Temperature-dependent Index of Mitotic Interval (τ₀) for Chromosome Manipulation in Korean Rose Bitterling Rhodeus uyekii

Abstract

Eggs of Korean rose bitterling Rhodeus uyekii were collected and fertilized to observe temperature-related cleavage rates and mitotic intervals (τ 0 ). As the water temperature was increased, the slope of first cleavage frequency with elapsed time after fertilization increased, and approximately 30% of fertilized eggs reached first cleavage frequency at every 15 min. At higher temperatures, eggs developed faster and underwent further identical developmental processes. There were strong, negative correlations between τ 0 and water temperatures at all temperatures studied (Y = -1.225X + 70.05, r 2 = 0.988, where Y is τ 0 and X is temperature).

Temperature-dependent Index of Mitotic Interval (τ0) for Chromosome Manipulation in Korean Bullhead, Pseudobagrus fulvidraco

“…Also, the egg yolks are light yellow without oil globules ( Kang & Lee, 1996 ). The radius of Korean bullhead egg was similar to what is found in Kang & Lee, (1996) (1.5±0.06 mm, n =30), but was smaller than that of far eastern catfish ( Silutus asotus ) or Korean rose bitterling ( Rhodeus uyekii ) (3.8±0.04 mm) and bigger than that of black plaice ( Pleuronectes obscurus ) (0.8± 0.01 mm) ( Park & Im, 2010 ; Kim et al, 2011 ).…”

“…In egg development of Korean bullhead on the standard of fertilization time (for example, the initiation time of the first cleavage in fertilized eggs of Korean bullhead and the results obtained from the τ 0 ), it showed the tendency that the initiation time of the first cleavage ran fast according to the water temperature and τ 0 was reduced as well. Except for this study, there were similar results of the reduction of mitotic interval according to rising the water temperature in far eastern catfish, winter flounder ( Pseudopleuronectes americanus ), greenling ( Hexagrammos otakii ), black plaice, Korean rose bitterling ( Park & Im, 2001 ; Park & Johnson, 2002 ; Park et al, 2006 ; Park & Im, 2010 ; Kim et al, 2011 ).…”

“…Until now, the τ 0 has been used to estimate the best time to manipulate chromosomes for the following species: paddlefish ( Polyodon spathula ), shovelnose sturgeon ( Scaphirhynchus platorynchus ), carp ( Cyprinus carpio ), tench ( Tinca tinca ), black crappie ( Pomoxis nigromaculatus ), far eastern catfish ( Silurus asotus ), winter flounder ( Pseudopleuronectes americanus ), greenling ( Hexagrammos otakii ), black plaice ( Pleuronectes obscurus ), and Korean rose bitterling ( Rhodeus uyekii ) ( Flajšhans et al, 1993 ; Shelton & Rothbard, 1993 ; Mims et al, 1997 ; Shelton et al, 1997 ; Gomelsky et al, 2000 ; Park & Im, 2001 ; Park & Johnson, 2002 ; Park et al, 2006 ; Park & Im, 2010 ; Kim et al, 2011 ).…”

Morphometrical Changes on Korean Rose Bitterling, Rhodeus uyekii, in Early Growth Period

“…Early life history of the R. uyekii is as follows: reared in the laboratory, egg and larval development and seasonal sex ratio of Korean endemic fresh water fish R. uyekii , reproductive cycle of the spring-spawning bitterling, osteological study of R. uyekii , elongation of the ovipositor in R. uyekii , temperature-dependent index of mitotic interval (τ 0 ) for chromosome manipulation in R. uyekii and morphometric traits and cytogenetic analysis in induced cross and reciprocal hybrid between R. uyekii and R. notatus ; all of these factors were studied with regard to the R. uyekii (Kim and Han, 1990; Park and Kim, 1990; An, 1995; Kim, 1997; Chae, 2001; Kim et al, 2011, 2012). …”

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“…Various aspects of the Korean rose bitterling have been reported, including: the early life history of laboratory-reared fish; the temperature-dependent (Kim & Han, 1990;Park & Kim, 1990;An, 1995;Kim, 1997;Chae, 2001;Kim, Lim, Gil, & Park, 2011;Kim, Kang, Jang, & Park, 2012). Among the organs of a teleost, the gill, which is comprised of the gill arches, gill filaments and lamella, acts as the essential respiratory organ for life under water and thus develops early in the growth process.…”