Temperature drives the assembly of endophytic communities' seasonal succession

Abstract

Endophytic microorganisms asymptomatically colonise plant tissues. Exploring the assembly dynamics of bacterial endophytic communities is essential to understand the functioning of the plant holobiont and to optimise their possible use as biopesticides or plant biostimulants. The variation in endophytic communities in above and below-ground organs in Vitis vinifera in the field were studied. To understand the specific effect of temperature on endophytic communities, a separate experiment was set up where grapevine cuttings were grown under controlled conditions at three different temperatures. The findings revealed the succession of endophytic communities over the year. Endophytic communities of roots and stems differ in terms of composition and dynamic response to temperature. Noticeably, compositional differences during the seasons affected bacterial taxa more in stems than in roots, suggesting that roots offer a more stable and less easily perturbed environment. Correlation abundance networks showed that the presence of several taxa (including Bradyrhizobium, Burkholderia, Dyella, Mesorhizobium, Propionibacterium and Ralstonia) is linked in both the field and the greenhouse.

Century long fertilization reduces stochasticity controlling grassland microbial community succession

“…This pattern of increasing stochasticity might be due to the cumulative effects of neutral dispersal and random drift over time and/or decrease in cumulative deterministic linkages under unidirectional selection across a long time scale 56 . Furthermore, we found that fertilization reduced the stochasticity of functional structure turnover across the century, corresponding to previous findings that high determinism drove phylogenetic turnovers under selective pressures 13,15,17 . Similarly, we recently reported that a 6-year warming significantly decreased the stochasticity of phylogenetic succession in grassland bacterial communities 8 .…”

“…For instance, under resource-rich and low-stress environments, microbial succession was found to be governed primarily by stochastic processes (e.g., dispersal and drift) 12–14 . In contrast, under environments with selective pressures, microbial succession was reported to be controlled by deterministic abiotic filtering, including temperature 15,16 , pH 13 , resource type 7,17 , nutrient availability 18,19 , and/or biotic interactions 20,21 .…”

Tissue-Specific and Geographical Variation in Endophytic Fungi of Ageratina adenophora and Fungal Associations With the Environment

“…Moreover, there was a higher percentage of shared fungi in aboveground tissues and canopy air than in roots and rhizosphere soils (Figure 10 and Supplementary Figure S3), indicating that the environmental inoculation sources played a more important role in the fungal assembly of aboveground parts than in that of the belowground parts. Interestingly, even the assemblage of fungal communities in plant tissues may be a compromise driven by both functional requirements for plant growth (Torres-Cortes et al, 2018) and local environmental conditions, e.g., the temperature (Campisano et al, 2017); the former may weight out the latter because we found that the fungal similarity within tissues was much higher than that between tissues on a relatively large geographical scale (Figure 3). Within a host, some studies have demonstrated endophytic bacterial migration through tissues (Chi et al, 2005;Tharek et al, 2011).…”

Apple endophyte community is shaped by tissue type, cultivar and site and has members with biocontrol potential againstNeonectria ditissima

“…Tissue type, cultivar and site influenced the composition of endophyte communities in apple shoots and this has been shown for many plant species throughout the world including, by tissue type, for Viscum album , Pinus sylvestris in Germany and Austria (Peršoh ) and Vitis vinifera in Italy (Campisano et al ), by cultivar for potato ( Solanum tuberosum ) in the Netherlands (Andreote et al ) and grapevine ( Vitis vinifera ) in China (Jayawardena et al ), and by site for Arabidopsis thaliana and poplar ( Populus × euramericana ) in Spain (Knief et al ; Martín‐García et al ). This result suggested that isolating endophytes from target tissue types and cultivars may be important for selecting potential biocontrol agents.…”