Temperature Response of<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mmultiscripts><mml:mi>Xe</mml:mi><mml:mprescripts /><mml:none /><mml:mn>129</mml:mn></mml:mmultiscripts></mml:math>Depolarization Transfer and Its Application for Ultrasensitive NMR Detection

Schröder, Leif; Meldrum, Tyler; Smith, Monica A.; Lowery, Thomas J.; Wemmer, David E.; Pines, Alexander

doi:10.1103/physrevlett.100.257603

Cited by 51 publications

(40 citation statements)

References 8 publications

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“…Functionalized biosensors have been detected at concentrations of 10 nm, a sensitivity gain of 4000-fold compared to direct detection. [20,21] With this technique, temperature imaging becomes possible at very low concentrations of the sensor. Such thermometry via indirect detection of the xenon-in-cage chemical shift can be achieved through selective saturation with low bandwidth continuous wave (cw) pulses at different offset frequencies, swept in a range including the xenon-incage center frequency.…”

Section: Indirect Temperature Imagingmentioning

confidence: 99%

“…[20] Chemical shift imaging is a method that maps spectral information, providing a way to display changes in chemical shift with respect to each location in an object or subject of interest. [23] In this way, the xenon-in-cage chemical shift is detected directly in each voxel and can be related to the mean temperature of each voxel.…”

Section: Direct Temperature Imagingmentioning

confidence: 99%

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MRI Thermometry Based on Encapsulated Hyperpolarized Xenon

et al. 2010

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A new approach to MRI thermometry using encapsulated hyperpolarized xenon is demonstrated. The method is based on the temperature dependent chemical shift of hyperpolarized xenon in a cryptophane-A cage. This shift is linear with a slope of 0.29 ppm °C(-1) which is perceptibly higher than the shift of the proton resonance frequency of water (ca. 0.01 ppm °C(-1)) that is currently used for MRI thermometry. Using spectroscopic imaging techniques, we collected temperature maps of a phantom sample that could discriminate by direct NMR detection between temperature differences of 0.1 °C at a sensor concentration of 150 μM. Alternatively, the xenon-in-cage chemical shift was determined by indirect detection using saturation transfer techniques (Hyper-CEST) that allow detection of nanomolar agent concentrations. Thermometry based on hyperpolarized xenon sensors improves the accuracy of currently available MRI thermometry methods, potentially giving rise to biomedical applications of biosensors functionalized for binding to specific target molecules.

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Section: Indirect Temperature Imagingmentioning

confidence: 99%

Section: Direct Temperature Imagingmentioning

confidence: 99%

MRI Thermometry Based on Encapsulated Hyperpolarized Xenon

et al. 2010

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“…2.2-fold. [24] Assuming the slice thickness for the single CEST-acquisition images in Figure 3 b is not more than that for unlocalized acquisition in Figure 1 b, the available xenon signal is spread over ca. 85 pixels for 625 mm in-plane resolution.…”

Section: Angewandte Zuschriftenmentioning

confidence: 99%

Cell Tracking with Caged Xenon: Using Cryptophanes as MRI Reporters upon Cellular Internalization

et al. 2013

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Caged xenon has great potential in overcoming sensitivity limitations for solution-state NMR detection of dilute molecules. However, no application of such a system as a magnetic resonance imaging (MRI) contrast agent has yet been performed with live cells. We demonstrate MRI localization of cells labeled with caged xenon in a packed-bed bioreactor working under perfusion with hyperpolarizedxenon-saturated medium. Xenon hosts enable NMR/MRI experiments with switchable contrast and selectivity for cellassociated versus unbound cages. We present MR images with 10 3 -fold sensitivity enhancement for cell-internalized, dualmode (fluorescence/MRI) xenon hosts at low micromolar concentrations. Our results illustrate the capability of functionalized xenon to act as a highly sensitive cell tracer for MRI detection even without signal averaging. The method will bridge the challenging gap for translation to in vivo studies for the optimization of targeted biosensors and their multiplexing applications.

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“…The method deals with 129 Xe MRI and consists of targeting a cryptophane cage that hosts hyperpolarized 129 Xe (86,87). In aqueous solution, there is a slow exchange between free (the largest fraction) and the cage-sequestered Xe.…”

Section: Targeting Agentsmentioning

confidence: 99%

Encoding the frequency dependence in MRI contrast media: the emerging class of CEST agents

Terreno

Castelli

Aime

2010

Contrast Media & Molecular

112

107

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CEST agents represent a very promising class of MRI contrast media as they encode a frequency dependence that is not like the classical relaxation-based agents. This peculiar property enables novel applications such as the detection of more than one agent in the same MR image as well as the set-up of ratiometric methods for the quantitative assessment of physico-chemical and biological parameters that characterize the micro-environment in which they are distributed. This survey is aimed at providing the reader with the basic properties and the potential of these compounds. Fundamental aspects, such as the theoretical basis of the saturation transfer via chemical exchange, the generation of the CEST contrast, the classification and sensitivity of CEST agents, and some representative examples displaying their potential in the field of MR-molecular imaging, are presented and discussed in detail.

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Temperature Response ofXe129Depolarization Transfer and Its Application for Ultrasensitive NMR Detection

Cited by 51 publications

References 8 publications

MRI Thermometry Based on Encapsulated Hyperpolarized Xenon

MRI Thermometry Based on Encapsulated Hyperpolarized Xenon

Cell Tracking with Caged Xenon: Using Cryptophanes as MRI Reporters upon Cellular Internalization

Encoding the frequency dependence in MRI contrast media: the emerging class of CEST agents

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