below LCST, hydrophilic effect is strengthened and presence of water molecules can consequently increase the swelling behavior of polymer in aqueous solution . Similar to others, we found that wettability properties of synthesized thermoresponsive surfaces shift with temperature changes around LCST [Fig. (B–D)].…”

Section: Discussionsupporting

confidence: 88%

Switchable phase transition behavior of thermoresponsive substrates for cell sheet engineering

Mokhtarinia

Nourbakhsh

Masaeli

et al. 2018

J Polym Sci B Polym Phys

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Recently, there are significant interests in the development of biomaterials with nonlinear response to an external stimulus. Thermoresponsive polymers as a well‐known class of stimuli‐responsive materials represent reversible hydrophilicity/hydrophobicity characteristics around a critical temperature. This switchable behavior applies for nondestructive cellular detachment from cultivation substrates. In this study, poly (N‐isopropylacrylamide) (PNIPAAm)‐grafted dishes were made up to harvest retinal pigmented epithelial (RPE) and periodontal ligament cell (PDLC) sheets. Wettability assessments verified that all functionalized surfaces were inverted from hydrophilic to hydrophobic state when the temperature rises from lower critical solution temperature (LCST) at 37 °C. Other physicochemical characteristics such as chemical composition, grafting thickness, and surface topography were investigated through attenuated total reflection Fourier transform infrared spectroscopy (ATR‐FTIR) and atomic force microscopy (AFM). ATR‐FTIR results showed typical peaks of amide group corresponding to successful PNIPAAm polymerization. AFM microscopy results also proved creating a rough PNIPAAm layer with thickness of 29.2 nm after grafting process in the mixture of methanol and water. Cell culture experiments showed an irreversible cellular attachment/detachment from modified surfaces upon temperature changes. These results introduced thermoresponsive TCPS to noninvasively harvest RPE and PDLCs sheets especially for application in scaffold‐free tissue engineering decorations. © 2018 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2018, 56, 1567–1576

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Section: Discussionsupporting

confidence: 88%

Switchable phase transition behavior of thermoresponsive substrates for cell sheet engineering

Mokhtarinia

Nourbakhsh

Masaeli

et al. 2018

J Polym Sci B Polym Phys

View full text Add to dashboard Cite

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“…The coating films with desired thickness and morphology can be easily tunable through the regulation of experimental parameters and technological variables. Drop-casting is an easier and more accessible method, which usually results in the spreading of polymer solution and the formation of thin film on relatively small substrates after solvent evaporation under programmed conditions [34]. Film thickness is dependent to solution concentration and dispersion volume.…”

Section: Physically Adsorbed Coating Filmsmentioning

confidence: 99%

Preparation and Characterization of Thermoresponsive Poly(N-Isopropylacrylamide) for Cell Culture Applications

et al. 2020

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Poly(N-isopropylacrylamide) (PNIPAAm) is a typical thermoresponsive polymer used widely and studied deeply in smart materials, which is attractive and valuable owing to its reversible and remote "on-off" behavior adjusted by temperature variation. PNIPAAm usually exhibits opposite solubility or wettability across lower critical solution temperature (LCST), and it is readily functionalized making it available in extensive applications. Cell culture is one of the most prospective and representative applications. Active attachment and spontaneous detachment of targeted cells are easily tunable by surface wettability changes and volume phase transitions of PNIPAAm modified substrates with respect to ambient temperature. The thermoresponsive culture platforms and matching thermal-liftoff method can effectively substitute for the traditional cell harvesting ways like enzymatic hydrolysis and mechanical scraping, and will improve the stable and high quality of recovered cells. Therefore, the establishment and detection on PNIPAAm based culture systems are of particular importance. This review covers the important developments and recommendations for future work of the preparation and characterization of temperature-responsive substrates based on PNIPAAm and analogues for cell culture applications.

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“…That is why the choice of surface fabrication methods is directly correlated to the ability to use the smart coating within the desired application. Some of the most used techniques [5,[16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33] for obtaining smart bio-interfaces based on stimuli-responsive polymers within a large area of applications are listed below:…”

Section: Approaches For Obtaining Pnipam Surfacesmentioning

confidence: 99%

“…Among these types of smart-or stimuli-responsive materials, thermoresponsive group of smart polymers consists of the polymers which exhibit rapid, reversible phase transition/phase separation phenomena in response to changes in temperature. The thermosensitive behavior is based on the reversible solubility of the smart polymers caused by increasing temperature above a critical transition temperature (lower critical solution temperature (LCST) or "cloud point") [1][2][3][4][5].…”

Section: Introduction To Poly(n-isopropylacrylamide) (Pnipam) Charactmentioning

confidence: 99%

“…Particularly, poly(N-isopropylacrylamide) (pNIPAm) coatings are of great promise in both basic developmental biology studies and regenerative medicine, as thermoresponsive smart bio-interfaces responding within the physiological temperature range [1][2][3][4][5][6][7][8][9]. Its chemical formula is (C6H11NO) n , having a white solid form.…”

Section: Introduction To Poly(n-isopropylacrylamide) (Pnipam) Charactmentioning

confidence: 99%

See 1 more Smart Citation

Smart Thermoresponsive Surfaces Based on pNIPAm Coatings and Laser Method for Biological Applications

Rusen¹,

Dinca²,

Mustăciosu³

et al. 2017

Modern Technologies for Creating the Thin-Film Systems and Coatings

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Various applications within last decades such as bacterially resistant surfaces, soft robotics, drug delivery systems, sensors and tissue engineering are poised to feature the importance of the ability to control bio-interfacial interactions. An enhanced attention is dedicated to designing smart stimuli-responsive interfaces for DNA, drug delivery, protein and cell based applications. Within this context, the thermoresponsive materials, especially poly(N-isopropylacrylamide) (pNIPAm) have been intensively used in tissue engineering applications for a controlled detachment of proteins and cells with a minimum of invasive effect on protein and cell structural conformation. The properties of smart bio-interfaces can be controlled by its composition and polymer architecture. Therefore, appropriate methods for obtaining controlled coatings are necessary. Laser methods were successfully used in the last decades for obtaining controlled organic and inorganic coatings for various types of applications, from electronics to tissue engineering. Among these, Matrix-Assisted Pulsed Laser Evaporation (MAPLE) technique bring us a step forward to other laser methods by avoiding damage and photochemical decomposition of materials. In this chapter we describe materials and approaches used for design of smart bio-interfaces aimed at controlling protein and cells behavior in vitro, focusing MAPLE method for tuning coatings characteristics in relation with biological response.

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Temperature responsive functional polymeric thin films obtained by matrix assisted pulsed laser evaporation for cells attachment–detachment study

Cited by 26 publications

References 24 publications

Switchable phase transition behavior of thermoresponsive substrates for cell sheet engineering

Switchable phase transition behavior of thermoresponsive substrates for cell sheet engineering

Preparation and Characterization of Thermoresponsive Poly(N-Isopropylacrylamide) for Cell Culture Applications

Smart Thermoresponsive Surfaces Based on pNIPAm Coatings and Laser Method for Biological Applications

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