Temperature range for growth of Escherichia coli serotype O157:H7 and selected coliforms in E. coli medium

Raghubeer, Errol V.; Matches, Jackr .

doi:10.1128/jcm.28.4.803-805.1990

Cited by 69 publications

(34 citation statements)

References 17 publications

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“…absence of the b-glucuronidase activity (Krishnan et al, 1987)]. Indeed Raghubeer and Matches (1990) reported different growth properties of the pathogen at different temperatures in comparison with other E. coli strains, suggesting that this EHEC strain can also grow at lower temperatures. However, the optimal growth temperature for E. coli O157 and non-O157 strains in nutrient-rich complex media differs only slightly and is between 39°C and 40°C (Gonthier et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Escherichia coli O157 can grow in natural freshwater at low carbon concentrations

Vital

Hammes

Egli

2008

Environmental Microbiology

123

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Whereas much information on the die-off of Escherichia coli in the aquatic environment is available, only few data support its growth under such conditions. We therefore investigated batch growth in microcosms containing different types of sterile freshwater. The water samples were inoculated with low starting cell concentrations of E. coli O157 (3 x 10(3) cells ml(-1)) and growth was followed using nucleic acid staining combined with flow cytometry. We demonstrated that E. coli O157 is able to grow in sterile freshwater at low carbon concentrations, which is against the common view that cell numbers decline over time when added to freshwater samples. A correlation between apparent assimilable organic carbon (AOC(app)) concentration and the final cell concentration reached by E. coli O157 was established (P < 0.01). A considerable fraction of the AOC(app) (34 +/- 13%) was used by E. coli O157 but the numerical cell yield was about five-times lower in comparison with the bacterial AOC-test community, which originated from natural freshwater. On average, the maximum specific growth rate (mu(max)) of E. coli O157 growing in sterile freshwater at 30 degrees C was 0.19 +/- 0.07 h(-1). Batch growth assays at five different temperatures revealed a positive influence of temperature on mu(max) of E. coli O157. The results give new information on the behaviour of this common pathogen in the aquatic environment and contribute to microbial risk assessment in order to prevent spreading of water-borne diseases.

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Section: Discussionmentioning

confidence: 99%

Escherichia coli O157 can grow in natural freshwater at low carbon concentrations

Vital

Hammes

Egli

2008

Environmental Microbiology

123

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“…A strong correlation has been observed between the three cardinal temperatures (T min , T max and T opt ) on several strains of various bacterial species (Rosso et al 1993). Their values for three non-O157 E. coli strains were evaluated between 4á9°C and 11á2°C for T min , 47á3°C and 48á0°C for T max and 40á3°C and 41á3°C for T opt (Rosso et al 1993). The inability of E. coli O157:H7 to grow well, if at all, at 44±45á5°C was reported for trypticase soy broth (TSB) (Doyle and Schoeni 1984) and for E coli medium (Raghubeer and Matches 1990).…”

Section: Introductionmentioning

confidence: 97%

“…T opt (the temperature at which the maximum speci®c growth rate l max equals its optimal value) is an important parameter in predictive microbiology. A strong correlation has been observed between the three cardinal temperatures (T min , T max and T opt ) on several strains of various bacterial species (Rosso et al 1993). Their values for three non-O157 E. coli strains were evaluated between 4á9°C and 11á2°C for T min , 47á3°C and 48á0°C for T max and 40á3°C and 41á3°C for T opt (Rosso et al 1993).…”

Section: Introductionmentioning

confidence: 98%

Optimal growth temperature of O157 and non-O157 Escherichia coli strains

Gonthier

Guérin-Faublée

Tilly

et al. 2001

Lett Appl Microbiol

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Aims: There are several biological characteristics that differ between Escherichia coli O157:H7, a dangerous food-borne pathogen, and the other serotypes of E. coli. Methods and Results: The optimal growth temperatures (T opt ) were determined for 32 E. coli strains, whether each strain belonged to the O157 serotype or not. The mean values of T opt for the O157 and non-O157 groups were 40á2 and 41á2°C, respectively. Signi®cance and Impact of the Study: This difference is statistically signi®cant (P 0á0002) but has no biological implication.

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“…Even in the presence of strong epidemiological evidence of a water-borne source, it has often proved dif®cult to isolate E. coli O157 from water (Ackman et al 1997;Paunio et al 1999). The failure to recover E. coli O157 in these cases may be partially attributed to the limited sensitivity of traditional microbiological methods designed speci®cally for isolating the E. coli O157 from contaminated water sources (Raghubeer and Matches 1990;Rice et al 1992). Given the low infectious dose and the hazardous nature of even dilute E. coli O157 contamination of water, highly sensitive tests are required for detection of this pathogen (Tilden et al 1996).…”

Section: Introductionmentioning

confidence: 99%