Temperature-dependent growth and sexuality of the ciguatoxin producer dinoflagellate Gambierdiscus spp. in cultures established from the Canary Islands

Ramilo, Isabel; Figueroa, Rosa Isabel; Rayón-Viña, Fernando; Cuadrado, Ángeles; Bravo, Isabel

doi:10.1016/j.hal.2021.102130

Cited by 9 publications

(5 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regarding C-CTXs, the studies performed until now have shown that the most commonly found analogues are the Caribbean Ciguatoxin-1 and -2, named as C-CTX1 and C-CTX2 (Estevez et al 2019b). Ciguatoxin-producing dinoflagellates were initially found in Indo-pacific oceans and in the Caribbean Sea reviewed by Shmukler and Nikishin (Shmukler and Nikishin 2017); however, during the last decades, as a possible result of climate change (Ramilo et al 2021;Xu et al 2016) and anthropogenic activities, including the globalization of trade, these microalgal organisms have spread to European coasts (Botana 2016;FAO 2020;Otero et al 2010), and this fact rises the need to fully revaluate the potency of Caribbean and Pacific ciguatoxin analogues.…”

Section: Introductionmentioning

confidence: 99%

In Silico Simulations and Functional Cell Studies Evidence Similar Potency and Distinct Binding of Pacific and Caribbean Ciguatoxins

et al. 2022

View full text Add to dashboard Cite

Ciguatoxins (CTX) cause ciguatera poisoning, which is the most common reported human food poisoning related to natural marine toxins. Pacific ciguatoxins are the most abundant and studied CTX analogues; however, the growing distribution of Caribbean analogues and the limited data available on their biological effects make necessary to re-evaluate their relative potency. For decades, the guidelines established by regulatory agencies have assumed that the potency of the Caribbean CTXs were tenfold lower than the Pacific CTXs. We present here an integrated study involving Neuro-2a cells (the method used worldwide to test ciguatoxins), electrophysiological assays, and in silico simulations that evidence the similar cytotoxicity of Caribbean and Pacific ciguatoxins and their asymmetry binding within sodium channels. The binding mode of the toxins was first explored by molecular docking using the GOLD program and the resulting binary complexes were further studied by Molecular Dynamics simulation studies using the molecular mechanics force field AMBER. The simulation studies explain their distinct impact on the activation potential of the channel as experimentally observed and provide a detailed picture of the effects caused by these toxins on an atomic scale. Graphical Abstract

show abstract

Section: Introductionmentioning

confidence: 99%

In Silico Simulations and Functional Cell Studies Evidence Similar Potency and Distinct Binding of Pacific and Caribbean Ciguatoxins

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Regarding C-CTXs, the studies performed until now have shown that the most commonly found analogues are the Caribbean Ciguatoxin-1 and − 2, named as C-CTX1 and C-CTX2 (Estevez et al 2019b). Ciguatoxin-producing dino agellates were initially found in Indo-paci c oceans and in the Caribbean Sea (Shmukler and Nikishin 2017), however, during the last decades, as a possible result of climate change (Ramilo et al 2021; Xu et al 2016) and anthropogenic activities including the globalization of trade, these microalgal organisms have spread to the European coasts (Botana 2016;FAO 2018;Otero et al 2010).…”

Section: Introductionmentioning

confidence: 99%

In silico simulations and functional cell studies evidence similar potency and distinct binding of Pacific and Caribbean ciguatoxins

Raposo-García

Castro

Lence

et al. 2022

Preprint

View full text Add to dashboard Cite

Ciguatoxins (CTX) cause ciguatera fish poisoning, which is the most common reported hu-man food poisoning related to natural marine toxins. Pacific ciguatoxins are the most abundant and studied CTX analogues, however, the growing distribution of Caribbean analogues and the limited data available on their biological effects makes necessary to re-evaluate their relative potency. For decades, the guidelines established by regulatory agencies have assumed that the potency of the Caribbean CTXs were 10-fold lower than the Pacific CTXs. We present here an integrated study involving Neuro-2a MTT and electrophysiological assays and in silico simulations that evidence the similar cytotoxicity of both toxins and the asymmetry binding of CTXs with sodium channels. The simulation studies explain their distinct impact on the activation potential of the channel as experimentally observed and provided a detailed picture of the effects caused by these toxins on an atomic scale.

