Temperature dependent self-organization of DMPC membranes promoted by intermediate amounts of the saponin aescin

Sreij, Ramsia; Dargel, Carina; Hannappel, Yvonne; Jestin, Jacques; Prévost, Sylvain; Dattani, Rajeev; Wrede, Oliver; Hellweg, Thomas

doi:10.1016/j.bbamem.2019.01.015

Cited by 26 publications

(63 citation statements)

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“…Effects directly related to the interaction of aescin with the membranes of the cells contained in the veins have not been mentioned in this context so far. Nevertheless, it is known that aescin interacts with lipid membranes [18,[39][40][41], but the physico-chemical interaction on the molecular scale is still not fully understood. However, the behavior of aescin in solution, especially above the cmc, is essential to elucidate the structure-activity relationship with lipid membranes and the mechanism of action of aescin.…”

Section: Introductionmentioning

confidence: 99%

Self-Assembly of the Bio-Surfactant Aescin in Solution: A Small-Angle X-ray Scattering and Fluorescence Study

Dargel

Geisler

Hannappel

et al. 2019

Colloids and Interfaces

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This work investigates the temperature-dependent micelle formation as well as the micellar structure of the saponin aescin. The critical micelle concentration ( c m c ) of aescin is determined from the concentration-dependent autofluorescence (AF) of aescin. Values between c m c aescin , AF (10 ∘ C) = 0.38 ± 0.09 mM and c m c aescin , AF (50 ∘ C) = 0.32 ± 0.13 mM were obtained. The significance of this method is verified by tensiometry measurements. The value determined from this method is within the experimental error identical with values obtained from autofluorescence ( c m c aescin , T ( WP ) (23 ∘ C) = 0.33 ± 0.02 mM). The structure of the aescin micelles was investigated by small-angle X-ray scattering (SAXS) at 10 and 40 ∘ C. At low temperature, the aescin micelles are rod-like, whereas at high temperature the structure is ellipsoidal. The radii of gyration were determined to ≈31 Å (rods) and ≈21 Å (ellipsoid). The rod-like shape of the aescin micelles at low temperature was confirmed by transmission electron microscopy (TEM). All investigations were performed at a constant pH of 7.4, because the acidic aescin has the ability to lower the pH value in aqueous solution.

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Section: Introductionmentioning

confidence: 99%

Self-Assembly of the Bio-Surfactant Aescin in Solution: A Small-Angle X-ray Scattering and Fluorescence Study

Dargel

Geisler

Hannappel

et al. 2019

Colloids and Interfaces

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“…At a lower β-aescin-to-DMPC ratio, larger structures are stabilized and vice versa. Moreover, there are indications that the bicelles undergo a temperature-dependent phase transition into large vesicles and stacked bilayers [48,49], similar to phospholipid-bile salt systems [56]. In addition to the DMPC-β-aescin system, solubilization of a 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) membrane by β-aescin in the lipids gel-phase was reported in a study of de Groot et al [39].…”

Section: β-Aescin Interaction With Bare Lipid Model Membranesmentioning

confidence: 91%

“…The interaction of β-aescin with bare lipid model membranes from the physicochemical perspective is subject of works published over the last 2 years by Sreij et al [46][47][48] and Geisler et al [49]. The work is done exemplarily on phospholipid bilayers composed of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) at constant environmental conditions, i.e., in 50 mM phosphate buffer at physiological p(H)≈ p(D) of about 7.4 and a DMPC mass concentration of w(DMPC) = 15 mg/mL.…”

Section: β-Aescin Interaction With Bare Lipid Model Membranesmentioning

confidence: 99%

“…At around 6 mol% of the saponin the signal broadens significantly and is only weakly visible indicating different structures in the samples. A study devoted to the "mid" concentration regime between 1 and 7 mol% [48] (region II) showed that an increasing β-aescin concentration leads to aggregated SUVs which start to deform and solubilize at concentrations around cmc(β-aescin). The diverse structures simultaneously present in this regime are visualized by cryogenic transmission electron microscopy (cryo-TEM) for a sample with 4 mol% β-aescin ( Figure 11).…”

