Temperature-Dependent Changes in the Sugar and Fatty Acid Composition of Lipopolysaccharides from Yersinia enterocolitica Strains

Wartenberg, Klaus; Knapp, W.; Ahamed, N.M.; Widemann, C.; Mayer, Hubert

doi:10.1016/s0174-3031(83)80103-6

Cited by 13 publications

(10 citation statements)

References 21 publications

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“…The LPS of Y. enterocolitica 0:3 has been studied chemically and recently also genetically. The O-antigen of Y. enterocolitica serotype 0:3 is a homopolymer composed of 6-deoxy-L-altrose repeating units, linked together by 1,2 linkages (Hoffman et aL, 1980;Wartenberg et aL, 1983). Recently, the chemical structure of the inner core polysaccharide composed of seven sugar residues was determined (Radziejewska-Lebrecht et aL, 1994) and preliminary structural analysis showed that there are six sugar residues in the outer core (J. Radziejewska-Lebrecht, M. Skurnik, A. S. Shashkov, and H. Mayer, unpublished).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, the chemical structure of the inner core polysaccharide composed of seven sugar residues was determined (Radziejewska-Lebrecht et aL, 1994) and preliminary structural analysis showed that there are six sugar residues in the outer core (J. Radziejewska-Lebrecht, M. Skurnik, A. S. Shashkov, and H. Mayer, unpublished). The expression of the hornopolymeric O-antigen is temperature regulated such that the number of repeating units per LPS molecule is higher when bacteria are grown at 22°C (Acker et aL, 1981;AI-Hendy et al, 1991b;Kawaoka et al, 1983;Wartenberg et aL, 1983). Acker and co-workers (Acker et al, 1981) demonstrated that the enterobacterial common antigen (ECA) of Y. enterocolitica serotype 0:3 strain was sterically blocked by abundant O-antigen from ECA-specific antibody molecules in bacteria grown at 22°C but not in bacteria grown at 37°C.…”

Section: Discussionmentioning

confidence: 99%

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A novel locus of Yersinia enterocolitica serotype O:3 involved in lipopolysaccharide outer core biosynthesis

Skurnik

Venho

Toivanen

et al. 1995

Molecular Microbiology

126

169

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Yersinia enterocolitica serotype O:3 strain 6471/76-c (YeO3-c) was sensitive to bacteriophage phi R1-37 when grown at 37 degrees C but not when grown at 22 degrees C because of steric hindrance by abundant lipopolysaccharide (LPS) O-side chain (O-antigen) expressed at 22 degrees C. The transposon library of YeO3-c was grown at 37 degrees C and screened for phage phi R1-37-resistant transposon insertion mutants. Three types of mutant were isolated: (i) phage receptor mutants expressing O-antigen (LPS-smooth), (ii) phage receptor mutants not expressing O-antigen (LPS-rough), and (iii) LPS-smooth mutants with the phage receptor constitutively sterically blocked. Mutant type (i) was characterized in detail; the transposon insertion inactivates an operon, named the trs operon. The main findings based on this mutant are: (i) the frs operon is involved in the biosynthesis of the LPS outer core in YeO3-c; the nucleotide sequence of the trs operon revealed eight novel genes showing similarly to known polysaccharide biosynthetic genes of various Gram-negative bacteria as well as to capsule biosynthesis genes of Staphylococcus aureus; (ii) the biosynthesis of the core of YeO3-c involves at least two genetic loci; (iii) the trs operon is required for the biosynthesis of the bacteriophage phi R1-37 receptor structures; (iv) the homopolymeric O-antigen of YeO3-c is ligated to the inner core in Y. enterocolitica O:3; (v) the trs operon is located between the adk-hemH and galE-gsk gene pairs in the Y. enterocolitica chromosome; and (vi) the phage phi R1-37 receptor is present in many but not in all Y. enterocolitica serotypes. The results also allow us to speculate that the trs operon is a relic of the ancestral rfb region of Y. enterocolitica O:3 carrying genes indispensable for the completion of the core polysaccharide biosynthesis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A novel locus of Yersinia enterocolitica serotype O:3 involved in lipopolysaccharide outer core biosynthesis

