We investigated how dwell fatigue loading accelerates the crack propagation in bi-modal Ti6Al4V alloy. A fatigue test was programmed to include displacement holding for only one cycle at several ¦K. We found (1) crack tip strain evolved during the displacement holding, (2) the displacement holding increased fatigue striation spacing, and (3) the strain increment during the displacement holding was linearly correlated with spacing of the displacement-holding-extended striations. These facts indicate that the dwell loading assisted crack opening, which accelerated the crack propagation. Other analyses results and discussion are also presented, in terms of crack propagation mode, crack closure, and dislocation structure.