Temperature Matters: Bacterial Response to Temperature Change

Moon, Seongjoon; Ham, Soojeong; Jeong, Juwon; Ku, Heechan; Kim, Hyunhee; Lee, Changhan

doi:10.1007/s12275-023-00031-x

Cited by 15 publications

(8 citation statements)

References 161 publications

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“…NIRT-induced photothermal therapy typically demands temperatures exceeding 45 °C for efficient bacterial eradication [45,46]. Structural changes generated by temperature promote gene expression to overcome cellular stress through a heat shock response that attempts to mitigate the effects of high temperatures until eventually the damage to the bacterial cell becomes irreversible [47]. The therapeutic window for NIR diode lasers—where bacterial cell death is achieved without harming the host’s tissue—is quite limited.…”

Section: Discussionmentioning

confidence: 99%

Laser NIR irradiation enhances antimicrobial photodynamic inactivation of biofilms ofStaphylococcus aureus

Mamone,

Tomás,

Di Venosa

et al. 2024

Preprint

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Photodynamic inactivation (PDI) utilizes a photosensitizer (PS) activated by visible light to generate reactive oxygen species (ROS), to kill bacteria. PDI is effective against planktonic microorganisms, but biofilms are less sensitive due to limited PS and oxygen penetration. Near-infrared treatment (NIRT), involve the use of near-infrared light to kill bacteria either via thermal effects or ROS production. Our objective was to enhance S. aureus biofilm's sensitivity to PDI by pre-treating with NIR irradiation before visible light exposure. In an in vitro biofilm model, laser NIRT (980 nm) followed by exposure to PDI, showed a synergistic effect on bacterial viability loss (4-log CFU vs 1-log loss with individual treatments). Interestingly, pre-heating liquid medium had no significant impact on PDI efficacy, suggesting that both thermal and non-thermal effects of NIR may be involved. NIRT increased PS uptake, induced clefts in the biofilm matrix, and released bacterial cells from the biofilm. NIRT induced a transient increase in the temperature to 46°C of in vitro cultures, however under the same conditions, when mice were irradiated, skin temperature rose to 37°. Our findings suggest that NIR irradiation serves as a complementary treatment to PDI, allowing reducing PS concentration, and highlighting its potential as an effective and resource-efficient antibacterial approach.

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Section: Discussionmentioning

confidence: 99%

Laser NIR irradiation enhances antimicrobial photodynamic inactivation of biofilms ofStaphylococcus aureus

Mamone,

Tomás,

Di Venosa

et al. 2024

Preprint

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“…However, these rates were from microcosm-based experiments that did not include other biotic ( e.g. , biofilm and competition) and abiotic (sunlight) factors that can also affect E. coli survivability in-situ ( Korajkic et al, 2014 ; Stocker et al, 2019 ; Petersen & Hubbart, 2020 ; Moon et al, 2023 ). Further studies are therefore needed to understand the role of these factors on E. coli habitat transitions.…”

Section: Discussionmentioning

confidence: 99%

“…The survival of E. coli in aquatic habitats is affected by both biotic and abiotic factors ( Jang et al, 2017 ). For example, biotic factors include biofilm formation and the presence of other microorganisms ( Korajkic et al, 2014 ; Stocker et al, 2019 ), whereas abiotic factors include temperature, pH, salinity, sunlight and nutrient availability ( Petersen & Hubbart, 2020 ; Moon et al, 2023 ). Therefore, seasonal variations with changes in temperature, precipitation and anthropogenic activity could also affect E. coli abundance and their survival.…”

Section: Introductionmentioning

confidence: 99%

Investigating Escherichia coli habitat transition from sediments to water in tropical urban lakes

Liu,

Lee,

Bong

et al. 2024

PeerJ

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Background Escherichia coli is a commonly used faecal indicator bacterium to assess the level of faecal contamination in aquatic habitats. However, extensive studies have reported that sediment acts as a natural reservoir of E. coli in the extraintestinal environment. E. coli can be released from the sediment, and this may lead to overestimating the level of faecal contamination during water quality surveillance. Thus, we aimed to investigate the effects of E. coli habitat transition from sediment to water on its abundance in the water column. Methods This study enumerated the abundance of E. coli in the water and sediment at five urban lakes in the Kuala Lumpur-Petaling Jaya area, state of Selangor, Malaysia. We developed a novel method for measuring habitat transition rate of sediment E. coli to the water column, and evaluated the effects of habitat transition on E. coli abundance in the water column after accounting for its decay in the water column. Results The abundance of E. coli in the sediment ranged from below detection to 12,000 cfu g–1, and was about one order higher than in the water column (1 to 2,300 cfu mL–1). The habitat transition rates ranged from 0.03 to 0.41 h–1. In contrast, the E. coli decay rates ranged from 0.02 to 0.16 h−1. In most cases (>80%), the habitat transition rates were higher than the decay rates in our study. Discussion Our study provided a possible explanation for the persistence of E. coli in tropical lakes. To the best of our knowledge, this is the first quantitative study on habitat transition of E. coli from sediments to water column.

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“…In polar regions, temperature is a factor considerably affecting microbiota [ 74 ] and it plays a crucial role in developing adaptation mechanisms in microbes inhabiting these regions [ 75 ]. Therefore, in this study we investigated the impact of temperature on the total cellular biochemical profile of the Antarctic meltwater bacteria.…”

Section: Discussionmentioning

confidence: 99%

Global biochemical profiling of fast-growing Antarctic bacteria isolated from meltwater ponds by high-throughput FTIR spectroscopy

Akulava,

Tafintseva,

Blazhko

et al. 2024

PLoS ONE

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Fourier transform infrared (FTIR) spectroscopy is a biophysical technique used for non-destructive biochemical profiling of biological samples. It can provide comprehensive information about the total cellular biochemical profile of microbial cells. In this study, FTIR spectroscopy was used to perform biochemical characterization of twenty-nine bacterial strains isolated from the Antarctic meltwater ponds. The bacteria were grown on two forms of brain heart infusion (BHI) medium: agar at six different temperatures (4, 10, 18, 25, 30, and 37°C) and on broth at 18°C. Multivariate data analysis approaches such as principal component analysis (PCA) and correlation analysis were used to study the difference in biochemical profiles induced by the cultivation conditions. The observed results indicated a strong correlation between FTIR spectra and the phylogenetic relationships among the studied bacteria. The most accurate taxonomy-aligned clustering was achieved with bacteria cultivated on agar. Cultivation on two forms of BHI medium provided biochemically different bacterial biomass. The impact of temperature on the total cellular biochemical profile of the studied bacteria was species-specific, however, similarly for all bacteria, lipid spectral region was the least affected while polysaccharide region was the most affected by different temperatures. The biggest temperature-triggered changes of the cell chemistry were detected for bacteria with a wide temperature tolerance such Pseudomonas lundensis strains and Acinetobacter lwoffii BIM B-1558.

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Temperature Matters: Bacterial Response to Temperature Change

Cited by 15 publications

References 161 publications

Laser NIR irradiation enhances antimicrobial photodynamic inactivation of biofilms ofStaphylococcus aureus

Laser NIR irradiation enhances antimicrobial photodynamic inactivation of biofilms ofStaphylococcus aureus

Investigating Escherichia coli habitat transition from sediments to water in tropical urban lakes

Global biochemical profiling of fast-growing Antarctic bacteria isolated from meltwater ponds by high-throughput FTIR spectroscopy

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