Temperature dependence of membrane sealing following transection in mammalian spinal cord axons

Shi, Riyi; Pryor, John H.

doi:10.1016/s0306-4522(00)00096-8

Cited by 48 publications

(46 citation statements)

References 56 publications

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“…This is also in agreement with our previous histology and electrophysiology studies. 15,17,19,22 Using the double sucrose gap recording device 15 ± 17,19 and an HRPexclusion assay, 17,18,23 we have demonstrated that spinal cord strips extracted by the same procedure used in this study maintained cellular membrane integrity, as well as normal electrophysiological functions. We believe this is due to the combination of cold Kreb's solution perfusion, fast surgery procedure, and subsequent su cient perfusion of Kreb's at physiological temperature.…”

Section: Discussionmentioning

confidence: 63%

“…We believe this is due to the combination of cold Kreb's solution perfusion, fast surgery procedure, and subsequent su cient perfusion of Kreb's at physiological temperature. 17,18 In summary, in this current in vitro model, the isolation surgery produced minimal damage, which makes it possible for the tissue to show the increase of ROS caused by deliberate compression injury and be sensitive to treatment.…”

Section: Discussionmentioning

confidence: 78%

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The increase of reactive oxygen species and their inhibition in an isolated guinea pig spinal cord compression model

Luo

Robinson

et al. 2002

Spinal Cord

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Study design:In vitro studies using isolated guinea pig spinal cord. Objectives: To develop an alternative model using isolated guinea pig spinal cord, which can be used to screen antioxidants for in vivo SCI treatment. Setting: Department of Basic Medical Sciences, Purdue University, West Lafayette, Indiana, USA. Methods: The compression injury was induced by a constant-displacement of 5-s compression of spinal cord using a modi®ed forceps possessing a spacer. Reactive oxygen species (ROS) were evaluated using three distinct methods:¯uorescence microscopy, lipid peroxidation assay, and¯ow cytometry. Results: The injury-mediated ROS increases are comparable with other in vivo studies and consistent with our previous observation using a similar injury model and measured with electrophysiological and anatomical technique. Further, ascorbic acid, hypothermia, or the combination of both signi®cantly suppressed superoxide and lipid peroxidation. The combination treatment was the most e ective when compared with ascorbic acid or hypothermia alone. Conclusion: This in vitro model has the advantage of replicating some of the in vivo conditions while gaining the ability to control the experimental conditions. This in vitro model is suitable to study the mechanisms of ROS generation and degradation and can also be used to critically evaluate the e ective suppressor of ROS in the contents of spinal cord traumatic injury.

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Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 78%

The increase of reactive oxygen species and their inhibition in an isolated guinea pig spinal cord compression model

Luo

Robinson

et al. 2002

Spinal Cord

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“…Rice seedlings were grown on MS agar medium under continuous light and 14-day-old seedlings were treated with NaCl, abscisic acid, and low temperature as described previously (Shi et al 2000). Determination of gene expression in roots and shoots was performed as described previously (Shi et al 2000).…”

Section: Stress Treatment and Rt-pcr Analysismentioning

confidence: 99%

A transcription factor "OsNAC075" is essential for salt resistance in rice (Oryza sativa L.)

Jung¹,

Lee²,

Kyoo³

2011

Journal of Plant Biotechnology

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Salt stress is a major environmental factor influencing plant growth and development. To identify salt tolerance determinants, we systematically screened salt sensitive rice mutants by use of the Activator/Dissociation (Ac/Ds) transposon tagging system. In this study, we focused on the salt sensitive mutant line, designated SSM-1. A gene encoding a NAC transcription factor homologue was disrupted by the insertion of a Ds transposon into SSM-1 line. The OsNAC075 gene (EU541472) has 7 exons and encodes a protein (486-aa) containing the NAC domain in its N-terminal region. Sequence comparison showed that the OsNAC075 protein had a strikingly conserved region at the N-terminus, which is considered as the characteristic of the NAC protein family. OsNAC075 protein was orthologous to Arabidopsis thaliana ANAC075. Phylogenetic analysis confirmed OsNAC075 belonged to the OsNAC3 subfamily, which plays an important role in response to stress stimuli. RT-PCR analysis showed that the expression of OsNAC075 gene was rapidly and strongly induced by stresses such as NaCl, ABA and low temperature (4℃). Our data suggest that OsNAC075 holds promising utility in improving salt tolerance in rice.

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“…The effects of mechanical disruption on neuronal membranes have been extensively studied both in vitro and in vivo [4,11,[13][14][15][16][17][18][19][20][21][22][23][24] . A strong correlation between membrane resealing and functional recovery, as well as neuronal survival, has long been established [4,13,17,18] .…”

mentioning

confidence: 99%

“…A strong correlation between membrane resealing and functional recovery, as well as neuronal survival, has long been established [4,13,17,18] . it has been shown that enhancing membrane repair in rodent spinal cord axons using various physical, chemical, and biochemical interventions is associated with significant functional recovery [4,18,19,[25][26][27] . Membrane resealing is required for both the long-term survival of the cell as well as the prevention of further degeneration in severed axons or those crushed axons progressing to secondary axotomy.…”

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confidence: 99%

Polyethylene glycol repairs membrane damage and enhances functional recovery: a tissue engineering approach to spinal cord injury

Shi

2013

Neurosci. Bull.

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Temperature dependence of membrane sealing following transection in mammalian spinal cord axons

Cited by 48 publications

References 56 publications

The increase of reactive oxygen species and their inhibition in an isolated guinea pig spinal cord compression model

The increase of reactive oxygen species and their inhibition in an isolated guinea pig spinal cord compression model

A transcription factor "OsNAC075" is essential for salt resistance in rice (Oryza sativa L.)

Polyethylene glycol repairs membrane damage and enhances functional recovery: a tissue engineering approach to spinal cord injury

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