Skurnik

Venho

Toivanen

et al. 1995

Molecular Microbiology

126

169

View full text Add to dashboard Cite

show abstract

“…In this context, the protective impact of surface-associated growth (observed in Y. enterolitica as well as Exiguobacterium spp., and for at least one straintemperature combination in L. monocytogenes [Vishnivetskaya et al, 2007;Azizoglu et al, 2009]) may also reflect the increasingly recognized impact of planktonic versus surface growth on the physiology of microorganisms. In the case of Y. enterocolitica, surface antigenic changes in response to growth temperature have been noted (Kawaoka et al, 1982;Wartenberg et al, 1983), but the dependence of such changes on planktonic versus surface growth is not known. Further studies will be needed to assess the involvement of these and other mechanisms involved in low-temperature adaptations Venkitanarayanan, 2005, 2009;Goverde et al, 1998;Neuhaus et al, 2003;Palonen et al, 2010) on cryotolerance of these microorganisms.…”

Section: Discussionmentioning

confidence: 99%

Impact of Growth Temperature and Agar Versus Liquid Media on Freeze–Thaw Tolerance ofYersinia enterocolitica

Azizoglu

Kathariou²

2010

Foodborne Pathogens and Disease

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Yersinia enterocolitica is a foodborne pathogen well known for its ability to grow at low temperatures. Recent studies with another psychrotrophic foodborne pathogen, Listeria monocytogenes, revealed that temperature of growth had pronounced impact on survival following repeated freezing and thawing (cryotolerance). Listerial cryotolerance was significantly more pronounced when bacteria were grown at 37 degrees C than following growth at either 4 degrees C or 25 degrees C. However, it is not known whether such impact of growth temperature is a general adaptation shared with other foodborne pathogens. In this study, we investigated the impact of growth temperature (4 degrees C, 25 degrees C, and 37 degrees C) on cryotolerance of Y. enterocolitica. In strong contrast to findings previously obtained with Listeria spp., cryotolerance of Y. enterocolitica was impaired following growth in liquid media at 37 degrees C, with cell concentration dropping to undetectable levels (<10(1) colony forming unit/mL) following as few as six freeze-thaw cycles. On the other hand, when the bacteria were grown at 4 degrees C, cryotolerance was significantly higher (p < 0.05), and substantial survival was maintained even after 18 cycles (2-5 log reduction, depending on strain). Enhanced cryotolerance was also observed with cultures grown at 25 degrees C. Bacteria grown at 37 degrees C on agar were significantly more cryotolerant than following growth at 37 degrees C in liquid media (p < 0.05). The data suggest species-specific impact of growth temperature and liquid versus agar medium on cryotolerance of cold-tolerant bacteria.

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“…At room temperature, wild-type YeO3 strains exhibit more abundant O-PS than at 37°C (Acker et al 1981;al-Hendy et al 1991;Kawaoka et al 1983;Wartenberg et al 1983).…”

Section: Lps: the Multifaceted Surface Componentmentioning

confidence: 99%

Bacterial Cell Surface Structures in Yersinia enterocolitica

Białas

Kasperkiewicz

Radziejewska‐Lebrecht

et al. 2012

Arch. Immunol. Ther. Exp.

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Temperature-Dependent Changes in the Sugar and Fatty Acid Composition of Lipopolysaccharides from Yersinia enterocolitica Strains

Cited by 13 publications

References 21 publications

A novel locus of Yersinia enterocolitica serotype O:3 involved in lipopolysaccharide outer core biosynthesis

A novel locus of Yersinia enterocolitica serotype O:3 involved in lipopolysaccharide outer core biosynthesis

Impact of Growth Temperature and Agar Versus Liquid Media on Freeze–Thaw Tolerance ofYersinia enterocolitica

Bacterial Cell Surface Structures in Yersinia enterocolitica

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