Temperature Effect Study on Growth and Survival of PathogenicVibrio parahaemolyticusin Jinjiang Oyster(Crassostrea rivularis)with Rapid Count Method

Abstract

The growth of Vibrio parahaemolyticus (V. parahaemolyticus) in oysters during postharvest storage increases the possibility of its infection in humans. In this work, to investigate the growth or survival profiles in different media, pathogenic V. parahaemolyticus in APW, Jinjiang oyster (JO, Crassostrea rivularis) slurry, and live JO were studied under different temperatures. All the strain populations were counted through our double-layer agar plate (DLAP) method. In APW, the pathogenic V. parahaemolyticus showed continuous growth under 15, 25, and 35°C, while a decline in behavior was displayed under 5°C. The similar survival trend of pathogenic V. parahaemolyticus in JO slurry and live JO was observed under 5, 25, and 35°C, except the delayed growth or decline profile compared to APW. Under 15°C, they displayed decline and growth profile in JO slurry and live JO, respectively. These results indicate the different sensitivity of pathogenic V. parahaemolyticus in these matrices to temperature variation. Furthermore, nonpathogenic V. parahaemolyticus displayed little difference in survival profiles when inoculated in live JO under corresponding temperatures. The results indicate that inhibition or promotion effect could be regulated under different storage temperature for both pathogenic and nonpathogenic strains. Besides, the DLAP method showed the obvious quickness and efficiency during the bacteria count.

Effect of sunlight and salinity on the survival of pathogenic and non‐pathogenic strains of Vibrio parahaemolyticus in water microcosms

“…Our results are in agreement with previous studies that focused on the effect of light radiation on some bacterial species, such as Escherichia coli, Salmonella, Campylobacter, and Pseudomonas, but with a difference in the survival capacities (Giannakis et al, 2015;Jozi c et al, 2014;Lipovsky et al, 2010;Orruño et al, 2017). Generally, the bacterial response to light exposure depends on several factors, such as the type and intensity of lighting (Davies & Evison, 1991;Jozi c & Šoli c, 2017;Wang et al, 2018), the resistance ability of each group of bacteria (Jozi c et al, 2014;Maraccini et al, 2016), and the oxygen concentration in the irradiation medium (Baez & Shiloach, 2014;Oppezzo et al, 2011). In our experience, oxygen saturation in each microcosm was around 80%.…”

Occurrence and antibiotic resistance of Vibrio parahaemolyticus isolated from the Tunisian coastal seawater

“…The geographical coordinates of each station were obtained by a Garmin 60 GPS. Water samples were collected between 9 and 10 pm at 0.3 m depth in a 500 ml sterile glass bottle and then conserved in the darkness at 10 °C approximately, which is considered a non-stressful condition for Vibrio species (Shen et al 2009;Wang et al 2018). According to the proximity of the sampling stations to the laboratory, samples were processed within 7 min to 20 h after sampling.…”

“…Some V. parahaemolyticus strains are pathogenic to humans and were responsible for 25% of seafood-borne diseases (Martinez-Urtaza et al 2013;Tran et al 2018). This virulence was associated especially with the production of thermostable direct haemolysin (tdh) and/or thermostable-related haemolysin (trh) (Wang et al 2018), also known as the Kanagawa phenomenon (KP) (Leoni et al 2016). Nevertheless, virulence was reported in some negative tdh and trh strains (Ottaviani et al 2012;Chung et al 2016).…”

Characterization and growth conditions of Vibrio parahaemolyticus strains with different virulence degrees that cause acute hepatopancreatic necrosis disease in Litopenaeus vannamei

“…The minimum growth rate of clinical Vp was 8.3°C (Miles, Ross, Olley, & McMeekin, ). Vp growth, as for most vibrios, is dependent on temperature, and the organism can grow well between 15 and 30°C (Thompson et al, ); in temperatures of under 15°C, Vp displayed decline the growth profile (Wang et al, ). In this study, temperature was strongly associated with vibrio densities; all strains grew better at 30 and 37°C, but only AHPND+ strains reached the highest density, over the infective threshold, and only these strains grow at 44°C.…”