Temperature impacts on anaerobic biotransformation of LNAPL and concurrent shifts in microbial community structure

Zeman, Natalie Rae; Renno, Maria Irianni; Olson, Mitchell R.; Wilson, L.; Sale, Tom; Long, Susan K. De

doi:10.1007/s10532-014-9682-5

Cited by 17 publications

(27 citation statements)

References 50 publications

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“…Increased subsurface temperatures due to methane oxidation remain a hypothesis requiring further experimentation; however, such a finding would have bearing on kinetics of hydrocarbon degradation and methanogenesis. Heat produced from methane oxidation has been shown to increase methane production, and temperature increases have been shown to increase hydrocarbon degradation rates (Zeman et al 2014). Temperature also has been shown to be a key factor in determining microbial community structure, which may influence degradation rates (Zeman et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Heat produced from methane oxidation has been shown to increase methane production, and temperature increases have been shown to increase hydrocarbon degradation rates (Zeman et al 2014). Temperature also has been shown to be a key factor in determining microbial community structure, which may influence degradation rates (Zeman et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…DNA amplicons were pyrosequenced from the 5′ end. Pyrosequencing data analysis was performed by Research and Testing Laboratory, LLC, as previously described (Zeman et al 2014). or OTU selection and classification were conducted as described by Edgar (2010).…”

Section: Pyrosequencingmentioning

confidence: 99%

“…Historically, the applicability of MNA, or other microbially based treatments, to LNAPL source zones has been considered questionable because biodegradation has been thought to be minimal or non-existent in these zones due to conditions unfavorable for microbial activity (Interstate Technology and Regulatory Council (ITRC) 2009a; Seagren et al 2002). However, research has shown that biodegradation of LNAPL can occur and may be mediated by microorganisms present at the LNAPL-water interface or via the activity of excreted enzymes (ITRC 2009b;Ortega-Calvo and Alexander 1994;Zeman et al 2014). Thus, microbially based remediation technologies are emerging for treatment of LNAPL source zones.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, microbially based remediation technologies are emerging for treatment of LNAPL source zones. To meet treatment goals, such technologies ideally should be developed that lead to relatively rapid biodegradation rates (i.e., that exceed natural attenuation rates) (Zeman et al 2014). However, the fundamental microbiological processes responsible for the degradation of contaminants in LNAPLcontaining zones, the key microorganisms involved, and the effects of site geochemistry on microbial communities are still not well understood.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of bacterial and archaeal communities in depth-resolved zones in an LNAPL body

Irianni-Renno

Akhbari

Olson

et al. 2015

Appl Microbiol Biotechnol

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Advances in our understanding of the microbial ecology at sites impacted by light non-aqueous phase liquids (LNAPLs) are needed to drive development of optimized bioremediation technologies, support longevity models, and develop culture-independent molecular tools. In this study, depth-resolved characterization of geochemical parameters and microbial communities was conducted for a shallow hydrocarbon-impacted aquifer. Four distinct zones were identified based on microbial community structure and geochemical data: (i) an aerobic, low-contaminant mass zone at the top of the vadose zone; (ii) a moderate to high-contaminant mass, low-oxygen to anaerobic transition zone in the middle of the vadose zone; (iii) an anaerobic, high-contaminant mass zone spanning the bottom of the vadose zone and saturated zone; and (iv) an anaerobic, low-contaminant mass zone below the LNAPL body. Evidence suggested that hydrocarbon degradation is mediated by syntrophic fermenters and methanogens in zone III. Upward flux of methane likely contributes to promoting anaerobic conditions in zone II by limiting downward flux of oxygen as methane and oxygen fronts converge at the top of this zone. Observed sulfate gradients and microbial communities suggested that sulfate reduction and methanogenesis both contribute to hydrocarbon degradation in zone IV. Pyrosequencing revealed that Syntrophus- and Methanosaeta-related species dominate bacterial and archaeal communities, respectively, in the LNAPL body below the water table. Observed phylotypes were linked with in situ anaerobic hydrocarbon degradation in LNAPL-impacted soils.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Pyrosequencingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparison of bacterial and archaeal communities in depth-resolved zones in an LNAPL body

Irianni-Renno

Akhbari

Olson

et al. 2015

Appl Microbiol Biotechnol

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Relevance of Candidatus Nitrotoga for nitrite oxidation in technical nitrogen removal systems

Spieck

Wegen

Keuter

2021

Appl Microbiol Biotechnol

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Many biotechnological applications deal with nitrification, one of the main steps of the global nitrogen cycle. The biological oxidation of ammonia to nitrite and further to nitrate is critical to avoid environmental damage and its functioning has to be retained even under adverse conditions. Bacteria performing the second reaction, oxidation of nitrite to nitrate, are fastidious microorganisms that are highly sensitive against disturbances. One important finding with relevance for nitrogen removal systems was the discovery of the mainly cold-adapted Cand. Nitrotoga, whose activity seems to be essential for the recovery of nitrite oxidation in wastewater treatment plants at low temperatures, e.g., during cold seasons. Several new strains of this genus have been recently described and ecophysiologically characterized including genome analyses. With increasing diversity, also mesophilic Cand. Nitrotoga representatives have been detected in activated sludge. This review summarizes the natural distribution and driving forces defining niche separation in artificial nitrification systems. Further critical aspects for the competition with Nitrospira and Nitrobacter are discussed. Knowledge about the physiological capacities and limits of Cand. Nitrotoga can help to define physico-chemical parameters for example in reactor systems that need to be run at low temperatures. Key points • Characterization of the psychrotolerant nitrite oxidizer Cand. Nitrotoga • Comparison of the physiological features of Cand. Nitrotoga with those of other NOB • Identification of beneficial environmental/operational parameters for proliferation

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Advanced methods for RNA recovery from petroleum impacted soils

2021

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Microbially-mediated hydrocarbon degradation is well documented. However, how these microbial processes occur in complex subsurface petroleum impacted systems remains unclear, and this knowledge is needed to guide technologies to enhance microbial degradation effectively. Analysis of RNA derived from soils impacted by petroleum liquids would allow for analysis of active microbial communities, and a deeper understanding of the dynamic biochemistry occurring during site remediation. However, RNA analysis in soils impacted with petroleum liquids is challenging due to: (A) RNA being inherently unstable, and (B) petroleum impacted soils containing problematic levels of polymerase chain reaction (PCR) inhibitors that must be removed to yield high-purity RNA for downstream analysis. A previously published soil wash pretreatment step and a commercially available DNA extraction kit protocol were combined and modified to be able to purify RNA from soils containing petroleum liquids. A key modification involved reformulation of the pretreatment solution via replacing water as the diluent with a commercially-available RNA preservation solution. Methods were developed and demonstrated using cryogenically preserved soils from three former petroleum refineries. Results showed the new soil washing approach had no adverse effects on RNA recovery but did improve RNA quality, by PCR inhibitor removal, which in turn allows for characterization of active microbial communities present in petroleum impacted soils. In summary, our method for extracting RNA from petroleum-impacted soils provides a promising new tool for resolving metabolic processes at sites as they progress toward restoration via natural and/or engineered remediation.

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Temperature impacts on anaerobic biotransformation of LNAPL and concurrent shifts in microbial community structure

Cited by 17 publications

References 50 publications

Comparison of bacterial and archaeal communities in depth-resolved zones in an LNAPL body

Comparison of bacterial and archaeal communities in depth-resolved zones in an LNAPL body

Relevance of Candidatus Nitrotoga for nitrite oxidation in technical nitrogen removal systems

Advanced methods for RNA recovery from petroleum impacted soils

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