Temperature effect in physicochemical and bioactive behavior of biogenic hydroxyapatite obtained from porcine bones

Abstract

Biogenic hydroxyapatite (BHAp) is a widely used material in the biomedical area due to its similarities with the bone tissue mineral phase. Several works have been spotlighted on the thermal behavior of bone. However, little research has focused on determining the influence of calcination temperature in the physicochemical and bioactive properties of BHAp. In this work, a study of the physicochemical properties’ changes and bioactive response of BHAp produced from porcine femur bones using calcination temperatures between 900 to 1200 °C was conducted. The samples’ structural, morphological, and compositional changes were determined using XRD, SEM, and FTIR techniques. XRD results identified three temperature ranges, in which there are structural changes in BHAp samples and the presence of additional phases. Moreover, FTIR results corroborated that B-type substitution is promoted by increasing the heat treatment temperature. Likewise, samples were immersed in a simulated biological fluid (SBF), following the methodology described by Kokubo and using ISO 23317:2014 standard, for 3 and 7 days. FTIR and SEM results determined that the highest reaction velocity was reached for samples above 1000 °C, due to intensity increasing of phosphate and carbonate bands and bone-like apatite morphologies, compared to other temperatures evaluated.

Hydroxyapatite as a Material for Transplantology: Clinical Experience and Prospects of Application

“…These data, in addition to the high feasibility of using this raw material, give an idea of the high degree of biomimetic potential of the obtained hydroxyapatite in comparison with the mineral phase of human bone [165]. This is supported by the data that testify in favor of similarity of leading morphological and microarchitectural parameters of treated hydroxyapatite, including agglomeration of hydroxyapatite particles as a result of high-temperature treatment [183]. For example, the specific surface area of synthesized hydroxyapatite is in the range of values typical of native bone tissue [184].…”

Microwave-Assisted Hydrothermal Treatment of Multifunctional Substituted Hydroxyapatite with Prospective Applications in Bone Regeneration

“…Hydroxyapatite (HAp, Ca 10 (PO 4 ) 6 (OH) 2 ) is among the most promising crystalline calcium phosphate considered to be biomaterial due to its chemical resemblance to the inorganic part of human hard tissues such as bone and teeth [2][3][4]. Stoichiometric and pure HAp crystallizes in the monoclinic system, and it most often crystallizes in the hexagonal system at over 250 • C and thermally decomposes at temperatures between 800 and 1200 • C [5,6]. Bone hydroxyapatite has a small dimensional structure, about 25 nm wide, 50 nm long, and 10 nm high [7].…”

In Situ Facile Synthesis of Low-Cost Biogenic Eggshell-Derived Nanohydroxyapatite/Chitosan Biocomposites for Orthopedic Implant Applications

“…In general, the interactions between the artificial implants and the surrounding bone tissues are determined by the surface properties of the implants [ 4 ]. The apatite of natural bone is nonstoichiometric hydroxyapatite because the bone contains various elements with different concentrations [ 5 ]. The long-term interaction of the stable synthetic implant in the surrounding tissues minimizes the ablation of post-operative implant failures, which may occur due to aseptic loosening and infections [ 6 ].…”