Temperature Effects on Pea Plants Probed by Simultaneous Measurements of the Kinetics of Prompt Fluorescence, Delayed Fluorescence and Modulated 820 nm Reflection

Oukarroum, Abdallah; Goltsev, Vasilij; Strasser, Reto J.

doi:10.1371/journal.pone.0059433

Cited by 104 publications

(90 citation statements)

References 54 publications

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“…According to several published reports, 19,27 leaf heating in hot water can be used for fast damage of photosystems. We used this method in our experiments, the schema of which is shown in Fig.…”

Section: Methodsmentioning

confidence: 99%

Variation potential propagation decreases heat-related damage of pea photosystem I by 2 different pathways

Surova

Sherstneva

Vodeneev

et al. 2016

Plant Signaling & Behavior

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Local burning is known to generate and propagate variation potential (VP) in plants. VP affects different physiological processes, including reducing heat-related damage to photosystem I (PSI). We investigated mechanisms of the process. Photosynthesis parameters were measured with Dual-PAM-100 and GFS-3000. VP was induced by burning the first mature leaf and then waiting 5, 10, 15, or 20 min to initiate heating of the second mature leaf. Photosystems activities in the second leaf were investigated at 15 and 135 min after heating. In the absence of VP induction, when incubation in hot water (5 min) was used for heating the intact second leaf, PSI and PSII activities decreased after incubation at both exposure temperatures (45 C and 50 C). When local burning of the first leaf induced VP propagation into the second leaf, reduced photosynthesis (PSI) was observed. Arrival of VP in the second leaf prior to hot water incubation at 50 C decreased heating-induced suppression of PSI activity when measured 15 and 135 min later. Dependence of PSI activity on the time interval (5, 10, 15, or 20 min) between VP induction and heating of the second leaf was dissimilar at 15 and 135 min. Heat-induced suppression of PSII activity in the second leaf was stimulated after VP induction. In contrast, the effect of VP on PSI and PSII damage was weak when leaf 2 was heated at 45 C. VP-induced decrease of PSI activity suppression at 15 min after heating was correlated with stimulation of PSII activity suppression, but increase of PSI activity at 135 min after heating was not related to PSII activity. Thus, our results suggest the possibility of 2 different pathways of VPinduced decrease of heat-related PSI damage.Abbreviations: A CO2 , CO 2 assimilation rate; E, surface potential; PSI, photosystem I; PSII, hotosystem II; VP, variation potential; L PSI , photosystem I effective quantum yield; L PSII , photosystem II effective quantum yield; L PSI 0 , photosystem I quantum yield after 10-s illumination by far-red light; L PSII 0 , photosystem II maximum quantum yield.

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Section: Methodsmentioning

confidence: 99%

Variation potential propagation decreases heat-related damage of pea photosystem I by 2 different pathways

Surova

Sherstneva

Vodeneev

et al. 2016

Plant Signaling & Behavior

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“…Two phases can be seen on the FD induction curve, a phase that occurs for up to around 300 ms, including the peaks at I 1 (7 ms) and I 2 (50 ms), and a slow phase that can last for several minutes (GOLTSEV et al, 2009;OUKARROUN;STRASSER, 2013;STRASSER et al, 2010).…”

Section: Analysis Of the Kinetics Of Delayed Fluorescence (Df)mentioning

confidence: 99%

“…However, just as the chlorophylls of the PSII emit light as FT, they also emit another type of light, known as delayed fluorescence (DF), as a result of the reversal of charge separation followed by the rapid transfer of P 680 * excitation energy to the chlorophylls of the antenna system (GOLTSEV et al, 2009;STRASSER, 2013;STRASSER et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…To distinguish the two types of light emission, FT is measured at the same time as illumination, while DF is measured after the actinic light is turned off, in alternating cycles of light and dark (GOLTSEV et al, 2009;STRASSER, 2013). According to Grabolle and Dau (2005), the similarity between the spectral emissions of FT and DF shows that the emitted photon is the result of the radioactive deactivation of the excited state of the PSII Chl.…”

Section: Introductionmentioning

confidence: 99%

“…It has been proposed that the kinetics of modulated reflection at 820 nm (MR 820 ) may reflect PSI activity (STRASSER et al, 2010;YAN et al, 2012), indicating alterations in the redox state of the PC and of the PSI (P 700 ) reaction centre (RC) (OUKARROUM; GOLTSEV; STRASSER, 2013;YAN et al, 2013). Strasser et al (2010) emphasize that simultaneous measurements of the FT, DF and MR 820 are essential for complete correlation of the information on the different dominions of the ETC.…”

Section: Introductionmentioning

confidence: 99%

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Chlorophyll fluorescence in the evaluation of photosynthetic electron transport chain inhibitors in the pea

