1975
DOI: 10.1016/0006-291x(75)90548-3
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Temperature dependence for physical and functional reconstitution of 30S ribosomes of E. coli

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1976
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Cited by 7 publications
(5 citation statements)
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“…Instead of having a relative sedimentation velocity of 30S, the particle containing the fragment sediments at approximately 28S (Fig 7), an assembly intermediate which has been previously observed (31,32).…”
Section: Discussionmentioning
confidence: 51%
“…Instead of having a relative sedimentation velocity of 30S, the particle containing the fragment sediments at approximately 28S (Fig 7), an assembly intermediate which has been previously observed (31,32).…”
Section: Discussionmentioning
confidence: 51%
“…Therefore, we conclude that DnaK is not required for biosynthesis of ribosomal subunits, at least at 30°C, nor for the capabilities of ribosomal components to reconstitute active ribosomal subunits. Wireman and Sypherd (1975) detected disparity between the physical formation of 30S particles and the acquisition of activity when the reconstitution is performed with mature 16S rRNA: formation of 30S particles occurs at temperatures 15°C lower than the appearance of active subunits. Both features were reported to be improved by the DnaK system (Maki et al 2002), that is, reconstituted particles became more homogeneous as shown with sucrosegradient profiles, and tRNA binding occurred in the absence of a heat-activation step.…”
Section: Dnak Chaperones and Ribosome Assemblymentioning
confidence: 95%
“…Inactivation corresponds to conformational changes rather than to the loss of ribosomal components (Zamir et al, 1974). As a matter of fact, ab initio reconstitution of the 3OS subunit from isolated rRNA and ribosomal proteins exhibits too high energy requirements (Wireman and Sypherd, 1975) and would hardly explain the Mg2+ dependence of the rate-limiting process we measured (Figure 4b). No conformational change of the 50S subunit appears from the association kinetics we performed in the absence of the ammonium ion (Table II).…”
Section: Discussionmentioning
confidence: 78%