Temperature dependence of anaerobic TCE-dechlorination in a highly enriched Dehalococcoides-containing culture

Friis, Anne Kirketerp; Heimann, Axel Colin; Jakobsen, Rasmus; Albrechtsen, Hans‐Jørgen; Cox, Evan; Bjerg, Poul Løgstrup

doi:10.1016/j.watres.2006.09.026

Cited by 70 publications

(49 citation statements)

References 32 publications

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“…This temperature was chosen because it was greater than the expected optimum of approximately 30°C (Friis et al 2007b). TCE and lactate were added initially and the microcosms were bioaugmented with KB-1 TM at various temperatures while cooling (Fig.…”

Section: Experimental Approachmentioning

confidence: 99%

“…1). This was carried out in order to investigate whether the previously determined temperature optima in diluted cultures (Friis et al 2007b) can be effectively applied in aquifer microcosms collected after a thermal treatment.…”

Section: Experimental Approachmentioning

confidence: 99%

“…A zero order kinetic model (Friis et al 2007b) and a competitive Monod kinetic model (Cupples et al 2004;Yu et al 2005) were applied. Both models assumed that presence of electron donor is not limiting for dechlorination.…”

Section: Dechlorination Growth Ratesmentioning

confidence: 99%

“…This suggests a potential for biological remediation after thermal treatment as temperatures remain elevated, because rates for either partial or complete dechlorination of TCE to ethene are increased at temperatures of approximately 30°C for isolates (He et al 2003) and between 15°C and 30°C for mixed cultures (Friis et al 2007b).…”

mentioning

confidence: 99%

“…In addition, the temperature optima of dechlorination were determined in the diluted mixed culture KB-1 TM (Friis et al 2007b). In the current study the same culture was used, but here we intended to mimic field conditions.…”

mentioning

confidence: 99%

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Microcosm evaluation of bioaugmentation after field-scale thermal treatment of a TCE-contaminated aquifer

et al. 2007

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This paper investigates effects of combining thermal and biological remediation, based on laboratory studies of trichloroethene (TCE) degradation. Aquifer material was collected 6 months after terminating a full-scale Electrical Resistance Heating (ERH), when the site had cooled from approximately 100 degrees C to 40 degrees C. The aquifer material was used to construct bioaugmented microcosms amended with the mixed anaerobic dechlorinating culture, KB-1(TM), and an electron donor (5 mM lactate). Microcosms were bioaugmented during cooling at 40, 30, 20, and 10 degrees C, as temperatures continually decreased during laboratory incubation. Redox conditions were generally methanogenic, and electron donors were present to support dechlorination. For microcosms bioaugmented at 10 degrees C and 20 degrees C, dechlorination stalled at cis-dichloroethene (cDCE) and vinyl chloride (VC) 150 days after bioaugmentation. However, within 300 days of incubation ethene was produced in the majority of these microcosms. In contrast, dechlorination was rapid and complete in microcosms bioaugmented at 30 degrees C. Microcosms bioaugmented at 40 degrees C also showed rapid dechlorination, but stalled at cDCE with partial VC and ethene production, even after 150 days of incubation when the temperature had decreased to 10 degrees C. These results suggest that sequential bioremediation of TCE is possible in field-scale thermal treatments after donor addition and bioaugmentation and that the optimal bioaugmentation temperature is approximately 30 degrees C. When biological and thermal remediations are to be applied at the same location, three bioremediation approaches could be considered: (a) treating TCE in perimeter areas outside the source zone at temperatures of approximately 30 degrees C; (b) polishing TCE concentrations in the original source zone during cooling from approximately 30 degrees C to ambient groundwater temperatures; and (c) using bioremediation in downgradient areas taking advantages of the higher temperature and potential release of organic matter.

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Section: Experimental Approachmentioning

confidence: 99%

Section: Experimental Approachmentioning

confidence: 99%

Section: Dechlorination Growth Ratesmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Microcosm evaluation of bioaugmentation after field-scale thermal treatment of a TCE-contaminated aquifer

et al. 2007

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Review of reactive kinetic models describing reductive dechlorination of chlorinated ethenes in soil and groundwater

Chambon¹,

Bjerg²,

Scheutz³

et al. 2012

Biotech & Bioengineering

107

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Reductive dechlorination is a major degradation pathway of chlorinated ethenes in anaerobic subsurface environments, and reactive kinetic models describing the degradation process are needed in fate and transport models of these contaminants. However, reductive dechlorination is a complex biological process, where many microbial populations including dechlorinating, fermentative, methanogenic, iron and sulfate reducing, interact. In this article the modeling approaches and the experimental data needed to calibrate them are reviewed, classified, and discussed. Model approaches considered include first order kinetics, Monod kinetics to describe sequential reductive dechlorination and bacterial growth, and metabolic models which simulate fermentation and redox processes interacting with reductive dechlorination processes. The review shows that the estimated kinetic parameters reported vary over a wide range, and that experimental microbial data are scarce. Very few studies have been performed evaluating the influence of sulfate and iron reduction, and contradictory conclusions on the interaction of redox processes with reductive dechlorination have been reported. The modeling approaches for metabolic reductive dechlorination employing different descriptions of the interaction between redox and dechlorination processes and competition for hydrogen are classified. The current concepts lead to different results, suggesting a need for further investigations on the interactions between the microbial communities performing dechlorination and redox processes, including the establishment of biomarkers quantifying dechlorination, and on geochemical characterization. Finally, the relevance of laboratory data and the development of practical modeling tools for field applications are discussed.

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Source Remediation Challenges

Abriola

Christ

Pennell

et al. 2012

SERDP/ESTCP Environmental Remediation Technology

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Temperature dependence of anaerobic TCE-dechlorination in a highly enriched Dehalococcoides-containing culture

Cited by 70 publications

References 32 publications

Microcosm evaluation of bioaugmentation after field-scale thermal treatment of a TCE-contaminated aquifer

Microcosm evaluation of bioaugmentation after field-scale thermal treatment of a TCE-contaminated aquifer

Review of reactive kinetic models describing reductive dechlorination of chlorinated ethenes in soil and groundwater

Source Remediation Challenges

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