Temperature‐dependent resistance to potato virus M in potato (<i>Solanum tuberosum</i>)

Tatarowska, Beata; Plich, Jarosław; Milczarek, Dorota; Flis, Bogdan

doi:10.1111/ppa.13245

Cited by 5 publications

(5 citation statements)

References 19 publications

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“…Possible reasons for this could be: (1) Potato breeders let these varieties grow on the field until autumn where temperatures decrease (personal communication between A. Kellermann and breeders). This gives PVM a higher chance to infect the plant because PVM infection is temperature sensitive, that is, more infections occur at lower temperatures around 20 °C (Tatarowska et al, 2020) (2) Fertilization or metabolism might be different in these varieties changing, for example, cell wall structure which in turn facilitates PVM infection (Kozieł et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

One-step multiplex DiRT-PCR for PLRV, PVY, PVM, PVS, PVA and PVX ready for routine testing directly on tuber sap

2023

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Potato viruses PLRV, PVY, PVM, PVA, PVX and PVS can cause up to 90% loss of potato harvest. Therefore, they are monitored by law in many countries using DAS-ELISA or conventional real-time RT-qPCR. Previously, we developed a multiplex real-time DiRT-PCR (Direct reverse transcript – polymerase chain reaction), which works directly on diluted tuber sap and thus saves time and chemical processing for RNA extraction or time and space in the glasshouse. So far, this method only ran on sap of single tubers which is not practical for routine testing. We are now able to sensitively test for the presence of six viruses in two multiplex reactions using the real-time DiRT-PCR on pooled samples of ten tubers. Here we show that there is an “almost perfect” agreement (Gwet’s AC1 index) comparing this multiplex real-time DiRT-PCR on pooled samples with DAS-ELISA and a commercial RT-qPCR kit with a rapid extraction method. The multiplex real-time DiRT-PCR is now ready to be used for routine testing.

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Section: Discussionmentioning

confidence: 99%

One-step multiplex DiRT-PCR for PLRV, PVY, PVM, PVS, PVA and PVX ready for routine testing directly on tuber sap

2023

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“…The melting curve acquisition program: heating to 95 °C for 15 s; dropping to 60 °C for 30 s, and heating to 95 °C for 15 s. The amplification process was conducted in StepOne™ Plus Real-Time PCR System (Applied Biosystems, Waltham, MA, USA). The expression levels of differential genes were calculated by the 2 −ΔΔCT method ( Tatarowska et al, 2020 ). Data were analyzed using Excel 2022 and Origin 2021.…”

Section: Methodsmentioning

confidence: 99%

Differential gene expression analysis and physiological response characteristics of passion fruit (Passiflora edulis) buds under high-temperature stress

Wang

Zhao

Lai

et al. 2023

PeerJ

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High temperature in summer is an unfavorable factor for passion fruit (Passiflora edulis), which can lead to restricted growth, short flowering period, few flower buds, low fruit setting rate, severe fruit drop, and more deformed fruit. To explore the molecular physiology mechanism of passion fruit responding to high-temperature stress, we use ‘Zhuangxiang Mibao’, a hybrid passion fruit cultivar, as the test material. Several physiological indicators were measured and compared between high-temperature (average temperature 38 °C) and normal temperature (average temperature 25 °C) conditions, including photosynthesis, chlorophyll fluorescence parameters, peroxidase activity (POD), superoxide dismutase activity (SOD) and malondialdehyde content. We performed RNA-seq analysis combined with biochemistry experiment to investigate the gene and molecular pathways that respond to high-temperature stress. The results showed that some physiological indicators in the high-temperature group, including the net photosynthetic rate, stomatal conductance, intercellular CO2 concentration, transpiration rate, and the maximum chemical quantum yield of photosystemII (PSII), were significantly lower than those of the control group. Malondialdehyde content was substantially higher than the control group, while superoxide dismutase and superoxide dismutase activities decreased to different degrees. Transcriptome sequencing analysis showed that 140 genes were up-regulated and 75 genes were down-regulated under high-temperature stress. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation analysis of differentially expressed genes revealed many metabolic pathways related to high-temperature stress. Further investigation revealed that 30 genes might be related to high-temperature stress, such as chlorophyllide a oxygenase (CAO), glutathione (GSH), WRKY transcription factors (WRKY), and heat shock protein (HSP), which have also been reported in other species. The results of real-time fluorescence quantitative PCR and RNA-seq of randomly selected ten genes are consistent, which suggests that the transcriptome sequencing results were reliable. Our study provides a theoretical basis for the mechanism of passion fruit response to high-temperature stress. Also, it gives a theoretical basis for the subsequent breeding of new heat-resistant passion fruit varieties.

