Temperature-dependent index of mitotic interval τ0 in greenling Hexagrammos otakii

Abstract

The aim of this study was to establish effective procedures for chromosome manipulation in greenling Hexagrammos otakii Jordan et Starks, which has enormous aquacultural potential. To accomplish this, temperature-dependent measurements of the mitotic intervals (τ 0 ) were carried out. The τ 0 in this fish was determined by averaging the duration of the first and third embryonic divisions at temperatures ranging 5-25°C. At higher temperatures, eggs developed faster and underwent more identical development. For greenling, τ 0 were 341.1 ± 3.60 min at 5°C, 275.5 ± 4.53 min at 10°C, 189.7 ± 6.93 min at 15°C, 99.2 ± 8.27 min at 20°C and 34.2 ± 8.74 min at 25°C. There were strong, negative correlations between the τ 0 and water temperatures at all temperatures studied (Y = −79.3X + 425.3, R 2 = 0.9968, where Y is the mitotic interval and X is the temperature).

Egg Development and Mitotic Interval (τ0) in Black Plaice, Pleuronectes obscurus (Herzenstein)

“…Temperaturedependence of τ 0 in black plaice is similar to observations for winter flounder (Pseudopleuronectes americanus; Park and Johnson, 2002), far eastern catfish (Silurus asotus; Park and Im, 2001), greenlings (Hexagrammos otakii; Park et al, 2006), baltic herring (Clupea harengus membras; Saat and Veersalu, 1996b), perch (Perca fluviatilis; Saat and Veersalu, 1996a), and ruffe (Gymnocephalus cernuus; Saat and Veersalu, 1996a). Additionally, the relationships between τ 0 and water temperature in fish are typically curvilinear, providing that temperatures are within the range for which the species of fish naturally spawns and develops Veersalu, 1996a, 1996b;Park and Im, 2001;Park and Johnson, 2002;Park et al, 2006). Observed linear relationship between τ 0 and temperature was consistent with data for the developmental rates of black carp and winter flounder (Shelton and Rothbard, 1993;Park and Johnson, 2002).…”

“…Fish were weighed to the nearest 0.01 g using an electronic balance (JW-1; Acom, Pocheon, Korea), and body lengths were measured to the nearest 0.01 cm using digital vernier calipers (CD-20CP; Mitutoyo, Kawasaki, Japan). Sperm and eggs were artificially fertilized using the dry process (Park et al, 2006).…”

Effect of temperature on incubation period and hatching success of fat greenling (Hexagrammos otakiiJordan & Starks) eggs

“…To date, several reports are available on reproduction (Wen, Wang, Mou, Chen, Yao & Chen ; Pan, Hu, Gao, Jian, Zhang, Wang & Guo ), development (Park, Kim, Woo, Oh, Kim & Hur ; Lee & Cho ), feeding physiology (Kim & Kang ; Kwak, Baecka & Klumppb ), disease (Ki, Kyoung & Chun ; Park, Jo, Lee, Kim, Park, Hur, Yoo & Song ), nutritional composition (Masahiro, Yasuyuki & Atsunobu ) and polymorphic microsatellites (Chen, Miao, Shao, Tian & Liao ) of H. otakii . However, very little is known about the biological and ecological requirements of this species as a basis for fishery management, commercial cultivation and replenishment of natural populations.…”

Changes of hematological characteristics of greenling (Hexagrammos otakii Jordan and Starks) by anaesthetics

“…The greenling is a number of the typical seawater Hexagrammidae and a commercially important greenling species, and this species is a favorite seawater food fish because of its taste and tender meat [7]. Previous study reported the anesthesia effects of MS-222 and lidocaine-HCl on the greenling [3].…”

“…The greenling, Hexagrammos otakii (Jordan and Starks) is commonly found in coastal areas of Korea throughout the year [7]. The greenling is a number of the typical seawater Hexagrammidae and a commercially important greenling species, and this species is a favorite seawater food fish because of its taste and tender meat [7].…”