Temperature-induced changes in wheat phosphoproteome reveal temperature-regulated interconversion of phosphoforms

Abstract

Wheat (Triticum ssp.) is one of the most important human food sources. However, this crop is very sensitive to temperature changes. Specifically, processes during wheat leaf, flower and seed development and photosynthesis, which all contribute to the yield of this crop, are affected by high temperature. While this has to some extent been investigated on physiological, developmental and molecular levels, very little is known about early signalling events associated with an increase in temperature. Phosphorylation-mediated signalling mechanisms, which are quick and dynamic, are associated with plant growth and development, also under abiotic stress conditions. Therefore, we probed the impact of a short-term increase in temperature on the wheat leaf and spikelet phosphoproteome. The resulting data set provides the scientific community with a first large-scale plant phosphoproteome under the control of higher ambient temperature, which will be valuable for future studies. Our analyses also revealed a core set of common proteins between leaf and spikelet, suggesting some level of conserved regulatory mechanisms. Furthermore, we observed temperature-regulated interconversion of phosphoforms, which likely impacts protein activity.

The Plant PTM Viewer, a central resource for exploring plant protein modifications

“…japonica ), and SUMOylation in Arabidopsis (Rytz et al ., ). Besides the identification of PTMs, differences in overall PTM levels have been assessed, for instance under stress conditions (Jacques et al ., ; Vu et al ., ) or in developmental processes (Wang et al ., ). However, no integrative plant database exists that contains such recently characterized modifications or their dynamics.…”

The Plant PTM Viewer, a central resource exploring plant protein modifications. From site-seeing to protein function

“…japonica ). In addition to the identification of PTMs, several studies assessed differences in overall PTM levels, for instance under stress conditions (Jacques et al, 2015, Vu et al, 2018) or in developmental processes (Wang et al, 2017). However, no integrative plant databases exist that capture such recently characterized modifications or their dynamics.…”

Phosphoproteome Reveals the Lactation Mechanism in Caprine Mammary Gland Tissues in Peak and Late Lactation Stages

“…Protein phosphorylation regulated many cellular processes in eukaryotic cells by post-translational modi cation [10]. Protein phosphorylation revealed the effect of temperature on wheat leaf and spikelet [11] and the importance of protein phosphorylation for different chloroplast functions [12]. Protein phosphorylation had an important role in sperm quality [13] and an important connection with lactation [5,14].…”