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“…The melting curve acquisition program was: heating to 95 °C for 15 s, dropping to 60 °C for 30 s, and heating to 95 °C for 15 s. The amplification process was conducted in StepOne™ Plus Real-Time PCR System (Applied Biosystems). The expression levels of differential genes were calculated by the 2 -△△ CT method (Tatarowska et al,2020).…”

Section: Validation Of Target Gene Using Qrt-pcrmentioning

confidence: 99%

“…The copyright holder for this preprint this version posted September 8, 2022. ; https://doi.org/10.1101/2022.09.07.506887 doi: bioRxiv preprint Plus Real-Time PCR System (Applied Biosystems). The expression levels of differential genes were calculated by the 2 -△△ CT method (Tatarowska et al,2020).…”

Section: Validation Of Target Gene Using Qrt-pcrmentioning

confidence: 99%

Differential gene expression analysis and physiological response characteristics of passion fruit (Passiflora edulis) buds under high-temperature stress

Wang

Zhao²,

Lai³

et al. 2022

Preprint

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High temperature in summer is an unfavorable factor for passion fruit (Passiflora edulis), which can lead to restricted growth, short flowering period, few flower buds, low fruit setting rate, severe fruit drop, and more deformed fruit. To explore the molecular physiology mechanism of passion fruit responding to high-temperature stress, we use Zhuangxiang Mibao, a hybrid passion fruit cultivar, as the test material. Several physiological indicators were measured and compared between high-temperature (average temperature 38℃) and nonmoral temperature (average temperature 25℃) conditions, including photosynthesis, chlorophyll fluorescence parameters, POD, SOD activity and malondialdehyde content. We performed RNA-seq analysis combined with biochemistry experiment to investigate the gene and molecular pathways that respond to high-temperature stress. The results showed that some physiological indicators in the high-temperature group, including the net photosynthetic rate, stomatal conductance, intercellular CO2 concentration, transpiration rate, and the maximum chemical quantum yield of PSⅡ, were significantly lower than those of the control group. Malondialdehyde content was substantially higher than the control group, while superoxide dismutase and superoxide dismutase activities decreased to different degrees. Transcriptome sequencing analysis showed that 140 genes were up-regulated and 75 genes were down-regulated under high-temperature stress. GO and KEGG annotation analysis of differentially expressed genes revealed many metabolic pathways related to high-temperature stress. Further investigation revealed that 30 genes might be related to high-temperature stress, such as CAO, GSH, WRKY, and HSP, which have also been reported in other species. The results of real-time fluorescence quantitative PCR and RNA-seq of randomly selected ten genes are consistent, which suggests that the transcriptome sequencing results were reliable. Our study provides a theoretical basis for the mechanism of passion fruit response to high-temperature stress. Also, it gives a theoretical basis for the subsequent breeding of new heat-resistant passion fruit varieties.

show abstract

Temperature‐dependent resistance to potato virus M in potato (Solanum tuberosum)

Cited by 5 publications

References 19 publications

One-step multiplex DiRT-PCR for PLRV, PVY, PVM, PVS, PVA and PVX ready for routine testing directly on tuber sap

One-step multiplex DiRT-PCR for PLRV, PVY, PVM, PVS, PVA and PVX ready for routine testing directly on tuber sap

Differential gene expression analysis and physiological response characteristics of passion fruit (Passiflora edulis) buds under high-temperature stress

Differential gene expression analysis and physiological response characteristics of passion fruit (Passiflora edulis) buds under high-temperature stress

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