Section: β-Aescin Interaction With Bare Lipid Model Membranesmentioning

confidence: 99%

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The Biosurfactant β-Aescin: A Review on the Physico-Chemical Properties and Its Interaction with Lipid Model Membranes and Langmuir Monolayers

2019

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This review discusses recent progress in physicochemical understanding of the action of the saponin β -aescin (also called β -escin), the biologically active component in the seeds of the horse chestnut tree Aesculus hippocastanum. β -Aescin is used in pharmacological and cosmetic applications showing strong surface activity. In this review, we outline the most important findings describing the behavior of β -aescin in solution (e.g., critical micelle concentration ( c m c ) and micelle shape) and special physicochemical properties of adsorbed β -aescin monolayers at the air–water and oil–water interface. Such monolayers were found to posses very special viscoelastic properties. The presentation of the experimental findings is complemented by discussing recent molecular dynamics simulations. These simulations do not only quantify the predominant interactions in adsorbed monolayers but also highlight the different behavior of neutral and ionized β -aescin molecules. The review concludes on the interaction of β -aescin with phospholipid model membranes in the form of bilayers and Langmuir monolayers. The interaction of β -aescin with lipid bilayers was found to strongly depend on its c m c . At concentrations below the c m c , membrane parameters are modified whereas above the c m c , complete solubilization of the bilayers occurs, depending on lipid phase state and concentration. In the presence of gel-phase phospholipids, discoidal bicelles form; these are tunable in size by composition. The phase behavior of β -aescin with lipid membranes can also be modified by addition of other molecules such as cholesterol or drug molecules. The lipid phase state also determines the penetration rate of β -aescin molecules into lipid monolayers. The strongest interaction was always found in the presence of gel-phase phospholipid molecules.

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“…More recently, unilamellar vesicles with inclusions such as peptides, DNA, cholesterol, etc. have been studied with emphasis on locating the inclusions within the membrane [44][45][46]. The interaction between unilamellar phospholipid vesicles and antimicrobial peptides was probed very quantitatively using SAXS and contrast variation SANS [45].…”

Section: Equilibrium Nanostructure and Interactionsmentioning

confidence: 99%

Synchrotron Scattering Methods for Nanomaterials and Soft Matter Research

Narayanan

Konovalov

2020

Materials

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This article aims to provide an overview of broad range of applications of synchrotron scattering methods in the investigation of nanoscale materials. These scattering techniques allow the elucidation of the structure and dynamics of nanomaterials from sub-nm to micron size scales and down to sub-millisecond time ranges both in bulk and at interfaces. A major advantage of scattering methods is that they provide the ensemble averaged information under in situ and operando conditions. As a result, they are complementary to various imaging techniques which reveal more local information. Scattering methods are particularly suitable for probing buried structures that are difficult to image. Although, many qualitative features can be directly extracted from scattering data, derivation of detailed structural and dynamical information requires quantitative modeling. The fourth-generation synchrotron sources open new possibilities for investigating these complex systems by exploiting the enhanced brightness and coherence properties of X-rays.

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Temperature dependent self-organization of DMPC membranes promoted by intermediate amounts of the saponin aescin

Cited by 26 publications

References 63 publications

Self-Assembly of the Bio-Surfactant Aescin in Solution: A Small-Angle X-ray Scattering and Fluorescence Study

Self-Assembly of the Bio-Surfactant Aescin in Solution: A Small-Angle X-ray Scattering and Fluorescence Study

The Biosurfactant β-Aescin: A Review on the Physico-Chemical Properties and Its Interaction with Lipid Model Membranes and Langmuir Monolayers

Synchrotron Scattering Methods for Nanomaterials and Soft Matter Research

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