Temperature preferences of bacteria isolated from seawater collected in Kandalaksha Bay, White Sea, Russia

Pesciaroli, Chiara; Cupini, Francesco; Selbmann, Laura; Barghini, Paolo; Fenice, Massimiliano

doi:10.1007/s00300-011-1091-1

Cited by 34 publications

(26 citation statements)

References 57 publications

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“…Pseudomonas was the second most dominating genus representing psychrotolerant culturable species. The genus Pseudomonas has been reported to inhabit the lowtemperature environments [29,33]. Species of Bacillus and Pseudomonas have also been found suitable for developing the cold-tolerant bioinoculants for field applications under colder regions in IHR and Tropical Andes due to their plant growth-promoting and biocontrol abilities [31,32].…”

Section: Bioprospection Of Psychrotolerant Bacteriamentioning

confidence: 99%

16S rRNA gene sequencing and MALDI-TOF mass spectrometry based comparative assessment and bioprospection of psychrotolerant bacteria isolated from high altitudes under mountain ecosystem

et al. 2019

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The Himalayan Mountains are placed among the globally recognized biodiversity hot spots. While the Indian Himalayan Region (IHR) has been subjected to extensive studies on plant and animal biodiversity, microbial diversity is now being studied for its bioprospection. The present paper deals with the evaluation of bacterial diversity in high-altitude soil samples from IHR following polyphasic approach including comparison between the MALDI-TOF mass spectrometry and 16S rRNA gene sequencing for species-level identification. Initially, a culture collection of large number of bacterial isolates was established in the laboratory. Performing morphological and biochemical screenings, sixty-one representative isolates were selected for mass spectrometry and gene sequencing. Both the methods emerged with bacterial identification showing maximum number of Bacillus followed by Pseudomonas species. The other frequently isolated strains belonged to the genera Alcaligenes, Carnobacterium, Lysinibacillus, Microbacterium, Paenarthrobacter, Rhodococcus, Serratia and Stenotrophomonas. Although the MALDI-TOF technique appeared to be advantageous as less time-consuming in comparison with 16S rRNA-based method, the discrepancies at species level indicated the limited database of MALDI Biotyper and species complexity in the genera. The remarkable characteristics of the bacterial isolates were their tolerance to wide range of pH and temperature. Their potential to produce industrially valuable enzymes indicated their importance in bioprospection. Accessioning of these bacterial isolates in microbial culture collections is a cautious effort for their availability to conduct advanced research on these cold-adapted bacteria in future.

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Section: Bioprospection Of Psychrotolerant Bacteriamentioning

confidence: 99%

16S rRNA gene sequencing and MALDI-TOF mass spectrometry based comparative assessment and bioprospection of psychrotolerant bacteria isolated from high altitudes under mountain ecosystem

et al. 2019

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“…Some fungal species isolated from Antarctic samples show the ability to produce enzymes at 28.0, 29.0, and 37.0 C, emphasizing the potential to respond to higher growth temperatures. According to Pesciaroli et al [11], microbes capable of growing across a wide spectrum of temperatures are more versatile in relation to environmental changes and able to colonize a wide range of ecological niches in cold environments. These microorganisms are defined as eurythermics and may be useful for industrial processes.…”

Section: Enzymes Produced By Fungi Of Antarctic Originmentioning

confidence: 99%

Cold-adapted enzymes produced by fungi from terrestrial and marine Antarctic environments

