Temperature Conversion Factors, Activation Energy,
Relative Substrate Specificity and Optimum pH of
Adenosine Deaminase from Human Serum and Tissues

Giusti, G; Gakis, C

doi:10.1159/000459567

Cited by 123 publications

(49 citation statements)

References 12 publications

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“…Determination of pleural fluid 2'-deoxyadenosine deaminase activity, though a necessary step in the estimation of ADA-2 activity [15], does not seem to be of clinical utility per se, even though its diagnostic efficiency in this study was as high as that of total ADA activity and almost as high as that of ADA-2 activity. The diagnostic efficiency of the 2'-deoxyadenosine deaminase/ADA activity ratio was poor: although this ratio was <0.49 in all the tuberculous effusions (sensitivity 100%), one parapneumonic and eight neoplastic effusions also had 2'-deoxyadenosine deaminase/ADA activity ratios below this threshold (specificity 64%).…”

Section: Discussionmentioning

confidence: 58%

“…Briefly, duplicate 25 µL samples were incubated for 60 min at 37°C with 500 µL of 21 mM adenosine (one sample) or 21 mM 2'-deoxyadenosine (the other) in 50 mM phosphate buffer [15], and the ammonium ion released was determined by reaction for 30 min with 1.5 mL of phenol nitroprusside (106 mM phenol plus 0.17 mM sodium nitroprusside) in the presence of 1.5 mL of sodium hypochlorite (11 mM NaOCl plus 125 mM NaOH), absorption at 628 nm then being read in a spectrophotometer. To control for ammonium present before addition of exogenous adenosine, untreated samples were run in parallel.…”

Section: Methodsmentioning

confidence: 99%

“…To control for ammonium present before addition of exogenous adenosine, untreated samples were run in parallel. Estimated ADA-2 activity was calculated from the ADA and 2'-deoxyadenosine deaminase activities using the affinity of ADA-2 for the two substrates [15]. The results were expressed in U·L -1 .…”

Section: Methodsmentioning

confidence: 99%

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Adenosine deaminase (ADA) isoenzyme analysis in pleural effusions: diagnostic role, and relevance to the origin of increased ADA in tuberculous pleurisy

Valdés¹,

José²,

Álvarez³

et al. 1996

Eur Respir J

143

100

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The rise in adenosine deaminase (ADA) activity in the pleural fluid of tuberculous pleurisy patients, though used for diagnosis, is of unknown origin. In this work, we determined ADA activity and the activities of 2'-deoxyadenosine deaminase and ADA-2 in 350 patients. We also considered whether the results throw light on the origin of high pleural fluid ADA in tuberculous pleurisy and estimated the diagnostic efficiency of 2'-deoxyadenosine deaminase, ADA-2 and total ADA activities with and without the inclusion of the 2'-deoxyadenosine deaminase/ADA activity ratio in a combined criterion.The 350 pleural effusions were classified by previously established criteria as transudates (60 males/18 females) or as tuberculous (49 males/27 females), neoplastic (50 males/39 females), parapneumonic (36 males/19 females), empyematous (11 males/ 3 females), or miscellaneous (25 males/13 females) exudates. Total ADA, ADA-2 and 2'-deoxyadenosine deaminase activities were, respectively, 127.5±2.9, 103±29.5 and 42.8±14 U·L -1 in tuberculous exudates. With diagnostic thresholds of 47, 40 and 22 U·L -1 respectively, the sensitivities of ADA, ADA-2 and 2'-deoxyadenosine deaminase for tuberculosis were 100, 100 and 95%; their specificities 91, 96 and 92%; and their efficiencies 93, 97 and 93%, respectively. One hundred and one effusions (all 76 tuberculous, 12 neoplastic, 4 parapneumonic and 9 empyematous exudates) had total ADA levels >47 U·L -1 ; of these, 8 neoplastic, 1 parapneumonic and all the tuberculous exudates had a 2'-deoxyadenosine deaminase/ADA activity ratio <0.49. The criterion of simultaneously having ADA >47 U·L -1 , ADA-2 >40 U·L -1 and a 2'-deoxyadenosine deaminase/ADA activity ratio <0.49 was satisfied by all the tuberculous effusions but only eight others (all neoplastic) (sensitivity 100%, specificity 97%, efficiency 98%).We conclude that: 1) high total ADA activity in tuberculous pleural effusions is due mainly to an increase in ADA-2, and, therefore, originated from the only known source monocytes and macrophages; 2) ADA-2 was a more efficient diagnostic marker of tuberculous pleurisy than total ADA activity, although the difference was not statistically significant; and 3) among effusions with high total ADA the 2'-deoxyadenosine deaminase/ADA activity ratio differentiates tuberculous effusions from empyemas and parapneumonic effusions, but fails to discriminate well between tuberculous and neoplastic effusions.