Farias

Martinazzo

Bacarin

2016

Revista Ciência Agronômica

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-The study aimed to evaluate the behavior of the chair of photosynthetic electron transport in the presence of DCMU and atrazine in detached leaves of pea through simultaneous measurements of the kinetics of fluorescence transient, fluorescence delayed and modulated reflection at 820 nm. The petioles of the leaves were immersed for two hours in solution of inhibitors at concentrations of 0 (control), 25, 50, 100, 250 and 500 M. Measurements of the kinetics of fluorescence transient and fluorescence delayed and modulated reflection at 820 nm were recorded simultaneously by the M-PEA fluorometer. Simultaneous measurement of fluorescence transient, fluorescence delayed and modulated 820 nm reflection is important for assessments of the photosynthetic electron transport chain activity tool. The use of specific inhibitors of the electron transport chain allows you to collect and correlate a lot of information about the effect of different inhibitors at specific points in the photosynthetic electron transport chain. DCMU and atrazine are inhibitors of photosystem II and the concentration of 500 mM affects more strongly the flow of photosynthetic electrons. RESUMO -O trabalho teve como objetivo avaliar o comportamento da cadeia de transporte de elétrons fotossintética na presença de DCMU e atrazina, em folhas destacadas de ervilha, através de medidas simultâneas das cinéticas da fluorescência transiente, decaída da fluorescência da clorofila a e reflexão modulada a 820 nm. O pecíolo das folhas foi imerso por duas horas em solução de inibidores nas concentrações de 0 (controle), 25, 50, 100, 250 e 500 µM. Medidas da cinética da fluorescência transiente, da decaída da fluorescência e reflexão modulada a 820 nm foram gravadas, simultaneamente, pelo fluorômetro M-PEA. A medida simultânea da fluorescência transiente, decaída da fluorescência e reflexão modulada a 820 nm é ferramenta importante para avaliações da atividade da cadeia de transporte de elétrons fotossintética. O uso de inibidores específicos da cadeia de transporte de elétrons permite coletar e correlacionar uma série de informações sobre o efeito de diferentes inibidores em pontos específicos na cadeia de transporte de elétrons fotossintético. DCMU e atrazina são inibidores do fotossistema II e a concentração de 500 µM afeta de maneira mais acentuada o fluxo de elétrons fotossintético. Palavras-chave:Pisum sativum L.. Fluorescência transiente. Decaída da fluorescência. DCMU. Atrazina.

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Eco‐physiological responses of Calamagrostis epigejos L (Roth) and Solidago gigantea Aition to complex environmental stresses in coal‐mine spoil heaps

et al. 2021

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We present a study of short and long‐term responses of photosynthetic apparatus and anti‐oxidant capacity to complex abiotic stresses of Calamagrostis epigejos and Solidago gigantea commonly occurring in seminatural habitats and novel ecosystems of coal‐mine spoil heaps. Drought/salinity and elevated temperature were dominant abiotic stressors triggering both species‐ and habitat‐specific responses, confirmed by ChlF induction curves analysis. Although in both species a decrease in net photosynthetic rate (A) and transpiration rate (E) in spoil heap were observed, Ce showed higher (A) on both habitats in comparison to Sg. Moreover, we found higher H2O2 concentration in Sg leaves as compared to Ce leaves, large differences in catalase (CAT) activity and the reverse pattern of lipid peroxidation in Sg and Ce populations, suggesting species‐specific differences in antioxidative mechanisms. The Sg individuals developed structural and functional adaptations to protect PSA against drought/salinity stresses (lower leaf chlorophyll, higher flavonoids content, ChlF parameters: Vi, Vj, dVG/dto). The Ce populations have higher values of JIP parameters related to the electron transfer site within PSI. Mechanisms of plant species adaptation to industrial areas are crucial for species selection and planning effective reclamation of them. In novel ecosystems of spoil heaps both species responded differently to complex abiotic stresses in comparison to seminatural ones that enable them to gain success on both sites. They can spontaneously colonize such areas, create permanent plant cover, and produce large amounts of biomass. Further research on plant traits response and adaptation to complex environmental stresses on industrial habitats are needed.

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Temperature Effects on Pea Plants Probed by Simultaneous Measurements of the Kinetics of Prompt Fluorescence, Delayed Fluorescence and Modulated 820 nm Reflection

Cited by 104 publications

References 54 publications

Variation potential propagation decreases heat-related damage of pea photosystem I by 2 different pathways

Variation potential propagation decreases heat-related damage of pea photosystem I by 2 different pathways

Chlorophyll fluorescence in the evaluation of photosynthetic electron transport chain inhibitors in the pea

Eco‐physiological responses of Calamagrostis epigejos L (Roth) and Solidago gigantea Aition to complex environmental stresses in coal‐mine spoil heaps

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