Duarte

Santos

Vianna

et al. 2017

Critical Reviews in Biotechnology

108

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Antarctica is the coldest, windiest, and driest continent on Earth. In this sense, microorganisms that inhabit Antarctica environments have to be adapted to harsh conditions. Fungal strains affiliated with Ascomycota and Basidiomycota phyla have been recovered from terrestrial and marine Antarctic samples. They have been used for the bioprospecting of molecules, such as enzymes. Many reports have shown that these microorganisms produce cold-adapted enzymes at low or mild temperatures, including hydrolases (e.g. α-amylase, cellulase, chitinase, glucosidase, invertase, lipase, pectinase, phytase, protease, subtilase, tannase, and xylanase) and oxidoreductases (laccase and superoxide dismutase). Most of these enzymes are extracellular and their production in the laboratory has been carried out mainly under submerged culture conditions. Several studies showed that the cold-adapted enzymes exhibit a wide range in optimal pH (1.0-9.0) and temperature (10.0-70.0 °C). A myriad of methods have been applied for cold-adapted enzyme purification, resulting in purification factors and yields ranging from 1.70 to 1568.00-fold and 0.60 to 86.20%, respectively. Additionally, some fungal cold-adapted enzymes have been cloned and expressed in host organisms. Considering the enzyme-producing ability of microorganisms and the properties of cold-adapted enzymes, fungi recovered from Antarctic environments could be a prolific genetic resource for biotechnological processes (industrial and environmental) carried out at low or mild temperatures.

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“…Pool temperature and salinity were measured by a digital thermometer and a refractometer, respectively. After sampling, 1,000 mL of water were vacuum-filtered on sterile membranes (0.22 µm; Millipore, USA) as reported by Pesciaroli et al (2012). Bacterial cultures were obtained by placing the membranes on Petri dishes that contained Plate Count Agar (PCA; Difco, USA) with different concentrations of marine salt (40,80,160 or 240‰;Difco,USA) and then incubated at 10°C and 25°C.…”

Section: Sample Collectionmentioning

confidence: 99%

Bacteria from the “Saline di Tarquinia” marine salterns reveal very atypical growth profiles with regards to salinity and temperature

Barghini¹,

Pasqualetti

Gorrasi

et al. 2018

Medit. Mar. Sci.

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Several thousand bacterial colonies were isolated from “Saline di Tarquinia” (Italy) marine salterns; after dereplication, thirty-one strains were obtained, confirming the low culturable bacterial diversity attributed to similar hypersaline environments. Strains were identified by 16S ribosomal DNA sequencing and phylogenetic analysis; most strains belonged to the class Gammaproteobacteria, with minorities from Actinobacteria, Bacilli and Alphaproteobacteria. Halomonas and Salinivibrio were predominant genera. To profile growth preferences, strains were cultivated at different salinities and temperatures. Non-metric multi-dimensional scaling (nMDS) analysis on growth data at different salinities identified four groups that were significantly correlated with the ecological categories conventionally used to describe halophiles. The majority were moderate halophiles with optimal growth at 40-80‰ salinity. Some were markedly euryhaline and grew from 40-80‰ to 240-280‰.Very uncommon behaviour was observed for some strains, with a clear optimum growth in the slight to moderate halophilic range, but also the ability to grow without salt. These bacteria could barely be included in the mentioned traditional definitions and we suggest to consider them as “norm-tolerant halophilic”, being somehow in-between the slight halophiles and the halotolerants. As for temperature, nMDS groups were less defined and uncorrelated to ecological categories. Most strains were mesophilic-psychrotolerant or mesophilic, and some psychrotolerants were also recorded. Generally, diffused eurythermism was observed and various strains displayed unusual “flat growth profiles” that showed a broad range of thermal optima. Overall, the majority of strains appeared to be well adapted to the “Saline di Tarquinia” environment and showed great temperature and salinity variations compared to species observed in other salterns.

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Temperature preferences of bacteria isolated from seawater collected in Kandalaksha Bay, White Sea, Russia

Cited by 34 publications

References 57 publications

16S rRNA gene sequencing and MALDI-TOF mass spectrometry based comparative assessment and bioprospection of psychrotolerant bacteria isolated from high altitudes under mountain ecosystem

16S rRNA gene sequencing and MALDI-TOF mass spectrometry based comparative assessment and bioprospection of psychrotolerant bacteria isolated from high altitudes under mountain ecosystem

Cold-adapted enzymes produced by fungi from terrestrial and marine Antarctic environments

Bacteria from the “Saline di Tarquinia” marine salterns reveal very atypical growth profiles with regards to salinity and temperature

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