Temperature Modulation of Integrin-Mediated Cell Adhesion

Rico, Félix; Chu, Calvin; Abdulreda, Midhat H.; Qin, Yujing; Moy, Vincent T.

doi:10.1016/j.bpj.2010.06.037

Cited by 63 publications

(50 citation statements)

References 59 publications

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“…Hence, it stands to reason that cells in our measurements do not display a greater compliance after myosin inhibition. Such hypothesis is supported by the work of Rico et al (56), who varied the adherence of cells by changing the ambient temperature and measured cell compliance using atomic force microscopy. They reported an increase in cell compliance with the loss of adhesion, which is highly connected to the loss of stress fibers in less adherent cells.…”

Section: Discussionmentioning

confidence: 77%

Myosin II Activity Softens Cells in Suspension

Chan

Ekpenyong

Golfier

et al. 2015

Biophysical Journal

102

105

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The cellular cytoskeleton is crucial for many cellular functions such as cell motility and wound healing, as well as other processes that require shape change or force generation. Actin is one cytoskeleton component that regulates cell mechanics. Important properties driving this regulation include the amount of actin, its level of cross-linking, and its coordination with the activity of specific molecular motors like myosin. While studies investigating the contribution of myosin activity to cell mechanics have been performed on cells attached to a substrate, we investigated mechanical properties of cells in suspension.To do this, we used multiple probes for cell mechanics including a microfluidic optical stretcher, a microfluidic microcirculation mimetic, and real-time deformability cytometry. We found that nonadherent blood cells, cells arrested in mitosis, and naturally adherent cells brought into suspension, stiffen and become more solidlike upon myosin inhibition across multiple timescales (milliseconds to minutes). Our results hold across several pharmacological and genetic perturbations targeting myosin. Our findings suggest that myosin II activity contributes to increased whole-cell compliance and fluidity. This finding is contrary to what has been reported for cells attached to a substrate, which stiffen via active myosin driven prestress. Our results establish the importance of myosin II as an active component in modulating suspended cell mechanics, with a functional role distinctly different from that for substrate-adhered cells.

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Section: Discussionmentioning

confidence: 77%

Myosin II Activity Softens Cells in Suspension

Chan

Ekpenyong

Golfier

et al. 2015

Biophysical Journal

102

105

View full text Add to dashboard Cite

show abstract

“…Here all of the cells were cultured with the same cell growth medium to avoid the influence of cell growth medium. Besides, the temperature can also influence the cell mechanics [13,14,36,37]. Rico et al [13] have measured the Young's modulus of THP-1 cells (human monocytic leukemia cell line) at three temperatures (16, 24 and 37°C), showing that the cellular Young's modulus significantly decreased as the temperature increased.…”

Section: Discussionmentioning

confidence: 99%

“…Besides, the temperature can also influence the cell mechanics [13,14,36,37]. Rico et al [13] have measured the Young's modulus of THP-1 cells (human monocytic leukemia cell line) at three temperatures (16, 24 and 37°C), showing that the cellular Young's modulus significantly decreased as the temperature increased. Spedden et al [14] have measured the Young's modulus of neuron cells prepared from embryonic rat at room temperature (25°C) and 37°C, also showing that the increase of temperature can cause the cell softening.…”

Section: Discussionmentioning

confidence: 99%

“…Studies have shown that the obtained Young's moduli of cells by AFM are dependent on several factors, such as the depth of indentation, the loading rate of tip, the position and time of cell poking, and the properties of substrates used for cell growth [12]. In recent years, researchers [13,14] have shown that temperature can also influence the cell stiffness. However, it is still unclear whether the effect of temperature on cell stiffness is a universal phenomenon for all types of cells.…”

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confidence: 99%

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Effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells investigated by atomic force microscopy

Liu

et al. 2015

Sci. China Life Sci.

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Cell mechanics plays an important role in cellular physiological activities. Recent studies have shown that cellular mechanical properties are novel biomarkers for indicating the cell states. In this article, temperature-controllable atomic force microscopy (AFM) was applied to quantitatively investigate the effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells. First, AFM indenting experiments were performed on six types of human cells to investigate the changes of cellular Young's modulus at different temperatures and the results showed that the mechanical responses to the changes of temperature were variable for different types of cancer cells. Second, AFM imaging experiments were performed to observe the morphological changes in living cells at different temperatures and the results showed the significant changes of cell morphology caused by the alterations of temperature. Finally, by co-culturing human cancer cells with human immune cells, the mechanical and morphological changes in cancer cells were investigated. The results showed that the co-culture of cancer cells and immune cells could cause the distinct mechanical changes in cancer cells, but no significant morphological differences were observed. The experimental results improved our understanding of the effects of temperature and cellular interactions on the mechanics and morphology of cancer cells. atomic force microscopy, mechanics, cancer cell, temperature, cellular interactions Citation:Li M, Liu LQ, Xi N, Wang YC, Xiao XB, Zhang WJ. Effects of temperature and cellular interactions on the mechanics and morphology of human cancer cells investigated by atomic force microscopy. Sci China Life Sci, 2015Sci, , 58: 889 -901, doi: 10.1007 The past decade has seen substantial growth in the studies on how changes in the biomechanical and biophysical properties of cells influence, and are influenced by, the onset and progression of many human diseases . Recently, a comprehensive study on human breast tissues has shown that cell mechanics is an effective biomarker that can identify the different stages (normal tissue, benign lesion and invasive cancer) in the process of cancer development [10]. Thus, it is expected that the studies of cell mechanics will have a significant impact on cancer diagnosis and treatment.

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“…The results showed that mild hypothermia could inhibit cells adhesion in flow when the density of adhesion molecules was kept constant. First, Rico and coworkers (41) observed that the work required to detach an atomic force microscope (AFM) cantilever tip functionalized with human ICAM-1-Fc from the cells' surface decreased dramatically from 37ºC to 16ºC. They further found it was due to two main factors.…”

Section: Discussionmentioning

confidence: 99%