show abstract

“…in the Canary Islands can tolerate low winter conditions and survive until the summer. Ramilo et al [53] also report other studies that show that G. australes can tolerate temperatures as low as 13 • C. The CPR is towed behind ships at a depth of 4-10 m. Surface water temperatures along the cruise track for 2014 ranged from 15 to 17 • C. Thus, there is a potential for Gambierdiscus to survive in coastal waters, depending on its substrate, along the Iberian coast until warmer summer sea temperatures (e.g., climate change) could initiate growth to cause a toxic event. However, eDNA in marine environments decays exponentially with a half-life of 26 h, and it is more persistent offshore [54], so it is possible Gambierdiscus may not have been alive.…”

Section: Discussionmentioning

confidence: 99%

“…Growth experiments performed on multiple strains of five Gambierdiscus species by Ramilo et al [53] showed that G. excentricus and G. silvae were better adapted to lower temperatures but could not survive below 17 • C, whereas G. australes survived at temperatures as low as 15 • C and exhibited the broadest range of temperature tolerances, hence its global distribution. All species that tolerated lower temperatures were kept at low temperatures for up to six months, and when returned to 25 • C, they started to grow.…”

Section: Discussionmentioning

confidence: 99%

Mapping Selected Emergent Marine Toxin-Producing Organisms Using Historical Samples with Two Methods (Biosensors and Real-Time PCR): A Comparison of Resolution

Mengs,

Stern,

Clarke

et al. 2024

Applied Microbiology

View full text Add to dashboard Cite

The Continuous Plankton Recorder (CPR) survey is a valuable resource for mapping changes in plankton distribution and understanding harmful algal ecology because of its breadth and longevity. Preservation methods with formalin degrade DNA, making it difficult to use as a molecular tool for archived marine samples. DNA was extracted from CPR samples immediately after collection, seven months later and after nine years of storage from a cruise track along the Iberian Peninsula. PCR reactions performed from the nine-year timepoint were hybridized to probes in an electrochemical biosensor and compared to results obtained from RT-PCR performed at two earlier time points. The successful identification of Pseudo-nitzschia spp., Prorocentrum lima, Alexandrium minutum, Alexandrium ostenfeldii, Gambierdiscus spp. and Coolia spp. was documented. The biosensor analysis outperformed RT-PCR, allowing us to document certain tropical toxic dinoflagellates, viz., Gambierdiscus and Coolia, that produce human ciguatoxins and Coolia toxins, respectively. These non-native algal toxins can accumulate, pervade the food web and negatively impact human food security. This supports the northerly movement of microalgae with climate change in offshore Iberian peninsular waters. This study highlights biosensors as a cost-effective tool for the offshore monitoring of HAB species and advances molecular technologies for long-term CPR datasets that have limited records of harmful algae. DNA from formalin-preserved CPR samples is degraded, so the use of a short, multiprobe biosensor can augment historical plankton records with contemporary methods that also capture infrequently occurring benthic taxa carried in surface waters. The integration of probe-based biosensor technologies offers a promising avenue for exploring plankton dynamics in response to environmental changes.

show abstract

Temperature-dependent growth and sexuality of the ciguatoxin producer dinoflagellate Gambierdiscus spp. in cultures established from the Canary Islands

Cited by 9 publications

References 55 publications

In Silico Simulations and Functional Cell Studies Evidence Similar Potency and Distinct Binding of Pacific and Caribbean Ciguatoxins

In Silico Simulations and Functional Cell Studies Evidence Similar Potency and Distinct Binding of Pacific and Caribbean Ciguatoxins

In silico simulations and functional cell studies evidence similar potency and distinct binding of Pacific and Caribbean ciguatoxins

Mapping Selected Emergent Marine Toxin-Producing Organisms Using Historical Samples with Two Methods (Biosensors and Real-Time PCR): A Comparison of Resolution

Contact Info

Product

Resources

About