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Section: Discussionmentioning

confidence: 58%

Section: Methodsmentioning

confidence: 99%

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Adenosine deaminase (ADA) isoenzyme analysis in pleural effusions: diagnostic role, and relevance to the origin of increased ADA in tuberculous pleurisy

Valdés¹,

José²,

Álvarez³

et al. 1996

Eur Respir J

143

100

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“…IL-12 p40 was determined (in pg?mL -1 ) in 96 effusions (39 tuberculous, 42 neoplastic and 15 parapneumonic) using a sandwich ELISA from AMS Biotechnology (Abingdon, UK). ADA, expressed in U?L -1 , was determined by the method of GIUSTI [27], isoform ADA-2 was determined by inhibition with erythro-9-(2-hydroxy-3-nonyl)adenine [28], IFN-c was determined (in pg?mL ) were determined as previously described [29,30].…”

Section: Sample Collection and Analysesmentioning

confidence: 99%

Diagnostic value of interleukin-12 p40 in tuberculous pleural effusions

Valdés

José²,

Dobaño³

et al. 2009

European Respiratory Journal

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“…(5) An enzyme that participates in the metabolism of purines, ADA is also related to the proliferation of lymphocytes during the cellular response. (9)(10)(11) In addition to being a method with low operational cost, the determination of ADA levels is simple and demands little technical training, as well as using affordable reagents, glassware and equipment common to most medium-sized laboratories. (8,12) Despite the good results of the determination of ADA levels in the pleural fluid already described in the literature, (2,(13)(14)(15) it must be borne in mind that ADA levels can be elevated in cases of pleural involvement caused by rheumatoid arthritis, in some types of lymphomas and in most empyemas, as well as in cases of tuberculous effusion.…”

Section: Introductionmentioning

confidence: 99%

Avaliação da adenosina desaminase no diagnóstico da tuberculose pleural: uma metanálise brasileira

Morisson

Neves

2008

J. bras. pneumol.

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Objective: To evaluate Brazilian studies by summarizing the accuracy of adenosine deaminase in the diagnosis of the pleural tuberculosis, with the objective of lending support to the movement to make the test part of the routine in the investigation of pleural effusions. Methods: A search for Brazilian studies related to the determination of adenosine deaminase levels in the pleural liquid was carried out. These studies were evaluated and included in this study. The data were analyzed using summary receiver operating characteristic (SROC) curves, which enabled the studies to be collected and evaluated regarding the accuracy of the diagnosis. As for the global values of sensitivity and specificity, the Bayes' theorem was applied to calculate the post-test probabilities in different prevalences of the disease. Results: Twenty-five studies dating from 1987 to 2005 and including enough information to be used in the meta-analysis were identified. After evaluation, nine studies were included, totaling 1674 patients. According to the SROC curve, a sensitivity of 91.8% (95% CI: 89.8-93.6%) and a specificity of 88.4% (95% CI: 86.0-90.5%) were found, with an area of 0.969 below the curve. The overall odds ratio was 112.0 (95% CI: 51.6-243.2). Considering a prevalence of tuberculosis of 50% (considered neutral), the post-test probability was 88.7% for a positive test and 91.5% for a negative test. Conclusion: Despite the differences found among studies, it is possible to conclude that the determination of adenosine deaminase levels has high accuracy in the diagnosis of the pleural tuberculosis and should be used as a routine test in its investigation.Keywords: Pleural effusion; Tuberculosis; Diagnosis; Adenosine deaminase; Meta-analysis. ResumoObjetivo: Avaliar trabalhos brasileiros resumindo a acurácia da adenosina desaminase no diagnóstico da tuberculose pleural, com o intuito de contribuir para a concretização do exame como rotina na investigação dos derrames pleurais. Métodos: Depois de realizada uma busca por trabalhos brasileiros referentes à dosagem da adenosina desaminase no líquido pleural, estes foram avaliados e incluídos no estudo.A análise dos dados foi feita por meio da curva summary receiver operating characteristic (SROC) que possibilitou a reunião dos estudos quanto a acurácia para o diagnóstico. Com os valores globais de sensibilidade e especificidade foi aplicado o teorema de Bayes para calcular as probabilidades pós-teste em diferentes prevalências da doença. Resultados: Entre 1987 e 2005 foram encontrados 25 estudos contendo informações suficientes que poderiam ser utilizadas na metanálise. Após avaliação, foram incluídos nove estudos, totalizando 1.674 pacientes. De acordo com a curva SROC, foi encontrada uma sensibilidade de 91,8% (IC95%: 89,8-93,6%) e uma especificidade de 88,4% (IC95%: 86,0-90,5%), com uma área abaixo da curva de 0,969. O odds ratio global foi de 112,0 (IC95%: 51,6-243,2). Considerando uma prevalência da tuberculose pleural de 50% (considerada neutra), a probabilidade do di...

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Temperature Conversion Factors, Activation Energy, Relative Substrate Specificity and Optimum pH of Adenosine Deaminase from Human Serum and Tissues

Cited by 123 publications

References 12 publications

Adenosine deaminase (ADA) isoenzyme analysis in pleural effusions: diagnostic role, and relevance to the origin of increased ADA in tuberculous pleurisy

Adenosine deaminase (ADA) isoenzyme analysis in pleural effusions: diagnostic role, and relevance to the origin of increased ADA in tuberculous pleurisy

Diagnostic value of interleukin-12 p40 in tuberculous pleural effusions

Avaliação da adenosina desaminase no diagnóstico da tuberculose pleural: uma metanálise brasileira

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