Temperature-dependent photoluminescence of water-soluble quantum dots for a bioprobe

Liu, Tiancai; Huang, Zhen‐Li; Wang, Haiqiao; Wang, Jian-Hao; Li, Xiuqing; Zhao, Yuan‐Di; Luo, Qingming

doi:10.1016/j.aca.2005.11.053

Cited by 90 publications

(70 citation statements)

References 20 publications

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“…39 Different-sized oil-soluble QDs with different emission wavelengths were obtained by controlling the temperature and initial molar ratio of reactants.…”

Section: Preparation Of Cdse-zns Core-shell Qdsmentioning

confidence: 99%

Multifunctional quantum dot–polypeptide hybrid nanogel for targeted imaging and drug delivery

Yang

Yao

et al. 2014

Nanoscale

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A new type of multifunctional quantum dot (QD)-polypeptide hybrid nanogel with targeted imaging and drug delivery properties has been developed by metal-affinity driven self-assembly between artificial polypeptides and CdSe-ZnS core-shell QDs. On the surface of QDs, a tunable sandwich-like microstructure consisting of two hydrophobic layers and one hydrophilic layer between them was verified by capillary electrophoresis, transmission electron microscopy, and dynamic light scattering measurements. Hydrophobic and hydrophilic drugs can be simultaneously loaded in a QD-polypeptide nanogel. In vitro drug release of drug-loaded QD-polypeptide nanogels varies strongly with temperature, pH, and competitors. A drug-loaded QD-polypeptide nanogel with an arginine-glycine-aspartic acid (RGD) motif exhibited efficient receptor-mediated endocytosis in αvβ3 overexpressing HeLa cells but not in the control MCF-7 cells as analyzed by confocal microscopy and flow cytometry. In contrast, non-targeted QD-polypeptide nanogels revealed minimal binding and uptake in HeLa cells. Compared with the original QDs, the QD-polypeptide nanogels showed lower in vitro cytotoxicity for both HeLa cells and NIH 3T3 cells. Furthermore, the cytotoxicity of the targeted QD-polypeptide nanogel was lower for normal NIH 3T3 cells than that for HeLa cancer cells. These results demonstrate that the integration of imaging and drug delivery functions in a single QD-polypeptide nanogel has the potential for application in cancer diagnosis, imaging, and therapy.

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“…39 Different-sized oil-soluble QDs with different emission wavelengths were obtained by controlling the temperature and initial molar ratio of reactants.…”

Section: Preparation Of Cdse-zns Core-shell Qdsmentioning

confidence: 99%

Multifunctional quantum dot–polypeptide hybrid nanogel for targeted imaging and drug delivery

Yang

Yao

et al. 2014

Nanoscale

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“…Irreversible losses are generally caused by chemical processes like ligand loss or oxidative degradation damaging the NPL surface which disturbs the repopulation of mobile holes and electrons. 54 The extent to which the PL intensity is retraced in Fig. 7b is due to reversible losses caused by dynamic and static quenching.…”

Section: −T/τ2mentioning

confidence: 99%

Temperature-dependent optoelectronic properties of quasi-2D colloidal cadmium selenide nanoplatelets

et al. 2017

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Colloidal cadmium selenide (CdSe) nanoplatelets (NPLs) are a recently developed class of efficient luminescent nanomaterials suitable for optoelectronic device applications. A change in temperature greatly affects their electronic bandstructure and luminescence properties. It is important to understand how and why the characteristics of NPLs are influenced, particularly at elevated temperatures, where both reversible and irreversible quenching processes come into the picture. Here we present a study of the effect of elevated temperatures on the characteristics of colloidal CdSe NPLs. We used an effective-mass envelope function theory based 8-band k·p model and density-matrix theory considering exciton-phonon interaction. We observed the photoluminescence (PL) spectra at various temperatures for their photon emission energy, PL linewidth and intensity by considering the exciton-phonon interaction with both acoustic and optical phonons using Bose-Einstein statistical factors. With a rise in temperature we observed a fall in the transition energy (emission redshift), matrix element, Fermi factor and quasi Fermi separation, with a reduction in intraband state gaps and increased interband coupling. Also, there was a fall in the PL intensity, along with spectral broadening due to an intraband scattering effect. The predicted transition energy values and simulated PL spectra at varying temperatures exhibit appreciable consistency with the experimental results. Our findings have important implications for the application of NPLs in optoelectronic devices, such as NPL lasers and LEDs, operating much above room temperature.

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“…In our previous work, the photoluminescence of water-soluble QDs presented sensitive temperature dependence. 16,23 The fluorescence intensity of QDs has irreversible decreased, which is the reason for faint light in well I, Fig. 1͑b͒.…”

Section: Proof Of Conjugation By Sds-pagementioning

confidence: 96%

Special method to prepare quantum dot probes with reduced cytotoxicity and increased optical property

Zhang¹,

Li²,

Wang³

et al. 2010

J. Biomed. Opt.

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Quantum dots (QDs) are widely used in the life sciences because of their novel physicochemical properties. However, the cytotoxity of these nonoparticles have attracted great attention recently because this has not been well resolved. Four probes were synthesized by chemical coupling and protein denaturation with CdSeZnS, CdTe QDs, and transferrin. Sodium dodecyl sulfate polyacrylamide gel electrophoresis and capillary electrophoresis were used to verify the conjugation of these luminescent probes. The cytotoxicity of these four luminescent probes and the original QDs were evaluated in HeLa cells. The results showed that over 92% of HeLa cells were still alive after being exposed to 3.2-microM CdSeZnS QDs capped with denatured transferrin for 72 h. Furthermore, while the probe preparation was very simple, the photoluminescence quantum yield of this probe was 7% higher than the original CdSeZnS QDs. This provides a new way for exploiting QD probes with low cytotoxicity, which will expand applications of nanocomposite assembly in biolabeling and imaging.

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Temperature-dependent photoluminescence of water-soluble quantum dots for a bioprobe

Cited by 90 publications

References 20 publications

Multifunctional quantum dot–polypeptide hybrid nanogel for targeted imaging and drug delivery

Multifunctional quantum dot–polypeptide hybrid nanogel for targeted imaging and drug delivery

Temperature-dependent optoelectronic properties of quasi-2D colloidal cadmium selenide nanoplatelets

Special method to prepare quantum dot probes with reduced cytotoxicity and increased optical property

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