Temperature evolution of hydration shells in solid DNA didecyldimethylammonium chloride complex studied by 1H NMR spectroscopy

Nizioł, Jacek; Nowak, Piotr; Kobierski, Jan; Harańczyk, H.

doi:10.1016/j.eurpolymj.2015.02.037

Cited by 8 publications

(6 citation statements)

References 47 publications

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“…The 1 H-NMR immobilized proton signal component coming from the tissues of anhydrobiotic larva does not differ much from the one detected for other microheterogenous solids, and particularly, for other deeply dehydrated biological systems, eg. DNA-surfactant complexes 53 , photosynthetic membranes 54 , or for anhydrobiotic living organisms, e.g. Antarctic lichenized fungi 55 – 57 .…”

Section: Discussionmentioning

confidence: 99%

Rehydration of the sleeping chironomid, Polypedilum vanderplanki Hinton, 1951 larvae from cryptobiotic state up to full physiological hydration (Diptera: Chironomidae)

Knutelski

Harańczyk

Nowak

et al. 2022

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During desiccation the Polypedilum vanderplanki larva loses 97% of its body water, resulting in the shutdown of all metabolic and physiological processes. The larvae are able to resume active life when rehydrated. As dehydration process has already been largely understood, rehydration mechanisms are still poorly recognized. X-ray microtomograms and electron scanning microscopy images recorded during the hydration showed that the volume of the larva's head hardly changes, while the remaining parts of the body increase in volume. In the 1H-NMR spectrum, as recorded for active larvae, component characteristic of solid state matter is absent. The spectrum is superposition of components coming from tightly and loosely bound water fraction, as well as from lipids. The value of the c coefficient (0.66 ± 0.02) of the allometric function describing the hydration models means that the increase in the volume of rehydrated larvae over time is linear. The initial phase of hydration does not depend on the chemical composition of water, but the amount of ions affects the further process and the rate of return of larva’s to active life. Diffusion and ion channels play a major role in the permeability of water through the larva's body integument.

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Section: Discussionmentioning

confidence: 99%

Rehydration of the sleeping chironomid, Polypedilum vanderplanki Hinton, 1951 larvae from cryptobiotic state up to full physiological hydration (Diptera: Chironomidae)

Knutelski

Harańczyk

Nowak

et al. 2022

Sci Rep

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“…Among them are 1 H-NMR relaxometry, 1 H-NMR spectroscopy, and sorption isotherm analysis. They allow the analysis of molecular dynamics of water molecules and differentiation of several fractions of residual water present in a cryptobiotic organism (in dehydrated lichenized fungi) (Harańczyk et al 2006(Harańczyk et al , 2008(Harańczyk et al , 2009(Harańczyk et al , 2012b, in freeze dried photosynthetic membranes (Harańczyk et al 2015), or in other extremely dry biological systems like DNA-based conducting polymers (Nizioł et al 2015).…”

Section: Introductionmentioning

confidence: 99%

A comparative analysis of gaseous phase hydration properties of two lichenized fungi: Niebla tigrina (Follman) Rundel & Bowler from Atacama Desert and Umbilicaria antarctica Frey & I. M. Lamb from Robert Island, Southern Shetlands Archipelago, maritime Antarctica

et al. 2021

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Gaseous phase hydration properties for thalli of Niebla tigrina from Atacama Desert, and for Umbilicaria antarctica from Isla Robert, maritime Antarctica, were analyzed using 1H-NMR relaxometry, spectroscopy, and sorption isotherm analysis. The molecular dynamics of residual water was monitored to distinguish the sequential binding very tightly, tightly, and loosely bound water fractions. These two species differ in hydration kinetics faster for Desert N. tigrina [A1 = 0.51(4); t1 = 0.51(5) h, t2 = 15.0(1.9) h; total 0.7 for p/p0 = 100%], compared to Antarctic U. antarctica [A1 = 0.082(6), t1 = 2.4(2) h, t2 = [26.9(2.7)] h, total 0.6 for p/p0 = 100%] from humid polar area. The 1H-NMR measurements distinguish signal from tightly bound water, and two signals from loosely bound water, with different chemical shifts higher for U. antarctica than for N. tigrina. Both lichen species contain different amounts of water-soluble solid fraction. For U. antarctica, the saturation concentration of water soluble solid fraction, cs = 0.55(9), and the dissolution effect is detected at least up to Δm/m0 = 0.7, whereas for N. tigrina with the similar saturation concentration, cs = 053(4), this fraction is detected up to the threshold hydration level equal to ΔM/m0 = 0.3 only.

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“…The first shell, referred to as tightly bound water, consists of molecules directly bound via hydrogen bonds to hydrophilic sites on the DNA helix. To the other one, known as loosely bound water, belong water molecules attached to tightly bound waters [11]. These waters can be the source of uncontrolled ionic conduction that may occur extremely detrimental for performance of organic electronics devices.…”

Section: Introductionmentioning

confidence: 99%

Thermal degradation of biological DNA studied by dielectric spectroscopy

et al. 2019

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Dielectric spectroscopy was tested as an alternative tool to study degradation of deoxyribonucleic acid (DNA) in its solid form. The specimens, prepared from biological DNA, were periodically heated and cooled according to a programmed scheme. Simultaneously, their dielectric parameters (permittivity and dielectric loss) were monitored as function of frequency and temperature. The analysis of Bode plots allowed to determine the upper limit of thermal stability of solid DNA at 120 � C, because heating at higher temperatures resulted in irreversible changes. These changes were identified as denaturation by gel electrophoresis and UV-vis absorption methods.

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Temperature evolution of hydration shells in solid DNA didecyldimethylammonium chloride complex studied by 1H NMR spectroscopy

Cited by 8 publications

References 47 publications

Rehydration of the sleeping chironomid, Polypedilum vanderplanki Hinton, 1951 larvae from cryptobiotic state up to full physiological hydration (Diptera: Chironomidae)

Rehydration of the sleeping chironomid, Polypedilum vanderplanki Hinton, 1951 larvae from cryptobiotic state up to full physiological hydration (Diptera: Chironomidae)

A comparative analysis of gaseous phase hydration properties of two lichenized fungi: Niebla tigrina (Follman) Rundel & Bowler from Atacama Desert and Umbilicaria antarctica Frey & I. M. Lamb from Robert Island, Southern Shetlands Archipelago, maritime Antarctica

Thermal degradation of biological DNA studied by dielectric spectroscopy

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